Fibroblast Adaptation and Stiffness Matching to Soft Elastic Substrates
TLDR
Within a range of stiffness spanning that of soft tissues, fibroblasts tune their internal stiffness to match that of their substrate, and modulation of cellular stiffness by the rigidity of the environment may be a mechanism used to direct cell migration and wound repair.About:
This article is published in Biophysical Journal.The article was published on 2007-12-15 and is currently open access. It has received 999 citations till now. The article focuses on the topics: Stiffness.read more
Citations
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Cell mechanics of alveolar epithelial cells (AECs) and macrophages (AMs).
Sophie Féréol,Redouane Fodil,Redouane Fodil,Gabriel Pelle,Gabriel Pelle,Bruno Louis,Bruno Louis,Daniel Isabey,Daniel Isabey +8 more
TL;DR: Alveolar cells provide a pertinent model to study the biological processes governing cellular response to controlled stress or strain and the pertinence of various models used to extract cell mechanical properties is compared.
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Consideration of the Mechanical Properties of Hydrogels for Brain Tissue Engineering and Brain-on-a-chip
TL;DR: This review overviews the influence the mechanical properties of hydrogel on the behavior of brain tissue cells, and summarizes how those mechanical properties can affect cell behavior, such as viability, proliferation, differentiation, and spreading.
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Modulation of host cell mechanics by Trypanosoma cruzi
TL;DR: This work finds that cell stiffness decreases in a time‐dependent fashion in T. cruzi‐infected human foreskin fibroblasts without a significant change in the dynamics of cytoskeletal remodeling, and suggests that these changes occur through protein kinase A and inhibition of the Rho/Rho kinase signaling pathway.
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Differences in the Microrheology of Human Embryonic Stem Cells and Human Induced Pluripotent Stem Cells
Brian R. Daniels,Christopher M. Hale,Shyam B. Khatau,Sravanti Kusuma,Terrence M. Dobrowsky,Sharon Gerecht,Denis Wirtz +6 more
TL;DR: The hypothesis that intracellular elasticity correlates with the degree of cellular differentiation and reveals significant differences in the mechanical properties of hiPS cells and hES cells is confirmed and the concept that stem cell "softness" is a key feature of force-mediated differentiation of stem cells is supported.
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The homeostatic ensemble for cells
TL;DR: This work develops a statistical mechanics framework for living cells by including the homeostatic constraint that exists over the interphase period of the cell cycle, and argues that the observed variabilities are inherent to the entropic nature of the homestatic equilibrium of cells and not a result of in vitro experimental errors.
References
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Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
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Tensional homeostasis and the malignant phenotype.
Matthew J. Paszek,Nastaran Zahir,Kandice R. Johnson,Johnathon N. Lakins,Gabriela I. Rozenberg,Amit Gefen,Cynthia A. Reinhart-King,Susan S. Margulies,Micah Dembo,David Boettiger,Daniel A. Hammer,Valerie M. Weaver +11 more
TL;DR: It is found that tumors are rigid because they have a stiff stroma and elevated Rho-dependent cytoskeletal tension that drives focal adhesions, disrupts adherens junctions, perturbs tissue polarity, enhances growth, and hinders lumen formation.
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Cell Movement Is Guided by the Rigidity of the Substrate
TL;DR: It is discovered that changes in tissue rigidity and strain could play an important controlling role in a number of normal and pathological processes involving cell locomotion, including morphogenesis, the immune response, and wound healing.
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Cell locomotion and focal adhesions are regulated by substrate flexibility
Robert J. Pelham,Yu-li Wang +1 more
TL;DR: The ability of cells to survey the mechanical properties of their surrounding environment is demonstrated and the possible involvement of both protein tyrosine phosphorylation and myosin-generated cortical forces in this process is suggested.