Fibroblast Adaptation and Stiffness Matching to Soft Elastic Substrates
TLDR
Within a range of stiffness spanning that of soft tissues, fibroblasts tune their internal stiffness to match that of their substrate, and modulation of cellular stiffness by the rigidity of the environment may be a mechanism used to direct cell migration and wound repair.About:
This article is published in Biophysical Journal.The article was published on 2007-12-15 and is currently open access. It has received 999 citations till now. The article focuses on the topics: Stiffness.read more
Citations
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Investigation of quercetin-induced HepG2 cell apoptosis-associated cellular biophysical alterations by atomic force microscopy
Jiang Pi,Baole Li,Lv-Ying Tu,Haiyan Zhu,Hua Jin,Fen Yang,Haihua Bai,Huaihong Cai,Jiye Cai,Jiye Cai +9 more
TL;DR: It is suggested that quercetin can be served as a potential therapeutic agent for HCC, which not only extends the understanding of the anticancer effects of quercETin against HCC cells into nanoscale, but also highlights the applications of AFM for the investigation of anticancer drugs.
Journal ArticleDOI
Compliant substratum modulates vinculin expression in focal adhesion plaques in skeletal cells.
TL;DR: The potential correlation between microenvironmental mechanics and the skeletal system is established, and the results regarding changes in cell spreading area, cytoskeleton and cell adhesion further indicate the important role of biomechanics in the cell-matrix interaction.
Posted ContentDOI
Nuclear decoupling is part of a rapid protein-level cellular response to high-intensity mechanical loading
Hamish T. J. Gilbert,Venkatesh Mallikarjun,Oana Dobre,Mark R. Jackson,Robert Pedley,Andrew P. Gilmore,Stephen M. Richardson,Joe Swift +7 more
TL;DR: The time-dependent response of primary human mesenchymal stem cells to cyclic tensile strain was examined to decouple mechano-transmission between the cytoskeleton and the nucleus, thus conferring protection to chromatin.
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Three-dimensional in vitro culture models in oncology research
Camille Jubelin,J. Muñoz-García,Laurent Griscom,Denis Cochonneau,Emilie Ollivier,Marie-Françoise Heymann,François M. Vallette,Lisa Oliver,Dominique Heymann +8 more
TL;DR: A review of 3D cell culture models for cancer development and drug response can be found in this article , where the advantages and limitations of these models and their techniques of production are discussed.
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Matrix promote mesenchymal stromal cell migration with improved deformation via nuclear stiffness decrease.
Chuanchuan Lin,Bailong Tao,Yiman Deng,Ye He,Xinkun Shen,Rong Wang,Lu Lu,Zhihong Peng,Zengzilu Xia,Kaiyong Cai +9 more
TL;DR: In this work, sodium alginate hydrogels with different stiffness and Transwell chambers with gradient chemical factors were employed to mimic tissue repair in vivo and it was found that BMSCs fitted environment by selecting migration strategy.
References
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Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
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Tensional homeostasis and the malignant phenotype.
Matthew J. Paszek,Nastaran Zahir,Kandice R. Johnson,Johnathon N. Lakins,Gabriela I. Rozenberg,Amit Gefen,Cynthia A. Reinhart-King,Susan S. Margulies,Micah Dembo,David Boettiger,Daniel A. Hammer,Valerie M. Weaver +11 more
TL;DR: It is found that tumors are rigid because they have a stiff stroma and elevated Rho-dependent cytoskeletal tension that drives focal adhesions, disrupts adherens junctions, perturbs tissue polarity, enhances growth, and hinders lumen formation.
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Cell Movement Is Guided by the Rigidity of the Substrate
TL;DR: It is discovered that changes in tissue rigidity and strain could play an important controlling role in a number of normal and pathological processes involving cell locomotion, including morphogenesis, the immune response, and wound healing.
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Cell locomotion and focal adhesions are regulated by substrate flexibility
Robert J. Pelham,Yu-li Wang +1 more
TL;DR: The ability of cells to survey the mechanical properties of their surrounding environment is demonstrated and the possible involvement of both protein tyrosine phosphorylation and myosin-generated cortical forces in this process is suggested.