Fibroblast Adaptation and Stiffness Matching to Soft Elastic Substrates
TLDR
Within a range of stiffness spanning that of soft tissues, fibroblasts tune their internal stiffness to match that of their substrate, and modulation of cellular stiffness by the rigidity of the environment may be a mechanism used to direct cell migration and wound repair.About:
This article is published in Biophysical Journal.The article was published on 2007-12-15 and is currently open access. It has received 999 citations till now. The article focuses on the topics: Stiffness.read more
Citations
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Force generation upon T cell receptor engagement.
Julien Husson,Karine Chemin,Karine Chemin,Armelle Bohineust,Armelle Bohineust,Claire Hivroz,Claire Hivroz,Nelly Henry,Nelly Henry,Nelly Henry +9 more
TL;DR: The first quantified description of force generation sequence upon local bidimensional engagement of TCR-CD3 is provided and its potential role in a T cell mechanically-regulated activation process is discussed.
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Patterning the differentiation of C2C12 skeletal myoblasts.
Piyush Bajaj,Bobby Reddy,Larry J. Millet,Chunan Wei,Pinar Zorlutuna,Pinar Zorlutuna,Gang Bao,Rashid Bashir +7 more
TL;DR: It is shown that the geometrical cues of substrates can significantly influence the differentiation process of C2C12 skeletal myoblasts and can have important implications in engineering skeletal muscle tissues and designing muscle cell bio-actuators.
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Physically based principles of cell adhesion mechanosensitivity in tissues
TL;DR: How biophysical approaches, both experimentally and theoretically, have contributed to the understanding of the regulation of cellular functions through physical force sensing mechanisms are shown.
Laminin and biomimetic extracellular elasticity enhance functional differentiation in mammary epithelia - eScholarship
TL;DR: In the mammary gland, epithelial cells are embedded in a soft environment and become functionally differentiated in culture when exposed to a laminin-rich extracellular matrix gel as discussed by the authors.
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Cells Actively Stiffen Fibrin Networks by Generating Contractile Stress
TL;DR: It is proposed that stiffening is a consequence of active myosin-driven cell contraction, which provokes a nonlinear elastic response of the fibrin matrix, which may provide design parameters for materials to guide morphogenesis in tissue engineering.
References
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Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
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Tensional homeostasis and the malignant phenotype.
Matthew J. Paszek,Nastaran Zahir,Kandice R. Johnson,Johnathon N. Lakins,Gabriela I. Rozenberg,Amit Gefen,Cynthia A. Reinhart-King,Susan S. Margulies,Micah Dembo,David Boettiger,Daniel A. Hammer,Valerie M. Weaver +11 more
TL;DR: It is found that tumors are rigid because they have a stiff stroma and elevated Rho-dependent cytoskeletal tension that drives focal adhesions, disrupts adherens junctions, perturbs tissue polarity, enhances growth, and hinders lumen formation.
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Cell Movement Is Guided by the Rigidity of the Substrate
TL;DR: It is discovered that changes in tissue rigidity and strain could play an important controlling role in a number of normal and pathological processes involving cell locomotion, including morphogenesis, the immune response, and wound healing.
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Cell locomotion and focal adhesions are regulated by substrate flexibility
Robert J. Pelham,Yu-li Wang +1 more
TL;DR: The ability of cells to survey the mechanical properties of their surrounding environment is demonstrated and the possible involvement of both protein tyrosine phosphorylation and myosin-generated cortical forces in this process is suggested.