Fibroblast Adaptation and Stiffness Matching to Soft Elastic Substrates
TLDR
Within a range of stiffness spanning that of soft tissues, fibroblasts tune their internal stiffness to match that of their substrate, and modulation of cellular stiffness by the rigidity of the environment may be a mechanism used to direct cell migration and wound repair.About:
This article is published in Biophysical Journal.The article was published on 2007-12-15 and is currently open access. It has received 999 citations till now. The article focuses on the topics: Stiffness.read more
Citations
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Swelling and mechanical properties of alginate hydrogels with respect to promotion of neural growth.
TL;DR: Soft Ca-alginate NaCl hydrogels combine mechanical stability in solutions of high ionic strength with the ability to support neural growth and could be useful as 3D implants for neural regeneration in vivo.
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Aging and anatomical variations in lung tissue stiffness.
Delphine Sicard,Andrew J. Haak,Kyoung Moo Choi,Alexandria R. Craig,Laura E. Fredenburgh,Daniel J. Tschumperlin +5 more
TL;DR: Age-related changes in tissue mechanical properties that likely contribute to impaired lung function with aging are demonstrated and the potential to identify mechanisms that contribute to mechanical tissue remodeling through the study of human cells and tissues from across the aging spectrum is underscored.
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Computational modeling of three-dimensional ECM-rigidity sensing to guide directed cell migration
Mincheol Kim,Yaron R. Silberberg,Rohan Abeyaratne,Roger D. Kamm,Roger D. Kamm,H. Harry Asada,H. Harry Asada +6 more
TL;DR: A method for characterizing the stiffness of ECM that is sensed by filopodia based on the theory of elasticity and discrete ECM fiber is presented and applied to a Filopodial mechanosensing model for predicting directed cell migration toward stiffer ECM.
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Extracellular matrix rigidity modulates neuroblastoma cell differentiation and N-myc expression
Wilbur A. Lam,Wilbur A. Lam,Lizhi Cao,Vaibhavi Umesh,Albert J. Keung,Shamik Sen,Sanjay Kumar +6 more
TL;DR: It is found that increasing ECM stiffness enhances neuritogenesis and suppresses cell proliferation and reduces expression of N-Myc, a transcription factor involved in multiple aspects of oncogenic proliferation that is used for evaluating prognosis and clinical grading of neuroblastoma.
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Cell–Extracellular Matrix Mechanobiology: Forceful Tools and Emerging Needs for Basic and Translational Research
Andrew W. Holle,Andrew W. Holle,Jennifer L. Young,Jennifer L. Young,Krystyn J. Van Vliet,Roger D. Kamm,Dennis E. Discher,Paul A. Janmey,Joachim P. Spatz,Joachim P. Spatz,M. Taher A. Saif +10 more
TL;DR: Interactions between cell and matrix mechanics in vivo are of particular importance in a wide variety of disorders, including cancer, central nervous system injury, fibrotic diseases, and myocardial infarction.
References
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Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
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Tensional homeostasis and the malignant phenotype.
Matthew J. Paszek,Nastaran Zahir,Kandice R. Johnson,Johnathon N. Lakins,Gabriela I. Rozenberg,Amit Gefen,Cynthia A. Reinhart-King,Susan S. Margulies,Micah Dembo,David Boettiger,Daniel A. Hammer,Valerie M. Weaver +11 more
TL;DR: It is found that tumors are rigid because they have a stiff stroma and elevated Rho-dependent cytoskeletal tension that drives focal adhesions, disrupts adherens junctions, perturbs tissue polarity, enhances growth, and hinders lumen formation.
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Cell Movement Is Guided by the Rigidity of the Substrate
TL;DR: It is discovered that changes in tissue rigidity and strain could play an important controlling role in a number of normal and pathological processes involving cell locomotion, including morphogenesis, the immune response, and wound healing.
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Cell locomotion and focal adhesions are regulated by substrate flexibility
Robert J. Pelham,Yu-li Wang +1 more
TL;DR: The ability of cells to survey the mechanical properties of their surrounding environment is demonstrated and the possible involvement of both protein tyrosine phosphorylation and myosin-generated cortical forces in this process is suggested.