Fibroblast Adaptation and Stiffness Matching to Soft Elastic Substrates
TLDR
Within a range of stiffness spanning that of soft tissues, fibroblasts tune their internal stiffness to match that of their substrate, and modulation of cellular stiffness by the rigidity of the environment may be a mechanism used to direct cell migration and wound repair.About:
This article is published in Biophysical Journal.The article was published on 2007-12-15 and is currently open access. It has received 999 citations till now. The article focuses on the topics: Stiffness.read more
Citations
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Dissertation
Biomechanical modelling of colorectal crypt formation and in-vitro replication
TL;DR: Modelling growth parametrically as a sequence of equilibrium configurations attained by beams of increasing length shows that competition between lateral supports and stromal adhesion determines buckling wavelength, and shows how non-equilibrium relaxation of tethering forces affects post-buckled shapes, and illustrates that growth inhomogeneity has a much weaker influence upon buckled configurations than do variations of mechanical properties.
Journal ArticleDOI
A Stiff Extracellular Matrix Favors the Mechanical Cell Competition that Leads to Extrusion of Bacterially-Infected Epithelial Cells
TL;DR: The results suggest that ECM stiffness plays a major role in modulating the competition between infected and uninfected cells, with stiffer matrices promoting this battle through differential modulation of cell mechanics between the two cell populations.
Journal ArticleDOI
A micro-mechanical device for in-situ stretching of single cells cultured on it
Somanna A. Kollimada,Sreenath Balakrishnan,Charanjeet Kaur Malhi,Shilpa R. Raju,M. S. Suma,Saumitra Das,G. K. Ananthasuresh +6 more
TL;DR: The utility of the micro-mechanical device for providing a mechanical stimulus to cells cultured on it and observing the change in the deformation of the nucleus of the cell is demonstrated by culturing NIH 3T3 fibroblasts on the devices, stretching them in situ, and measuring theDeformation of their nuclei using fluorescence imaging.
The mechanotransduction of hydrostatic pressure by mesenchymal stem cells
TL;DR: This study demonstrates that a complex relationship between cell-matrix and HP is demonstrated, and MSCs seeded into either agarose or fibrin hydrogels and exposed to cyclic HP were found to support a spherical cellular morphology.
Book ChapterDOI
Biophysical origins of viscoelasticity during collective cell migration
TL;DR: This chapter will focus on how molecular interactions of the actin cytoskeleton, cellular adhesions, and biological substrates give rise to larger scale viscoelastic behavior and how tissue vis coelasticity affects collective migration dynamics.
References
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Journal ArticleDOI
Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
Tensional homeostasis and the malignant phenotype.
Matthew J. Paszek,Nastaran Zahir,Kandice R. Johnson,Johnathon N. Lakins,Gabriela I. Rozenberg,Amit Gefen,Cynthia A. Reinhart-King,Susan S. Margulies,Micah Dembo,David Boettiger,Daniel A. Hammer,Valerie M. Weaver +11 more
TL;DR: It is found that tumors are rigid because they have a stiff stroma and elevated Rho-dependent cytoskeletal tension that drives focal adhesions, disrupts adherens junctions, perturbs tissue polarity, enhances growth, and hinders lumen formation.
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Cell Movement Is Guided by the Rigidity of the Substrate
TL;DR: It is discovered that changes in tissue rigidity and strain could play an important controlling role in a number of normal and pathological processes involving cell locomotion, including morphogenesis, the immune response, and wound healing.
Journal ArticleDOI
Cell locomotion and focal adhesions are regulated by substrate flexibility
Robert J. Pelham,Yu-li Wang +1 more
TL;DR: The ability of cells to survey the mechanical properties of their surrounding environment is demonstrated and the possible involvement of both protein tyrosine phosphorylation and myosin-generated cortical forces in this process is suggested.