Fibroblast Adaptation and Stiffness Matching to Soft Elastic Substrates
TLDR
Within a range of stiffness spanning that of soft tissues, fibroblasts tune their internal stiffness to match that of their substrate, and modulation of cellular stiffness by the rigidity of the environment may be a mechanism used to direct cell migration and wound repair.About:
This article is published in Biophysical Journal.The article was published on 2007-12-15 and is currently open access. It has received 999 citations till now. The article focuses on the topics: Stiffness.read more
Citations
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Journal ArticleDOI
Cell adhesion mechanisms on laterally mobile polymer films.
TL;DR: Insight is yielded into ECM mechanosensing by revealing that cells can engage distinct mechanisms to promote adhesion onto substrates with different time-dependent properties.
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An XFEM‐based numerical strategy to model mechanical interactions between biological cells and a deformable substrate
Mehdi Farsad,Franck J. Vernerey +1 more
TL;DR: A computational framework, based on the extended FEM (XFEM) and the level set method, to model the evolution of two‐dimensional cells lying on an elastic substrate whose properties can be varied and which provides a flexible platform that can handle complex cell geometries.
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Schwann cell durotaxis can be guided by physiologically relevant stiffness gradients.
TL;DR: It is revealed that Schwann cells can follow extracellular gradients of increasing stiffness, in a form of directed migration termed durotaxis, and further shows that the presence of a steep stiffness gradient can support a pro-regenerative cell morphology.
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Cell migration and organization in three-dimensional in vitro culture driven by stiffness gradient.
Danielle N. Joaquin,Michael Grigola,Gubeum Kwon,Christopher Blasius,Yutao Han,Daniel Perlitz,Jing Jiang,Yvonne S. Ziegler,Ann M. Nardulli,K. Jimmy Hsia +9 more
TL;DR: The durotaxis results show that the cell migration velocity does not have any consistency with the stiffness of the substrate, rather it is more related to the stiffness gradient of the slab, suggesting a new mechanism underlying the duroTaxis phenomenon.
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Development and characterization of cholangioids from normal and diseased human cholangiocytes as an in vitro model to study primary sclerosing cholangitis
Lorena Loarca,Thiago M. De Assuncao,Nidhi Jalan-Sakrikar,Steve F. Bronk,Anuradha Krishnan,Bing Huang,Leslie Morton,Christy E. Trussoni,Lorena Marcano Bonilla,Eugene W. Krueger,Steve P. O'Hara,Patrick L. Splinter,Guang Shi,Maria J. Lorenzo Pisarello,Gregory J. Gores,Robert C. Huebert,Nicholas F. LaRusso +16 more
TL;DR: A method to generate normal and diseased cholangioids is developed, characterized them morphologically and functionally, and showed that they can be induced to senescence and SASP, and demonstrated both EV release and macrophage attraction.
References
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Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
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Tensional homeostasis and the malignant phenotype.
Matthew J. Paszek,Nastaran Zahir,Kandice R. Johnson,Johnathon N. Lakins,Gabriela I. Rozenberg,Amit Gefen,Cynthia A. Reinhart-King,Susan S. Margulies,Micah Dembo,David Boettiger,Daniel A. Hammer,Valerie M. Weaver +11 more
TL;DR: It is found that tumors are rigid because they have a stiff stroma and elevated Rho-dependent cytoskeletal tension that drives focal adhesions, disrupts adherens junctions, perturbs tissue polarity, enhances growth, and hinders lumen formation.
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Cell Movement Is Guided by the Rigidity of the Substrate
TL;DR: It is discovered that changes in tissue rigidity and strain could play an important controlling role in a number of normal and pathological processes involving cell locomotion, including morphogenesis, the immune response, and wound healing.
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Cell locomotion and focal adhesions are regulated by substrate flexibility
Robert J. Pelham,Yu-li Wang +1 more
TL;DR: The ability of cells to survey the mechanical properties of their surrounding environment is demonstrated and the possible involvement of both protein tyrosine phosphorylation and myosin-generated cortical forces in this process is suggested.