Fibroblast Adaptation and Stiffness Matching to Soft Elastic Substrates
TLDR
Within a range of stiffness spanning that of soft tissues, fibroblasts tune their internal stiffness to match that of their substrate, and modulation of cellular stiffness by the rigidity of the environment may be a mechanism used to direct cell migration and wound repair.About:
This article is published in Biophysical Journal.The article was published on 2007-12-15 and is currently open access. It has received 999 citations till now. The article focuses on the topics: Stiffness.read more
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The influence and interactions of substrate thickness, organization and dimensionality on cell morphology and migration.
TL;DR: This work generates cell-laden three-dimensional collagen hydrogels with aligned collagen fibrils using a simple microfluidic device driven by hydrostatic flow and shows the importance of well-controlled physical parameters in the cellular microenvironment.
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Biomechanical imaging of cell stiffness and prestress with subcellular resolution
Elizabeth P. Canović,D. Thomas Seidl,Samuel R. Polio,Assad A. Oberai,Paul E. Barbone,Dimitrije Stamenović,Michael L. Smith +6 more
TL;DR: Bomechanical imaging offers a new tool that can be used in studies of cell biomechanics and mechanobiology where fast imaging of cell properties and prestress is desired at subcellular spatial resolution.
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Substrate Fluidity Regulates Cell Adhesion and Morphology on Poly(ε-caprolactone)-Based Materials
TL;DR: It was found that the substrate stiffness produced little changes in cell spread area, indicating that cells sense more dynamic nature of their surrounding environment.
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Spontaneous buckling of contractile poroelastic actomyosin sheets.
Yaron Ideses,Vitaly Erukhimovitch,R. Brand,D. Jourdain,J. Salmeron Hernandez,Uri R. Gabinet,Samuel A. Safran,Karsten Kruse,Karsten Kruse,Anne Bernheim-Groswasser +9 more
TL;DR: In this paper, the authors study the contraction and buckling of active, initially homogeneous, thin elastic actomyosin networks isolated from bounding surfaces, where a flow of fluid is generated during contraction.
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Nanoscale Mechanosensing of Natural Killer Cells is Revealed by Antigen-Functionalized Nanowires.
TL;DR: A mechanosensing mechanism of NK cells is proposed, by which they integrate biochemical and mechanical stimuli into a decision‐making machinery analogous to the AND logic gate, whose output is the immune activation.
References
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Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
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Tensional homeostasis and the malignant phenotype.
Matthew J. Paszek,Nastaran Zahir,Kandice R. Johnson,Johnathon N. Lakins,Gabriela I. Rozenberg,Amit Gefen,Cynthia A. Reinhart-King,Susan S. Margulies,Micah Dembo,David Boettiger,Daniel A. Hammer,Valerie M. Weaver +11 more
TL;DR: It is found that tumors are rigid because they have a stiff stroma and elevated Rho-dependent cytoskeletal tension that drives focal adhesions, disrupts adherens junctions, perturbs tissue polarity, enhances growth, and hinders lumen formation.
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Cell Movement Is Guided by the Rigidity of the Substrate
TL;DR: It is discovered that changes in tissue rigidity and strain could play an important controlling role in a number of normal and pathological processes involving cell locomotion, including morphogenesis, the immune response, and wound healing.
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Cell locomotion and focal adhesions are regulated by substrate flexibility
Robert J. Pelham,Yu-li Wang +1 more
TL;DR: The ability of cells to survey the mechanical properties of their surrounding environment is demonstrated and the possible involvement of both protein tyrosine phosphorylation and myosin-generated cortical forces in this process is suggested.