Journal ArticleDOI
Generation and analysis of interleukin-4 deficient mice.
TLDR
Some but not all of the in vitro properties of IL-4 are critical for the physiology of the immune system in vivo, but the serum levels of IgG1 and IgE are strongly reduced.Abstract:
Interleukin-4 (IL-4) promotes the growth and differentiation of many hematopoietic cells in vitro; in particular, it directs the immunoglobulin (Ig) class switch to IgG1 and IgE. Mice homozygous for a mutation that inactivates the IL-4 gene were generated to test the requirement for IL-4 in vivo. In the mutant mice T and B cell development was normal, but the serum levels of IgG1 and IgE were strongly reduced. The IgG1 dominance in a T cell-dependent immune response was lost, and IgE was not detectable upon nematode infection. Thus, some but not all of the in vitro properties of IL-4 are critical for the physiology of the immune system in vivo.read more
Citations
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Journal ArticleDOI
Functional diversity of helper T lymphocytes.
TL;DR: The existence of subsets of CD4+ helper T lymphocytes that differ in their cytokine secretion patterns and effector functions provides a framework for understanding the heterogeneity of normal and pathological immune responses.
Journal ArticleDOI
Interleukin-10-deficient mice develop chronic enterocolitis
TL;DR: The results indicate that the bowel inflammation in the mutants originates from uncontrolled immune responses stimulated by enteric antigens and that IL-10 is an essential immunoregulator in the intestinal tract.
Journal ArticleDOI
Insulin-promoter-factor 1 is required for pancreas development in mice
TL;DR: The findings show that IPF1 is needed for the formation of the pancreas and suggest that it acts to determine the fate of common pancreatic precursor cells and/ or to regulate their propagation.
Journal ArticleDOI
Stat6 Is Required for Mediating Responses to IL-4 and for the Development of Th2 Cells
TL;DR: It is demonstrated that, despite the existence of multiple signaling pathways activated by IL-4, Stat6 is essential for mediating responses toIL-4 lymphocytes.
References
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Journal ArticleDOI
T cell-derived B cell differentiation factor(s). Effect on the isotype switch of murine B cells.
TL;DR: The results suggest that soluble factors produced by T cell lines and hybridomas can markedly influence both the class and subclass of Ig produced by B cells.
Journal ArticleDOI
Suppression of in vivo polyclonal IgE responses by monoclonal antibody to the lymphokine B-cell stimulatory factor 1.
TL;DR: Results indicate an important physiologic role for BSF-1 in the generation of IgE antibody responses and suggest means for limiting the production of antibodies responsible for allergic reactions without inhibiting protective antibody responses.
Journal ArticleDOI
B cell stimulatory factor-1/interleukin-4 mRNA is expressed by normal and transformed mast cells
Melissa A. Brown,Jacalyn H. Pierce,Cynthia J. Watson,Joseph Falco,James N. Ihle,William E. Paul +5 more
TL;DR: It is demonstrated here that BSF-1 mRNA is expressed by a majority of transformed mast cell lines and by 5 IL-3-dependent non-transformed mast cell Lines, indicating that mast cells may be an important physiologic source of this factor.
Journal ArticleDOI
Interleukin 4 (B-cell stimulatory factor 1) can enhance or antagonize the factor-dependent growth of hemopoietic progenitor cells.
Donna Rennick,Gloria Yang,Christa Muller-Sieburg,Craig P. Smith,Naoko Arai,Yutaka Takabe,Lorraine Gemmell +6 more
TL;DR: It is found that IL-4 inhibits stromal cell-dependent growth of bone marrow-derived pre-B cells and appears to inhibit the in vitro generation of colony-forming progenitor cells from immature IL-3-dependent stem cells.
Journal ArticleDOI
A mutant neomycin phosphotransferase II gene reduces the resistance of transformants to antibiotic selection pressure.
TL;DR: The neo (neomycin-resistance) gene of transposon Tn5 encodes the enzyme neomycin phosphotransferase II, which confers resistance to various aminoglycoside antibiotics, including kanamycin and G418.
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