Journal ArticleDOI
Generation and analysis of interleukin-4 deficient mice.
TLDR
Some but not all of the in vitro properties of IL-4 are critical for the physiology of the immune system in vivo, but the serum levels of IgG1 and IgE are strongly reduced.Abstract:
Interleukin-4 (IL-4) promotes the growth and differentiation of many hematopoietic cells in vitro; in particular, it directs the immunoglobulin (Ig) class switch to IgG1 and IgE. Mice homozygous for a mutation that inactivates the IL-4 gene were generated to test the requirement for IL-4 in vivo. In the mutant mice T and B cell development was normal, but the serum levels of IgG1 and IgE were strongly reduced. The IgG1 dominance in a T cell-dependent immune response was lost, and IgE was not detectable upon nematode infection. Thus, some but not all of the in vitro properties of IL-4 are critical for the physiology of the immune system in vivo.read more
Citations
More filters
Journal ArticleDOI
Tissue-specific and mosaic imprinting defects underlie opposite congenital growth disorders in mice.
Andrea Freschi,Stella K. Hur,Federica Maria Valente,Folami Y. Ideraabdullah,Angela Sparago,Maria Teresa Gentile,Andrea Oneglia,Diego Di Nucci,Luca Colucci-D'Amato,Joanne L. Thorvaldsen,Marisa S. Bartolomei,Andrea Riccio,Flavia Cerrato +12 more
TL;DR: Tissue-specific imprinting defects result in deficient liver- and placenta-derived Igf2 on paternal transmission and excessive IgF2 in peripheral tissues on maternal transmission, providing a possible molecular explanation for imprinting-associated and phenotypically contrasting growth disorders.
Journal ArticleDOI
Features of B Cell Responses Relevant to Allergic Disease
TL;DR: In this article , the basic principles of B cell differentiation and features relevant to allergic immune responses are discussed, including class-switch recombination, plasma cell differentiation, germinal centers and affinity maturation, memory B cells and recall responses, with an emphasis on IgE, IgG1 and IgG4.
Journal ArticleDOI
Interleukin 4 targeting of immunoglobulin heavy chain class-switch recombination.
Journal ArticleDOI
CD1d-Independent Developmental Acquisition of Prompt IL-4 Gene Inducibility in Thymus CD161(NK1)−CD44lowCD4+CD8− T Cells Is Associated with Complementarity Determining Region 3-Diverse and Biased Vβ2/Vβ7/Vβ8/Vα3.2 T Cell Receptor Usage
Yi-Ting Chen,John T. Kung +1 more
TL;DR: It is shown that thymus CD161−CD44lowCD4+CD8− T cells promptly produce IL-4 upon TCR stimulation, a response that displays biased Vβ(2/7/8) and Vα3.2 TCR usage, demonstrating the existence of a NKT cell distinct, TCR-repertoire diverse naive CD4+ T cell subset capable of prompt IL- 4 inducibility.
Journal ArticleDOI
Differentiation of human single-positive fetal thymocytes in vitro into IL-4- and/or IFN-gamma-producing CD4+ and CD8+ T cells.
TL;DR: The full capacity of fetal human thymocytes to differentiate into cytokine-producing T cells in a priming milieu with appropriate stimulatory molecules and exogenous cytokines is demonstrated.
References
More filters
Journal ArticleDOI
TH1 and TH2 cells: different patterns of lymphokine secretion lead to different functional properties.
Tim R. Mosmann,R L Coffman +1 more
TL;DR: Two types of cloned helper T cells are described, defined primarily by differences in the pattern of lymphokines ynthesized, and the different functions of the two types of cells and their lymphokine synthesis are discussed.
Journal ArticleDOI
Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells.
Kirk R. Thomas,Mario R. Capecchi +1 more
TL;DR: This work mutated, by gene targeting, the endogenous hypoxanthine phosphoribosyl transferase (HPRT) gene in mouse embryo-derived stem (ES) cells and compared the gene-targeting efficiencies of two classes of neor-Hprt recombinant vectors.
Journal ArticleDOI
Altering the genome by homologous recombination.
TL;DR: The current status of gene targeting with particular emphasis on germ line modification of the mouse genome is discussed, and the different methods so far employed to identify those rare embryonic stem cells in which the desired targeting event has occurred are described.
Journal ArticleDOI
Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells
Rachel Williams,D J Hilton,S Pease,Tracy A. Willson,Colin L. Stewart,David P. Gearing,Erwin F. Wagner,D. Metcalf,Nicos A. Nicola,Nicholas M. Gough +9 more
TL;DR: In this paper, a recombinant myeloid leukaemia inhibitory factor (LIF) was used to replace DIA in the maintenance of totipotent ES cell lines that retain the potential to form chimaeric mice.
Journal ArticleDOI
Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens.
TL;DR: This paper used hybridoma monoclonal antibodies obtained after immunization of mice with rat cells to study rat cell-surface antigens present on subpopulations of rat lymphocytes.
Related Papers (5)
TH1 and TH2 cells: different patterns of lymphokine secretion lead to different functional properties.
Tim R. Mosmann,R L Coffman +1 more