Journal ArticleDOI
Generation and analysis of interleukin-4 deficient mice.
TLDR
Some but not all of the in vitro properties of IL-4 are critical for the physiology of the immune system in vivo, but the serum levels of IgG1 and IgE are strongly reduced.Abstract:
Interleukin-4 (IL-4) promotes the growth and differentiation of many hematopoietic cells in vitro; in particular, it directs the immunoglobulin (Ig) class switch to IgG1 and IgE. Mice homozygous for a mutation that inactivates the IL-4 gene were generated to test the requirement for IL-4 in vivo. In the mutant mice T and B cell development was normal, but the serum levels of IgG1 and IgE were strongly reduced. The IgG1 dominance in a T cell-dependent immune response was lost, and IgE was not detectable upon nematode infection. Thus, some but not all of the in vitro properties of IL-4 are critical for the physiology of the immune system in vivo.read more
Citations
More filters
Journal ArticleDOI
The effect of safranal, a constituent of Crocus sativus (saffron), on tracheal responsiveness, serum levels of cytokines, total NO and nitrite in sensitized guinea pigs
TL;DR: These results showed a preventive effect for safranal on tracheal responses and serum cytokine, total NO and nitrite levels as well as increased Th1/Th2 balance in sensitized guinea pigs.
Journal ArticleDOI
Humanized H19/Igf2 locus reveals diverged imprinting mechanism between mouse and human and reflects Silver–Russell syndrome phenotypes
Stella K. Hur,Andrea Freschi,Folami Y. Ideraabdullah,Joanne L. Thorvaldsen,Lacey J. Luense,Angela H. Weller,Shelley L. Berger,Flavia Cerrato,Andrea Riccio,Marisa S. Bartolomei +9 more
TL;DR: The conserved maternal imprinting mechanism and function at IC1 demonstrates the possibility of modeling maternal transmission of hIC1 mutations associated with BWS in mice, and it is proposed that further analyses in the paternal knock-in H19+/hIC1 mice will elucidate the molecular mechanisms that may underlie SRS.
Journal ArticleDOI
Murine metal-induced systemic autoimmunity : baseline and stimulated cytokine mRNA expression in genetically susceptible and resistant strains
Bo Häggqvist,Per Hultman +1 more
TL;DR: H‐2‐linked resistance to Hg‐induced AFA is characterized by low constitutive expression of IL‐2 and IFN‐γ mRNA, which is not increased by Hg, and a marked increase in IL‐10 expression.
Journal ArticleDOI
Bmp Indicator Mice Reveal Dynamic Regulation of Transcriptional Response
Anna L. Javier,Linda Doan,Mui Luong,N. Soledad Reyes de Mochel,Aixu Sun,Edwin S. Monuki,Ken W.Y. Cho +6 more
TL;DR: A reporter identifies specific transcriptional responses that are mediated by Smad1 and Smad4 with the Schnurri transcription factor complex binding to a conserved Bmp-Responsive Element (BRE), originally identified among Drosophila, Xenopus and human Bmp targets.
Journal ArticleDOI
Control of allergic reactions in mice by an active anti-murine IL-4 immunization
Hélène Le Buanec,Sébastien Paturance,Isabelle Couillin,Silvia Schnyder-Candrian,Patrick Larcier,Bernard Ryffel,Bernard Bizzini,Armand Bensussan,Arsène Burny,Robert C. Gallo,Daniel Zagury,Gabriel Peltre +11 more
TL;DR: MIL-4 kinoid immunization reversed the allergic IgE:IgG ratio hallmark in rBet v 1a sensitized mice and reduced pulmonary eosinophil recruitment and bronchial hyperreactivity in Ova-sensitized mice, paving the way to alternative therapies to combat allergic conditions.
References
More filters
Journal ArticleDOI
TH1 and TH2 cells: different patterns of lymphokine secretion lead to different functional properties.
Tim R. Mosmann,R L Coffman +1 more
TL;DR: Two types of cloned helper T cells are described, defined primarily by differences in the pattern of lymphokines ynthesized, and the different functions of the two types of cells and their lymphokine synthesis are discussed.
Journal ArticleDOI
Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells.
Kirk R. Thomas,Mario R. Capecchi +1 more
TL;DR: This work mutated, by gene targeting, the endogenous hypoxanthine phosphoribosyl transferase (HPRT) gene in mouse embryo-derived stem (ES) cells and compared the gene-targeting efficiencies of two classes of neor-Hprt recombinant vectors.
Journal ArticleDOI
Altering the genome by homologous recombination.
TL;DR: The current status of gene targeting with particular emphasis on germ line modification of the mouse genome is discussed, and the different methods so far employed to identify those rare embryonic stem cells in which the desired targeting event has occurred are described.
Journal ArticleDOI
Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells
Rachel Williams,D J Hilton,S Pease,Tracy A. Willson,Colin L. Stewart,David P. Gearing,Erwin F. Wagner,D. Metcalf,Nicos A. Nicola,Nicholas M. Gough +9 more
TL;DR: In this paper, a recombinant myeloid leukaemia inhibitory factor (LIF) was used to replace DIA in the maintenance of totipotent ES cell lines that retain the potential to form chimaeric mice.
Journal ArticleDOI
Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens.
TL;DR: This paper used hybridoma monoclonal antibodies obtained after immunization of mice with rat cells to study rat cell-surface antigens present on subpopulations of rat lymphocytes.
Related Papers (5)
TH1 and TH2 cells: different patterns of lymphokine secretion lead to different functional properties.
Tim R. Mosmann,R L Coffman +1 more