Journal ArticleDOI
Generation and analysis of interleukin-4 deficient mice.
TLDR
Some but not all of the in vitro properties of IL-4 are critical for the physiology of the immune system in vivo, but the serum levels of IgG1 and IgE are strongly reduced.Abstract:
Interleukin-4 (IL-4) promotes the growth and differentiation of many hematopoietic cells in vitro; in particular, it directs the immunoglobulin (Ig) class switch to IgG1 and IgE. Mice homozygous for a mutation that inactivates the IL-4 gene were generated to test the requirement for IL-4 in vivo. In the mutant mice T and B cell development was normal, but the serum levels of IgG1 and IgE were strongly reduced. The IgG1 dominance in a T cell-dependent immune response was lost, and IgE was not detectable upon nematode infection. Thus, some but not all of the in vitro properties of IL-4 are critical for the physiology of the immune system in vivo.read more
Citations
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Expression profiling in transgenic FVB/N embryonic stem cells overexpressing STAT3.
Paolo Cinelli,Elisa A. Casanova,Syndi Uhlig,Priska Lochmatter,Takahiko Matsuda,Takashi Yokota,Takashi Yokota,Thomas Rülicke,Thomas Rülicke,Birgit Ledermann,Birgit Ledermann,Kurt Bürki +11 more
TL;DR: Two genes, Pem/Rhox5 and Pramel7, when overexpressed in ES cells are able to maintain the embryonic stem cells in a pluripotent state in a LIF independent manner as STAT3 or Nanog.
Journal ArticleDOI
Glycoprotein Hyposialylation Gives Rise to a Nephrotic-Like Syndrome That Is Prevented by Sialic Acid Administration in GNE V572L Point-Mutant Mice
Mitutoshi Ito,Kazushi Sugihara,Tomoya Asaka,Tadashi Toyama,Toru Yoshihara,Kengo Furuichi,Takashi Wada,Masahide Asano +7 more
TL;DR: It is suggested that the nephrotic-like syndrome observed in these mutant mice resulted from impaired glomerular filtration due to the hyposialylation of podocyte glycoproteins, including podocalyxin.
Journal ArticleDOI
The Prokaryotic β-Recombinase Catalyzes Site-specific Recombination in Mammalian Cells
TL;DR: This work provides the first evidence that the prokaryotic site-specific recombinase (β-recombinase), which catalyzes only intramolecular recombination, is active in eukaryotic environments.
Journal ArticleDOI
Targeting the mouse genome: a compedium of knockouts
TL;DR: The database includes omissions and errors in the entries up to the end of 1994 that had come to light as of 1st September 1995 and will be updated as an ongoing process.
Journal ArticleDOI
Comparison of cytokine secretion profiles provoked in mice by glutaraldehyde and formaldehyde.
TL;DR: Topical exposure of mice to chemical contact allergens such as 2,4‐dinitrochlorobenzene (DNCB) or respiratory allergenssuch as trimellitic anhydride (TMA) induces characteristic cytokine secretion profiles consistent with the selective activation of T helper 1 and TH2‐type cells, respectively.
References
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Journal ArticleDOI
TH1 and TH2 cells: different patterns of lymphokine secretion lead to different functional properties.
Tim R. Mosmann,R L Coffman +1 more
TL;DR: Two types of cloned helper T cells are described, defined primarily by differences in the pattern of lymphokines ynthesized, and the different functions of the two types of cells and their lymphokine synthesis are discussed.
Journal ArticleDOI
Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells.
Kirk R. Thomas,Mario R. Capecchi +1 more
TL;DR: This work mutated, by gene targeting, the endogenous hypoxanthine phosphoribosyl transferase (HPRT) gene in mouse embryo-derived stem (ES) cells and compared the gene-targeting efficiencies of two classes of neor-Hprt recombinant vectors.
Journal ArticleDOI
Altering the genome by homologous recombination.
TL;DR: The current status of gene targeting with particular emphasis on germ line modification of the mouse genome is discussed, and the different methods so far employed to identify those rare embryonic stem cells in which the desired targeting event has occurred are described.
Journal ArticleDOI
Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells
Rachel Williams,D J Hilton,S Pease,Tracy A. Willson,Colin L. Stewart,David P. Gearing,Erwin F. Wagner,D. Metcalf,Nicos A. Nicola,Nicholas M. Gough +9 more
TL;DR: In this paper, a recombinant myeloid leukaemia inhibitory factor (LIF) was used to replace DIA in the maintenance of totipotent ES cell lines that retain the potential to form chimaeric mice.
Journal ArticleDOI
Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens.
TL;DR: This paper used hybridoma monoclonal antibodies obtained after immunization of mice with rat cells to study rat cell-surface antigens present on subpopulations of rat lymphocytes.
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