Journal ArticleDOI
Generation and analysis of interleukin-4 deficient mice.
TLDR
Some but not all of the in vitro properties of IL-4 are critical for the physiology of the immune system in vivo, but the serum levels of IgG1 and IgE are strongly reduced.Abstract:
Interleukin-4 (IL-4) promotes the growth and differentiation of many hematopoietic cells in vitro; in particular, it directs the immunoglobulin (Ig) class switch to IgG1 and IgE. Mice homozygous for a mutation that inactivates the IL-4 gene were generated to test the requirement for IL-4 in vivo. In the mutant mice T and B cell development was normal, but the serum levels of IgG1 and IgE were strongly reduced. The IgG1 dominance in a T cell-dependent immune response was lost, and IgE was not detectable upon nematode infection. Thus, some but not all of the in vitro properties of IL-4 are critical for the physiology of the immune system in vivo.read more
Citations
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Reduced COVID-19 Vaccine Response in Patients Treated with Biologic Therapies for Asthma
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Reduced COVID-19 Vaccine Response in Patients Treated with Biologic Therapies for Asthma
Martin Runnstrom,Andrea Morrison-Porter,M Rekha Ravindran,Hannah Quehl,Richard P. Ramonell,Matthew C. Woodruff,Rahulkumar Patel,Caroline Kim,Natalie S. Haddad,F. Eun-Hyung Lee +9 more
TL;DR: Ehrmann et al. as discussed by the authors performed a systematic review and meta-analysis of non-randomized cohort studies to evaluate the effect of timing of intubation on clinical outcomes of critically ill patients with COVID-19 acute hypoxaemic respiratory failure.
Journal ArticleDOI
Kinoids: A family of immunogens for active anticytokine immunotherapy applied to autoimmune diseases and cancer
Bernard Bizzini,Béatrice Drouet,Daniel Zagury,Marc Abitbol,Arsène Burny,Marie-Christophe Boissier +5 more
TL;DR: The discovery of a family of immunogens, the kinoids, consisting of inactivated cytokine derivatives, has led some to propose them for active immunotherapy as an alternative to passive immunotherapy.
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Modulation of cell-bound and soluble CD23, spontaneous and ongoing IgE synthesis of human peripheral blood mononuclear cells by soluble IL-4 receptors and the partial antagonistic IL-4 mutant protein IL-4 (Y124D).
B König,A Fischer,W König +2 more
TL;DR: It is demonstrated that sIL-4R and IL-4(Y124D) are suppressive for the primary IgE synthesis induced by IL- 4, and a differential function for IL-3 in the early and late phase of PBMC IgE production is suggested.
Journal ArticleDOI
TH1-promoting DNA immunization against allergens modulates the ratio of IgG1/IgG2a but does not affect the anaphylactic potential of IgG1 antibodies: No evidence for the synthesis of nonanaphylactic IgG1
TL;DR: The results reveal no evidence for the production of nonanaphylactic IgG1, and indicate that the development of non-IgE-mediated anaphylaxis does not require IL-4 receptor signaling.
References
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TH1 and TH2 cells: different patterns of lymphokine secretion lead to different functional properties.
Tim R. Mosmann,R L Coffman +1 more
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TL;DR: The current status of gene targeting with particular emphasis on germ line modification of the mouse genome is discussed, and the different methods so far employed to identify those rare embryonic stem cells in which the desired targeting event has occurred are described.
Journal ArticleDOI
Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells
Rachel Williams,D J Hilton,S Pease,Tracy A. Willson,Colin L. Stewart,David P. Gearing,Erwin F. Wagner,D. Metcalf,Nicos A. Nicola,Nicholas M. Gough +9 more
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