Simple Combinations of Lineage-Determining Transcription Factors Prime cis-Regulatory Elements Required for Macrophage and B Cell Identities
Sven Heinz,Christopher Benner,Nathanael J. Spann,Eric Bertolino,Yin C. Lin,Peter Laslo,Jason X. Cheng,Cornelis Murre,Harinder Singh,Harinder Singh,Christopher K. Glass +10 more
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TLDR
It is demonstrated in macrophages and B cells that collaborative interactions of the common factor PU.1 with small sets of macrophage- or B cell lineage-determining transcription factors establish cell-specific binding sites that are associated with the majority of promoter-distal H3K4me1-marked genomic regions.About:
This article is published in Molecular Cell.The article was published on 2010-05-28 and is currently open access. It has received 9620 citations till now. The article focuses on the topics: Pioneer factor & General transcription factor.read more
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Allergic inflammatory memory in human respiratory epithelial progenitor cells.
Jose Ordovas-Montanes,Daniel F. Dwyer,Daniel F. Dwyer,Sarah K. Nyquist,Kathleen M. Buchheit,Kathleen M. Buchheit,Marko Vukovic,Chaarushena Deb,Marc H. Wadsworth,Travis K. Hughes,Samuel W. Kazer,Eri Yoshimoto,Eri Yoshimoto,Katherine N. Cahill,Katherine N. Cahill,Neil Bhattacharyya,Neil Bhattacharyya,Howard R. Katz,Howard R. Katz,Bonnie Berger,Tanya M. Laidlaw,Tanya M. Laidlaw,Joshua A. Boyce,Joshua A. Boyce,Nora A. Barrett,Nora A. Barrett,Alex K. Shalek +26 more
TL;DR: It is found that reduced epithelial diversity stemming from functional shifts in basal cells is a key characteristic of type 2 immune-mediated barrier tissue dysfunction and may contribute to the persistence of human disease by serving as repositories for allergic memories.
Journal ArticleDOI
The role of the local environment and epigenetics in shaping macrophage identity and their effect on tissue homeostasis
TL;DR: It is argued that epigenetic regulation of macrophages is determined by lineage- and tissue-specific transcription factors controlled by the built-in programming of myeloid development in combination with signaling from the tissue environment.
Journal ArticleDOI
Transcriptional profiling of mouse B cell terminal differentiation defines a signature for antibody-secreting plasma cells
Wei Shi,Yang Liao,Simon N. Willis,Nadine Taubenheim,Michael Inouye,David M. Tarlinton,Gordon K. Smyth,Philip D. Hodgkin,Stephen L. Nutt,Lynn M. Corcoran +9 more
TL;DR: The transcriptomes of many mature B cell populations and stages of plasma cell differentiation in mice are defined and a molecular signature of ASCs is provided that highlights the stark transcriptional divide between B cells and plasma cells and enables the demarcation ofASCs on the basis of location and maturity.
Journal ArticleDOI
Requirement for the histone deacetylase Hdac3 for the inflammatory gene expression program in macrophages
Xuefen Chen,Iros Barozzi,Alberto Termanini,Elena Prosperini,Antonio Recchiuti,Jesmond Dalli,Flore Mietton,Gianluca Matteoli,Scott W. Hiebert,Gioacchino Natoli +9 more
TL;DR: Using an integrated genomic approach, it is found that Hdac3-deficient macrophages were unable to activate almost half of the inflammatory gene expression program when stimulated with LPS, indicating a central role for HdAc3 in inflammation and may have relevance for the use of selective HdAC inhibitors as antiinflammatory agents.
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The Genetics of Transcription Factor DNA Binding Variation
TL;DR: The findings that led to this important paradigm shift are summarized and proposed mechanisms for local, proximal, or distal genetic variation-driven variable TF-DNA binding are reviewed.
References
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RAG-1-deficient mice have no mature B and T lymphocytes
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TL;DR: This study uses chromatin immunoprecipitation coupled with ultra-high-throughput DNA sequencing to map the locations of TF-binding sites and identifies important features of the transcriptional regulatory networks that define ES-cell identity.
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Genome-scale DNA methylation maps of pluripotent and differentiated cells
Alexander Meissner,Tarjei S. Mikkelsen,Tarjei S. Mikkelsen,Hongcang Gu,Marius Wernig,Jacob H. Hanna,Andrey Sivachenko,Xiaolan Zhang,Bradley E. Bernstein,Bradley E. Bernstein,Chad Nusbaum,David B. Jaffe,Andreas Gnirke,Rudolf Jaenisch,Eric S. Lander +14 more
TL;DR: Low-throughput reduced representation bisulphite sequencing is established as a powerful technology for epigenetic profiling of cell populations relevant to developmental biology, cancer and regenerative medicine.