Simple Combinations of Lineage-Determining Transcription Factors Prime cis-Regulatory Elements Required for Macrophage and B Cell Identities
Sven Heinz,Christopher Benner,Nathanael J. Spann,Eric Bertolino,Yin C. Lin,Peter Laslo,Jason X. Cheng,Cornelis Murre,Harinder Singh,Harinder Singh,Christopher K. Glass +10 more
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TLDR
It is demonstrated in macrophages and B cells that collaborative interactions of the common factor PU.1 with small sets of macrophage- or B cell lineage-determining transcription factors establish cell-specific binding sites that are associated with the majority of promoter-distal H3K4me1-marked genomic regions.About:
This article is published in Molecular Cell.The article was published on 2010-05-28 and is currently open access. It has received 9620 citations till now. The article focuses on the topics: Pioneer factor & General transcription factor.read more
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Natural regulatory mutations elevate the fetal globin gene via disruption of BCL11A or ZBTB7A binding
Gabriella E. Martyn,Beeke Wienert,Lu Yang,Manan Shah,Laura J. Norton,Jon Burdach,Ryo Kurita,Yukio Nakamura,Richard C. M. Pearson,Alister P. W. Funnell,Kate G. R. Quinlan,Merlin Crossley +11 more
TL;DR: It is found that the major fetal globin gene repressors BCL11A and ZBTB7A directly bound to the sites at –115 and –200 bp, respectively, and introduced of naturally occurring HPFH-associated mutations into erythroid cells by CRISPR–Cas9 disrupted repressor binding and raised γ-globin gene expression.
Journal ArticleDOI
Pathological priming causes developmental gene network heterochronicity in autistic subject-derived neurons
Simon T. Schafer,Apuã C. M. Paquola,Apuã C. M. Paquola,Shani Stern,David Gosselin,Manching Ku,Manching Ku,Monique Pena,Thomas J.M. Kuret,Marvin Liyanage,Abed AlFatah Mansour,Baptiste N. Jaeger,Baptiste N. Jaeger,Maria C. Marchetto,Christopher K. Glass,Jerome Mertens,Jerome Mertens,Fred H. Gage +17 more
TL;DR: Using a dynamic time-course approach to model developmental trajectories in autism spectrum disorder, Schafer et al. identified aberrant gene network dynamics as a part of an ongoing process that is primed early in development.
Journal ArticleDOI
MYB-QKI rearrangements in angiocentric glioma drive tumorigenicity through a tripartite mechanism.
Pratiti Bandopadhayay,Pratiti Bandopadhayay,Lori A. Ramkissoon,Payal Jain,Guillaume Bergthold,Guillaume Bergthold,Jeremiah Wala,Jeremiah Wala,Rhamy Zeid,Steven E. Schumacher,Steven E. Schumacher,Laura M. Urbanski,Ryan O’Rourke,Ryan O’Rourke,William J. Gibson,William J. Gibson,Kristine Pelton,Shakti Ramkissoon,Harry J. Han,Yuankun Zhu,Namrata Choudhari,Amanda Silva,Amanda Silva,Katie Boucher,Rosemary E. Henn,Yun Jee Kang,David S. Knoff,Brenton R. Paolella,Brenton R. Paolella,Adrianne Gladden-Young,Pascale Varlet,Mélanie Pagès,Peleg M. Horowitz,Alexander J. Federation,Hayley Malkin,Adam Tracy,Sara Seepo,Matthew D. Ducar,Paul Van Hummelen,Mariarita Santi,Anna Maria Buccoliero,Mirko Scagnet,Daniel C. Bowers,Caterina Giannini,Stéphanie Puget,Cynthia Hawkins,Uri Tabori,Almos Klekner,László Bognár,Peter C. Burger,Charles G. Eberhart,Fausto J. Rodriguez,D. Ashley Hill,Sabine Mueller,Daphne A. Haas-Kogan,Daphne A. Haas-Kogan,Joanna J. Phillips,Sandro Santagata,Charles D. Stiles,James E. Bradner,James E. Bradner,Nada Jabado,Alon Goren,Jacques Grill,Azra H. Ligon,Liliana Goumnerova,Angela J. Waanders,Phillip B. Storm,Mark W. Kieran,Keith L. Ligon,Keith L. Ligon,Rameen Beroukhim,Rameen Beroukhim,Adam C. Resnick +73 more
TL;DR: This work has identified MYB-QKI fusions as a specific and single candidate driver event in angiocentric gliomas and represents the first example of a single driver rearrangement simultaneously transforming cells via three genetic and epigenetic mechanisms in a tumor.
Journal ArticleDOI
The Forkhead Transcription Factor FOXM1 Controls Cell Cycle-Dependent Gene Expression through an Atypical Chromatin Binding Mechanism
Xi Chen,Gerd A. Müller,Marianne Quaas,Martin Fischer,Namshik Han,Benjamin Stutchbury,Andrew D. Sharrocks,Kurt Engeland +7 more
TL;DR: A novel and unexpected mode of chromatin binding of a FOX transcription factor that allows it to specifically control cell cycle-dependent gene expression is uncovered.
Journal ArticleDOI
Fat Mass and Obesity-associated (FTO) Protein Regulates Adult Neurogenesis.
Liping Li,Liping Li,Liqun Zang,Liqun Zang,Feiran Zhang,Junchen Chen,Junchen Chen,Hui Shen,Hui Shen,Liqi Shu,Liqi Shu,Feng Liang,Feng Liang,Chunyue Feng,Deng Chen,Huikang Tao,Tianlei Xu,Ziyi Li,Yunhee Kang,Hao Wu,Lichun Tang,Pumin Zhang,Peng Jin,Qiang Shu,Xuekun Li,Xuekun Li +25 more
TL;DR: It is shown that FTO is expressed in adult neural stem cells and neurons and displays dynamic expression during postnatal neurodevelopment and this results suggest FTO plays important roles in neurogenesis, as well as in learning and memory.
References
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Artem Barski,Suresh Cuddapah,Kairong Cui,Tae-Young Roh,Dustin E. Schones,Zhibin Wang,Gang Wei,Iouri Chepelev,Keji Zhao +8 more
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RAG-1-deficient mice have no mature B and T lymphocytes
Peter Mombaerts,John Iacomini,Randall S. Johnson,Karl Herrup,Susumu Tonegawa,Virginia E. Papaioannou +5 more
TL;DR: The introduction of a mutation in RAG-1 into the germline of mice via gene targeting in embryonic stem cells is described and it is shown that this mutation either activates or catalyzes the V(D)J recombination reaction of immunoglobulin and T cell receptor genes.
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Integration of External Signaling Pathways with the Core Transcriptional Network in Embryonic Stem Cells
Xi Chen,Han Xu,Ping Yuan,Fang Fang,Fang Fang,Mikael Huss,Vinsensius B. Vega,Eleanor Wong,Yuriy L. Orlov,Weiwei Zhang,Weiwei Zhang,Jianming Jiang,Jianming Jiang,Yuin-Han Loh,Yuin-Han Loh,Hock Chuan Yeo,Zhen Xuan Yeo,Vipin Narang,Kunde R Govindarajan,Bernard Leong,Atif Shahab,Yijun Ruan,Guillaume Bourque,Wing-Kin Sung,Neil D. Clarke,Chia-Lin Wei,Huck-Hui Ng,Huck-Hui Ng +27 more
TL;DR: This study uses chromatin immunoprecipitation coupled with ultra-high-throughput DNA sequencing to map the locations of TF-binding sites and identifies important features of the transcriptional regulatory networks that define ES-cell identity.
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Genome-scale DNA methylation maps of pluripotent and differentiated cells
Alexander Meissner,Tarjei S. Mikkelsen,Tarjei S. Mikkelsen,Hongcang Gu,Marius Wernig,Jacob H. Hanna,Andrey Sivachenko,Xiaolan Zhang,Bradley E. Bernstein,Bradley E. Bernstein,Chad Nusbaum,David B. Jaffe,Andreas Gnirke,Rudolf Jaenisch,Eric S. Lander +14 more
TL;DR: Low-throughput reduced representation bisulphite sequencing is established as a powerful technology for epigenetic profiling of cell populations relevant to developmental biology, cancer and regenerative medicine.