Simple Combinations of Lineage-Determining Transcription Factors Prime cis-Regulatory Elements Required for Macrophage and B Cell Identities
Sven Heinz,Christopher Benner,Nathanael J. Spann,Eric Bertolino,Yin C. Lin,Peter Laslo,Jason X. Cheng,Cornelis Murre,Harinder Singh,Harinder Singh,Christopher K. Glass +10 more
Reads0
Chats0
TLDR
It is demonstrated in macrophages and B cells that collaborative interactions of the common factor PU.1 with small sets of macrophage- or B cell lineage-determining transcription factors establish cell-specific binding sites that are associated with the majority of promoter-distal H3K4me1-marked genomic regions.About:
This article is published in Molecular Cell.The article was published on 2010-05-28 and is currently open access. It has received 9620 citations till now. The article focuses on the topics: Pioneer factor & General transcription factor.read more
Citations
More filters
Journal ArticleDOI
Xist Deletional Analysis Reveals an Interdependency between Xist RNA and Polycomb Complexes for Spreading along the Inactive X.
David Colognori,David Colognori,Hongjae Sunwoo,Hongjae Sunwoo,Andrea J. Kriz,Andrea J. Kriz,Chen-Yu Wang,Chen-Yu Wang,Jeannie T. Lee,Jeannie T. Lee +9 more
TL;DR: Repeat B is a multifunctional element that integrates interdependent Xist/Polycomb spreading, silencing, and changes in chromosome architecture and suggests a positive feedback mechanism between RNA initiator and protein effectors.
Journal ArticleDOI
Precursors for Nonlymphoid-Tissue Treg Cells Reside in Secondary Lymphoid Organs and Are Programmed by the Transcription Factor BATF
Michael Delacher,Michael Delacher,Charles D. Imbusch,Charles D. Imbusch,Agnes Hotz-Wagenblatt,Jan-Philipp Mallm,Katharina Bauer,Malte Simon,Malte Simon,Dania Riegel,André F. Rendeiro,Sebastian Bittner,Lieke Sanderink,Asmita Pant,Lisa Schmidleithner,Kathrin L. Braband,Bernd Echtenachter,Alexander Fischer,Valentina Giunchiglia,Valentina Giunchiglia,Petra Hoffmann,Matthias Edinger,Christoph Bock,Michael Rehli,Benedikt Brors,Christian Schmidl,Markus Feuerer,Markus Feuerer +27 more
TL;DR: This work identified two precursor stages of interleukin 33 (IL-33) receptor ST2-expressing nonlymphoid tissue Treg cells, which resided in the spleen and lymph nodes and validated the transcription factor Batf as the driver of the molecular tissue program in the precursors.
Journal ArticleDOI
GRHL2-miR-200-ZEB1 maintains the epithelial status of ovarian cancer through transcriptional regulation and histone modification
Vin Yee Chung,Tuan Zea Tan,Ming Tan,Meng Kang Wong,K.T. Kuay,Zhe Yang,Jieru Ye,Julius Muller,Cheryl M. Koh,Ernesto Guccione,Jean Paul Thiery,Jean Paul Thiery,Ruby Yun-Ju Huang +12 more
TL;DR: It is reported that a transcription factor—Grainyhead-like 2 (GRHL2) maintains the epithelial phenotype and support GRHL2 as a pivotal gatekeeper of EMT in EOC via miR-200-ZEB1.
Journal ArticleDOI
A chromatin integration labelling method enables epigenomic profiling with lower input.
Akihito Harada,Kazumitsu Maehara,Tetsuya Handa,Yasuhiro Arimura,Jumpei Nogami,Yoko Hayashi-Takanaka,Katsuhiko Shirahige,Hitoshi Kurumizaka,Hitoshi Kurumizaka,Hiroshi Kimura,Yasuyuki Ohkawa +10 more
TL;DR: An immunoprecipitation-free epigenomic profiling method named chromatin integration labelling (ChIL), which enables the amplification of genomic sequences closely associated with the target molecules before cell lysis and reliably detected the distributions of histone modifications and DNA-binding factors in 100–1,000 cells.
Journal ArticleDOI
Genomic and Epigenomic Features of Primary and Recurrent Hepatocellular Carcinomas
Xiaofan Ding,Mian He,Anthony W.H. Chan,Qi Xiu Song,Siu Ching Sze,Hui Chen,Matthew K.H. Man,Kwan Man,Stephen L. Chan,Paul B.S. Lai,Xin Wang,Nathalie Wong +11 more
TL;DR: A systematic evaluation of intra- and inter-tumor genetic heterogeneity in HCC samples was performed and genetic and epigenetic changes that associate with tumor progression and recurrence were identified.
References
More filters
Journal ArticleDOI
High-resolution profiling of histone methylations in the human genome.
Artem Barski,Suresh Cuddapah,Kairong Cui,Tae-Young Roh,Dustin E. Schones,Zhibin Wang,Gang Wei,Iouri Chepelev,Keji Zhao +8 more
TL;DR: High-resolution maps for the genome-wide distribution of 20 histone lysine and arginine methylations as well as histone variant H2A.Z, RNA polymerase II, and the insulator binding protein CTCF across the human genome using the Solexa 1G sequencing technology are generated.
Journal ArticleDOI
Genome-wide maps of chromatin state in pluripotent and lineage-committed cells
Tarjei S. Mikkelsen,Manching Ku,Manching Ku,David B. Jaffe,Biju Issac,Biju Issac,Erez Lieberman Aiden,Erez Lieberman Aiden,Georgia Giannoukos,Pablo Alvarez,William Brockman,Tae Kyung Kim,Richard Koche,Richard Koche,Richard Koche,William Lee,Eric M. Mendenhall,Eric M. Mendenhall,Aisling O'Donovan,Aviva Presser,Carsten Russ,Xiaohui Xie,Alexander Meissner,Marius Wernig,Rudolf Jaenisch,Chad Nusbaum,Eric S. Lander,Eric S. Lander,Bradley E. Bernstein,Bradley E. Bernstein +29 more
TL;DR: The application of single-molecule-based sequencing technology for high-throughput profiling of histone modifications in mammalian cells is reported and it is shown that chromatin state can be read in an allele-specific manner by using single nucleotide polymorphisms.
Journal ArticleDOI
RAG-1-deficient mice have no mature B and T lymphocytes
Peter Mombaerts,John Iacomini,Randall S. Johnson,Karl Herrup,Susumu Tonegawa,Virginia E. Papaioannou +5 more
TL;DR: The introduction of a mutation in RAG-1 into the germline of mice via gene targeting in embryonic stem cells is described and it is shown that this mutation either activates or catalyzes the V(D)J recombination reaction of immunoglobulin and T cell receptor genes.
Journal ArticleDOI
Integration of External Signaling Pathways with the Core Transcriptional Network in Embryonic Stem Cells
Xi Chen,Han Xu,Ping Yuan,Fang Fang,Fang Fang,Mikael Huss,Vinsensius B. Vega,Eleanor Wong,Yuriy L. Orlov,Weiwei Zhang,Weiwei Zhang,Jianming Jiang,Jianming Jiang,Yuin-Han Loh,Yuin-Han Loh,Hock Chuan Yeo,Zhen Xuan Yeo,Vipin Narang,Kunde R Govindarajan,Bernard Leong,Atif Shahab,Yijun Ruan,Guillaume Bourque,Wing-Kin Sung,Neil D. Clarke,Chia-Lin Wei,Huck-Hui Ng,Huck-Hui Ng +27 more
TL;DR: This study uses chromatin immunoprecipitation coupled with ultra-high-throughput DNA sequencing to map the locations of TF-binding sites and identifies important features of the transcriptional regulatory networks that define ES-cell identity.
Journal ArticleDOI
Genome-scale DNA methylation maps of pluripotent and differentiated cells
Alexander Meissner,Tarjei S. Mikkelsen,Tarjei S. Mikkelsen,Hongcang Gu,Marius Wernig,Jacob H. Hanna,Andrey Sivachenko,Xiaolan Zhang,Bradley E. Bernstein,Bradley E. Bernstein,Chad Nusbaum,David B. Jaffe,Andreas Gnirke,Rudolf Jaenisch,Eric S. Lander +14 more
TL;DR: Low-throughput reduced representation bisulphite sequencing is established as a powerful technology for epigenetic profiling of cell populations relevant to developmental biology, cancer and regenerative medicine.