Simple Combinations of Lineage-Determining Transcription Factors Prime cis-Regulatory Elements Required for Macrophage and B Cell Identities
Sven Heinz,Christopher Benner,Nathanael J. Spann,Eric Bertolino,Yin C. Lin,Peter Laslo,Jason X. Cheng,Cornelis Murre,Harinder Singh,Harinder Singh,Christopher K. Glass +10 more
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TLDR
It is demonstrated in macrophages and B cells that collaborative interactions of the common factor PU.1 with small sets of macrophage- or B cell lineage-determining transcription factors establish cell-specific binding sites that are associated with the majority of promoter-distal H3K4me1-marked genomic regions.About:
This article is published in Molecular Cell.The article was published on 2010-05-28 and is currently open access. It has received 9620 citations till now. The article focuses on the topics: Pioneer factor & General transcription factor.read more
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Lamins Organize the Global Three-Dimensional Genome from the Nuclear Periphery
Xiaobin Zheng,Jiabiao Hu,Sibiao Yue,Lidya Kristiani,Miri Kim,Michael E.G. Sauria,James Taylor,Youngjo Kim,Yixian Zheng +8 more
TL;DR: It is revealed that lamin loss causes expansion or detachment of specific LADs in mouse ESCs, and a significant correlation between transcription changes and the interaction changes of active and inactive chromatin domains is found.
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Epstein-Barr Virus Oncoprotein Super-enhancers Control B Cell Growth
Hufeng Zhou,Hufeng Zhou,Stefanie C.S. Schmidt,Stefanie C.S. Schmidt,Sizun Jiang,Sizun Jiang,Bradford Willox,Katharina Bernhardt,Katharina Bernhardt,Jun Liang,Jun Liang,Eric Johannsen,Peter V. Kharchenko,Benjamin E. Gewurz,Benjamin E. Gewurz,Elliott Kieff,Elliott Kieff,Bo Zhao,Bo Zhao +18 more
TL;DR: In insight into mechanisms of EBV-induced lymphoproliferation and potential therapeutic interventions, EBV super-enhancer-associated genes included the MYC and BCL2 oncogenes, which enable LCL proliferation and survival are provided.
Journal ArticleDOI
Erk1/2 Activity Promotes Chromatin Features and RNAPII Phosphorylation at Developmental Promoters in Mouse ESCs
TL;DR: Findings underscore a key role for Erk1/2 activation in promoting the primed status of developmental genes in mouse ES cells and suggest that the transcription complex at developmental genes is different than the complexes formed at other genes, offering alternative pathways of regulation.
Journal ArticleDOI
Chromatin Accessibility Landscape in Human Early Embryos and Its Association with Evolution.
Lei Gao,Keliang Wu,Zhenbo Liu,Xuelong Yao,Xuelong Yao,Shenli Yuan,Shenli Yuan,Wenrong Tao,Lizhi Yi,Lizhi Yi,Guanling Yu,Zhenzhen Hou,Dongdong Fan,Yong Tian,Jianqiao Liu,Zi-Jiang Chen,Jiang Liu,Jiang Liu +17 more
TL;DR: DNase I hypersensitive site (DHS) sequencing data provide an evolutionary developmental view for understanding the regulation of gene and transposon expression and show that human active transposons SVA and HERV-K harbor DHSs and are highly expressed in early embryos, but not in differentiated tissues.
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Single-Cell RNA Sequencing of the T Helper Cell Response to House Dust Mites Defines a Distinct Gene Expression Signature in Airway Th2 Cells.
Christopher A. Tibbitt,Julian M. Stark,Liesbet Martens,Junjie Ma,Jeff E. Mold,Kim Deswarte,Ganna Oliynyk,Xiaogang Feng,Bart N. Lambrecht,Pieter De Bleser,Susanne Nylén,Hamida Hammad,Marie Henriksson,Yvan Saeys,Jonathan M. Coquet +14 more
TL;DR: Single-cell RNA sequencing is performed of T helper cells from lymph node, lung, and airways in the house dust mite model of allergic airway disease to provide insight into the differentiation of pathogenic Th2 cells in the context of allergy.
References
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Genome-scale DNA methylation maps of pluripotent and differentiated cells
Alexander Meissner,Tarjei S. Mikkelsen,Tarjei S. Mikkelsen,Hongcang Gu,Marius Wernig,Jacob H. Hanna,Andrey Sivachenko,Xiaolan Zhang,Bradley E. Bernstein,Bradley E. Bernstein,Chad Nusbaum,David B. Jaffe,Andreas Gnirke,Rudolf Jaenisch,Eric S. Lander +14 more
TL;DR: Low-throughput reduced representation bisulphite sequencing is established as a powerful technology for epigenetic profiling of cell populations relevant to developmental biology, cancer and regenerative medicine.