Simple Combinations of Lineage-Determining Transcription Factors Prime cis-Regulatory Elements Required for Macrophage and B Cell Identities
Sven Heinz,Christopher Benner,Nathanael J. Spann,Eric Bertolino,Yin C. Lin,Peter Laslo,Jason X. Cheng,Cornelis Murre,Harinder Singh,Harinder Singh,Christopher K. Glass +10 more
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TLDR
It is demonstrated in macrophages and B cells that collaborative interactions of the common factor PU.1 with small sets of macrophage- or B cell lineage-determining transcription factors establish cell-specific binding sites that are associated with the majority of promoter-distal H3K4me1-marked genomic regions.About:
This article is published in Molecular Cell.The article was published on 2010-05-28 and is currently open access. It has received 9620 citations till now. The article focuses on the topics: Pioneer factor & General transcription factor.read more
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Matrix stiffness induces a tumorigenic phenotype in mammary epithelium through changes in chromatin accessibility.
Ryan S. Stowers,Anna Shcherbina,Johnny Israeli,Joshua J. Gruber,Julie Chang,Sungmin Nam,Atefeh Rabiee,Mary N. Teruel,Michael Snyder,Anshul Kundaje,Ovijit Chaudhuri +10 more
TL;DR: 3D model of breast cancer shows that a stiff extracellular matrix promotes a tumorigenic phenotype through broad changes in chromatin accessibility and in the activity of histone deacetylases and the transcription factor Sp1, and reveals that chromatin state is a critical mediator of mechanotransduction.
Journal ArticleDOI
The transcription factors IRF8 and PU.1 negatively regulate plasma cell differentiation
Sebastian Carotta,Sebastian Carotta,Simon N. Willis,Simon N. Willis,Jhagvaral Hasbold,Jhagvaral Hasbold,Michael Inouye,Michael Inouye,Swee Heng Milon Pang,Swee Heng Milon Pang,Dianne Emslie,Amanda Light,Michael Chopin,Michael Chopin,Wei Shi,Wei Shi,Hongsheng Wang,Herbert C. Morse,David M. Tarlinton,David M. Tarlinton,Lynn M. Corcoran,Lynn M. Corcoran,Philip D. Hodgkin,Philip D. Hodgkin,Stephen L. Nutt,Stephen L. Nutt +25 more
TL;DR: It is shown that the interaction between IRF8 and PU.1 controls the propensity of B cells to undergo class-switch recombination and plasma cell differentiation by concurrently promoting the expression of BCL6 and PAX5 and repressing AID and BLIMP-1.
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Type 2 Diabetes Variants Disrupt Function of SLC16A11 through Two Distinct Mechanisms
Victor Rusu,Eitan Hoch,Josep M. Mercader,Danielle Tenen,Melissa Gymrek,Christina R. Hartigan,Michael DeRan,Marcin von Grotthuss,Pierre Fontanillas,Alexandra Spooner,Gaelen Guzman,Amy Deik,Kerry A. Pierce,Courtney Dennis,Clary B. Clish,Steven A. Carr,Bridget K. Wagner,Monica Schenone,Maggie C.Y. Ng,Brian H. Chen,Federico Centeno-Cruz,Carlos Zerrweck,Lorena Orozco,David Altshuler,Stuart L. Schreiber,Jose C. Florez,Suzanne B.R. Jacobs,Eric S. Lander +27 more
TL;DR: It is demonstrated that SLC16A11 is a proton-coupled monocarboxylate transporter and that genetic perturbation of SLC15A11 induces changes in fatty acid and lipid metabolism that are associated with increased T2D risk, and suggested that increasing SLC 16A11 function could be therapeutically beneficial for T1D.
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Transcriptome-wide N⁶-methyladenosine profiling of rice callus and leaf reveals the presence of tissue-specific competitors involved in selective mRNA modification.
TL;DR: A transcriptome-wide m6A modification profiling effort for rice transcriptomes of differentiated callus and leaf yields 8,138 and 14,253 m6a-modified genes, respectively, which provide a resource for plant RNA epitranscriptomic studies and further enlarge the knowledge on the function of RNA m 6A modification.
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Wounding induces dedifferentiation of epidermal Gata6 + cells and acquisition of stem cell properties
Giacomo Donati,Emanuel Rognoni,Toru Hiratsuka,Kifayathullah Liakath-Ali,Esther Hoste,Esther Hoste,Gozde Kar,Melis Kayikci,Roslin Russell,Kai Kretzschmar,Kai Kretzschmar,Klaas W. Mulder,Sarah A. Teichmann,Fiona M. Watt +13 more
TL;DR: The data not only demonstrate that the structural and functional complexity of the junctional zone is regulated by Gata6, but also reveal that dedifferentiation is a previously unrecognized property of post-mitotic, terminally differentiated cells that have lost contact with the basement membrane.
References
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Artem Barski,Suresh Cuddapah,Kairong Cui,Tae-Young Roh,Dustin E. Schones,Zhibin Wang,Gang Wei,Iouri Chepelev,Keji Zhao +8 more
TL;DR: High-resolution maps for the genome-wide distribution of 20 histone lysine and arginine methylations as well as histone variant H2A.Z, RNA polymerase II, and the insulator binding protein CTCF across the human genome using the Solexa 1G sequencing technology are generated.
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RAG-1-deficient mice have no mature B and T lymphocytes
Peter Mombaerts,John Iacomini,Randall S. Johnson,Karl Herrup,Susumu Tonegawa,Virginia E. Papaioannou +5 more
TL;DR: The introduction of a mutation in RAG-1 into the germline of mice via gene targeting in embryonic stem cells is described and it is shown that this mutation either activates or catalyzes the V(D)J recombination reaction of immunoglobulin and T cell receptor genes.
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Integration of External Signaling Pathways with the Core Transcriptional Network in Embryonic Stem Cells
Xi Chen,Han Xu,Ping Yuan,Fang Fang,Fang Fang,Mikael Huss,Vinsensius B. Vega,Eleanor Wong,Yuriy L. Orlov,Weiwei Zhang,Weiwei Zhang,Jianming Jiang,Jianming Jiang,Yuin-Han Loh,Yuin-Han Loh,Hock Chuan Yeo,Zhen Xuan Yeo,Vipin Narang,Kunde R Govindarajan,Bernard Leong,Atif Shahab,Yijun Ruan,Guillaume Bourque,Wing-Kin Sung,Neil D. Clarke,Chia-Lin Wei,Huck-Hui Ng,Huck-Hui Ng +27 more
TL;DR: This study uses chromatin immunoprecipitation coupled with ultra-high-throughput DNA sequencing to map the locations of TF-binding sites and identifies important features of the transcriptional regulatory networks that define ES-cell identity.
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Genome-scale DNA methylation maps of pluripotent and differentiated cells
Alexander Meissner,Tarjei S. Mikkelsen,Tarjei S. Mikkelsen,Hongcang Gu,Marius Wernig,Jacob H. Hanna,Andrey Sivachenko,Xiaolan Zhang,Bradley E. Bernstein,Bradley E. Bernstein,Chad Nusbaum,David B. Jaffe,Andreas Gnirke,Rudolf Jaenisch,Eric S. Lander +14 more
TL;DR: Low-throughput reduced representation bisulphite sequencing is established as a powerful technology for epigenetic profiling of cell populations relevant to developmental biology, cancer and regenerative medicine.