Simple Combinations of Lineage-Determining Transcription Factors Prime cis-Regulatory Elements Required for Macrophage and B Cell Identities
Sven Heinz,Christopher Benner,Nathanael J. Spann,Eric Bertolino,Yin C. Lin,Peter Laslo,Jason X. Cheng,Cornelis Murre,Harinder Singh,Harinder Singh,Christopher K. Glass +10 more
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TLDR
It is demonstrated in macrophages and B cells that collaborative interactions of the common factor PU.1 with small sets of macrophage- or B cell lineage-determining transcription factors establish cell-specific binding sites that are associated with the majority of promoter-distal H3K4me1-marked genomic regions.About:
This article is published in Molecular Cell.The article was published on 2010-05-28 and is currently open access. It has received 9620 citations till now. The article focuses on the topics: Pioneer factor & General transcription factor.read more
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Therapeutic targeting of ependymoma as informed by oncogenic enhancer profiling
Stephen C. Mack,Kristian W. Pajtler,Kristian W. Pajtler,Lukas Chavez,Lukas Chavez,Konstantin Okonechnikov,Kelsey C. Bertrand,Kelsey C. Bertrand,Xiuxing Wang,Xiuxing Wang,Xiuxing Wang,Serap Erkek,Alexander J. Federation,Anne Song,Anne Song,Christine Lee,Christine Lee,Xin Wang,Laura McDonald,James J. Morrow,Alina Saiakhova,Patrick Sin-Chan,Qiulian Wu,Qiulian Wu,Qiulian Wu,Kulandaimanuvel Antony Michaelraj,Tyler E. Miller,Tyler E. Miller,Christopher G. Hubert,Christopher G. Hubert,Marina Ryzhova,Livia Garzia,Laura K. Donovan,Stephen M. Dombrowski,Stephen M. Dombrowski,Daniel C. Factor,Betty Luu,Claudia L.L. Valentim,Claudia L.L. Valentim,Ryan C. Gimple,Ryan C. Gimple,Ryan C. Gimple,Andrew R. Morton,Andrew R. Morton,Leo J.Y. Kim,Leo J.Y. Kim,Leo J.Y. Kim,Briana C. Prager,Briana C. Prager,Briana C. Prager,John J.Y. Lee,Xiaochong Wu,Jennifer Zuccaro,Yuan Yao Thompson,Borja L. Holgado,Jüri Reimand,Jüri Reimand,Susan Q. Ke,Susan Q. Ke,Adam Tropper,Adam Tropper,Sisi Lai,Sisi Lai,Senthuran Vijayarajah,Senthuran Vijayarajah,Sylvia Doan,Vaidehi Mahadev,Vaidehi Mahadev,Ana Fernandez Miñan,Susanne Gröbner,Matthias Lienhard,Marc Zapatka,Zhiqin Huang,Kenneth Aldape,Angel M. Carcaboso,Peter J. Houghton,Stephen T. Keir,Till Milde,Till Milde,Hendrik Witt,Hendrik Witt,Yan Li,Chao Jun Li,Xiu-Wu Bian,David T.W. Jones,Ian C. Scott,Sheila K. Singh,Annie Huang,Annie Huang,Peter B. Dirks,Eric Bouffet,Eric Bouffet,James E. Bradner,Vijay Ramaswamy,Nada Jabado,James T. Rutka,Paul A. Northcott,Mathieu Lupien,Peter Lichter,Andrey Korshunov,Andrey Korshunov,Peter C. Scacheri,Stefan M. Pfister,Stefan M. Pfister,Marcel Kool,Michael D. Taylor,Jeremy N. Rich,Jeremy N. Rich,Jeremy N. Rich +108 more
TL;DR: Through profiling of transcriptional enhancers, this study provides a framework for target and drug discovery in other cancers that lack known genetic drivers and are therefore difficult to treat, and reveals putative oncogenes, molecular targets and pathways.
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DNA N6-Methyladenine Is Dynamically Regulated in the Mouse Brain Following Environmental Stress
Bing Yao,Ying Cheng,Zhiqin Wang,Yujing Li,Li Chen,Li Chen,Luoxiu Huang,Weiya Zhang,Dahua Chen,Hao Wu,Beisha Tang,Peng Jin +11 more
TL;DR: It is shown that N6-methyladenine level in the mouse brain is dynamic following environmental stress, and the subsequent differential gene expression is correlated with LINE transposon expression, suggesting an epigenetic role for 6mA in the mammalian brain as well as its potential involvement in neuropsychiatric disorders.
Journal ArticleDOI
PRC1 Catalytic Activity Is Central to Polycomb System Function
Neil P. Blackledge,Nadezda A. Fursova,Jessica R. Kelley,Miles K. Huseyin,Angelika Feldmann,Robert J. Klose +5 more
TL;DR: It is found that Polycomb-mediated gene repression requires PRC1 catalytic activity, and this discovery provides compelling evidence thatPRC1 catalysis is central to Polycomb system function and gene regulation.
Journal ArticleDOI
Distinct epigenetic programs regulate cardiac myocyte development and disease in the human heart in vivo
Ralf Gilsbach,Martin Schwaderer,Sebastian Preissl,Björn Grüning,David Kranzhoefer,Pedro Schneider,Thomas G Nuehrenberg,Sonia Mulero-Navarro,Dieter Weichenhan,Christian Braun,M. Dreßen,Adam Jacobs,Harald Lahm,Torsten Doenst,Rolf Backofen,Markus Krane,Bruce D. Gelb,Lutz Hein +17 more
TL;DR: This study uncovers distinct layers of epigenetic regulation not only during prenatal development and postnatal maturation but also in diseased human cardiac myocytes.
Journal ArticleDOI
Emergence of a High-Plasticity Cell State during Lung Cancer Evolution
Nemanja D. Marjanovic,Nemanja D. Marjanovic,Matan Hofree,Jason E. Chan,David Canner,Katherine Wu,Marianna Trakala,Griffin Hartmann,Olivia Smith,Olivia Smith,Jonathan Y. Kim,Jonathan Y. Kim,Kelly V. Evans,Anna Hudson,Orr Ashenberg,Caroline B. M. Porter,Alborz Bejnood,Ayshwarya Subramanian,Kenneth L. Pitter,Yan Yan,Toni Delorey,Devan Phillips,Nisargbhai S. Shah,Ojasvi Chaudhary,Alexander M. Tsankov,Alexander M. Tsankov,Travis J. Hollmann,Natasha Rekhtman,Pierre P. Massion,John T. Poirier,Linas Mazutis,Ruifang Li,Joo-Hyeon Lee,Angelika Amon,Charles M. Rudin,Tyler Jacks,Aviv Regev,Tuomas Tammela,Tuomas Tammela +38 more
TL;DR: This study reveals a central principle underpinning intra-tumoral heterogeneity and motivates therapeutic targeting of the HPCS, which is associated with poor survival across human cancers and demonstrates chemoresistance in mice.
References
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RAG-1-deficient mice have no mature B and T lymphocytes
Peter Mombaerts,John Iacomini,Randall S. Johnson,Karl Herrup,Susumu Tonegawa,Virginia E. Papaioannou +5 more
TL;DR: The introduction of a mutation in RAG-1 into the germline of mice via gene targeting in embryonic stem cells is described and it is shown that this mutation either activates or catalyzes the V(D)J recombination reaction of immunoglobulin and T cell receptor genes.
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Integration of External Signaling Pathways with the Core Transcriptional Network in Embryonic Stem Cells
Xi Chen,Han Xu,Ping Yuan,Fang Fang,Fang Fang,Mikael Huss,Vinsensius B. Vega,Eleanor Wong,Yuriy L. Orlov,Weiwei Zhang,Weiwei Zhang,Jianming Jiang,Jianming Jiang,Yuin-Han Loh,Yuin-Han Loh,Hock Chuan Yeo,Zhen Xuan Yeo,Vipin Narang,Kunde R Govindarajan,Bernard Leong,Atif Shahab,Yijun Ruan,Guillaume Bourque,Wing-Kin Sung,Neil D. Clarke,Chia-Lin Wei,Huck-Hui Ng,Huck-Hui Ng +27 more
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Genome-scale DNA methylation maps of pluripotent and differentiated cells
Alexander Meissner,Tarjei S. Mikkelsen,Tarjei S. Mikkelsen,Hongcang Gu,Marius Wernig,Jacob H. Hanna,Andrey Sivachenko,Xiaolan Zhang,Bradley E. Bernstein,Bradley E. Bernstein,Chad Nusbaum,David B. Jaffe,Andreas Gnirke,Rudolf Jaenisch,Eric S. Lander +14 more
TL;DR: Low-throughput reduced representation bisulphite sequencing is established as a powerful technology for epigenetic profiling of cell populations relevant to developmental biology, cancer and regenerative medicine.