Simple Combinations of Lineage-Determining Transcription Factors Prime cis-Regulatory Elements Required for Macrophage and B Cell Identities
Sven Heinz,Christopher Benner,Nathanael J. Spann,Eric Bertolino,Yin C. Lin,Peter Laslo,Jason X. Cheng,Cornelis Murre,Harinder Singh,Harinder Singh,Christopher K. Glass +10 more
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TLDR
It is demonstrated in macrophages and B cells that collaborative interactions of the common factor PU.1 with small sets of macrophage- or B cell lineage-determining transcription factors establish cell-specific binding sites that are associated with the majority of promoter-distal H3K4me1-marked genomic regions.About:
This article is published in Molecular Cell.The article was published on 2010-05-28 and is currently open access. It has received 9620 citations till now. The article focuses on the topics: Pioneer factor & General transcription factor.read more
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A cell identity switch allows residual BCC to survive Hedgehog pathway inhibition.
Brian Biehs,Gerrit J. P. Dijkgraaf,Robert Piskol,Bruno Alicke,Soufiane Boumahdi,Franklin Peale,Stephen E. Gould,Frederic J. de Sauvage +7 more
TL;DR: Treatment of BCC with both vismodegib and a Wnt pathway inhibitor reduced the residual tumour burden and enhanced differentiation and identified a resistance mechanism in which tumour cells evade treatment by adopting an alternative identity that does not rely on the original oncogenic driver for survival.
Journal ArticleDOI
The Nuclear Receptor PPARγ Controls Progressive Macrophage Polarization as a Ligand-Insensitive Epigenomic Ratchet of Transcriptional Memory.
Bence Daniel,Gergely Nagy,Zsolt Czimmerer,Attila Horvath,David W. Hammers,Ixchelt Cuaranta-Monroy,Szilárd Póliska,Petros Tzerpos,Zsuzsanna Kolostyak,Tristan T. Hays,Andreas Patsalos,René Houtman,Sascha Sauer,Sascha Sauer,Sascha Sauer,Jean Francois-Deleuze,Fraydoon Rastinejad,Balint L. Balint,H. Lee Sweeney,Laszlo Nagy,Laszlo Nagy +20 more
TL;DR: It is found that the nuclear receptor PPAR&ggr; drove robust phenotypic changes in macrophages upon repeated stimulation with interleukin (IL)‐4, and has a significant ligand‐insensitive, genome‐bound fraction that affects local chromatin structure upon macrophage polarization.
Journal ArticleDOI
Condensin I and II Complexes License Full Estrogen Receptor α-Dependent Enhancer Activation
Wenbo Li,Yiren Hu,Soohwan Oh,Qi Ma,Daria Merkurjev,Xiaoyuan Song,Xiang Zhou,Zhijie Liu,Bogdan Tanasa,Xin He,Aaron Yun Chen,Kenny Ohgi,Jie Zhang,Wen Liu,Michael G. Rosenfeld +14 more
TL;DR: Both condensin complexes exhibit an unexpected, dramatic estrogen-induced recruitment to estrogen receptor α (ER-α)-bound eRNA(+) active enhancers in interphase breast cancer cells, exhibiting non-canonical interaction with ER-α via its DNA-binding domain (DBD).
Journal ArticleDOI
Small-molecule targeting of brachyury transcription factor addiction in chordoma.
Tanaz Sharifnia,Mathias Wawer,Ting Chen,Qingyuan Huang,Qingyuan Huang,Barbara A. Weir,Barbara A. Weir,Ann E. Sizemore,Ann E. Sizemore,Matthew A. Lawlor,Amy Goodale,Glenn S. Cowley,Glenn S. Cowley,Francisca Vazquez,Christopher J. Ott,Joshua M. Francis,Slim Sassi,Patricia Cogswell,Hadley E. Sheppard,Tinghu Zhang,Nathanael S. Gray,Paul A. Clarke,Julian Blagg,Paul Workman,Josh Sommer,Francis J. Hornicek,Francis J. Hornicek,David E. Root,William C. Hahn,William C. Hahn,James E. Bradner,James E. Bradner,Kwok K. Wong,Paul A. Clemons,Charles Y. Lin,Joanne Kotz,Stuart L. Schreiber,Stuart L. Schreiber,Stuart L. Schreiber +38 more
TL;DR: Together, these data demonstrate small-molecule targeting of brachyury transcription factor addiction in chordoma, identify a mechanism of T gene regulation that underlies this therapeutic strategy, and provide a blueprint for applying systematic genetic and chemical screening approaches to discover vulnerabilities in genomically quiet cancers.
Journal ArticleDOI
Hepatocyte Nuclear Factors 4α and 1α Regulate Kidney Developmental Expression of Drug-Metabolizing Enzymes and Drug Transporters
TL;DR: The data strongly supports a critical role for HNF4a and Hnf1a in the tissue-specific regulation of drug handling and differentiation toward a PT-like cellular identity.
References
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RAG-1-deficient mice have no mature B and T lymphocytes
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TL;DR: The introduction of a mutation in RAG-1 into the germline of mice via gene targeting in embryonic stem cells is described and it is shown that this mutation either activates or catalyzes the V(D)J recombination reaction of immunoglobulin and T cell receptor genes.
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Integration of External Signaling Pathways with the Core Transcriptional Network in Embryonic Stem Cells
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TL;DR: This study uses chromatin immunoprecipitation coupled with ultra-high-throughput DNA sequencing to map the locations of TF-binding sites and identifies important features of the transcriptional regulatory networks that define ES-cell identity.
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Genome-scale DNA methylation maps of pluripotent and differentiated cells
Alexander Meissner,Tarjei S. Mikkelsen,Tarjei S. Mikkelsen,Hongcang Gu,Marius Wernig,Jacob H. Hanna,Andrey Sivachenko,Xiaolan Zhang,Bradley E. Bernstein,Bradley E. Bernstein,Chad Nusbaum,David B. Jaffe,Andreas Gnirke,Rudolf Jaenisch,Eric S. Lander +14 more
TL;DR: Low-throughput reduced representation bisulphite sequencing is established as a powerful technology for epigenetic profiling of cell populations relevant to developmental biology, cancer and regenerative medicine.