Genetic compensation: A phenomenon in search of mechanisms.
TLDR
This review revisits studies reporting genetic compensation in higher eukaryotes and outlines possible molecular mechanisms, which may include both transcriptional and posttranscriptional processes.Abstract:
Several recent studies in a number of model systems including zebrafish, Arabidopsis, and mouse have revealed phenotypic differences between knockouts (i.e., mutants) and knockdowns (e.g., antisense-treated animals). These differences have been attributed to a number of reasons including off-target effects of the antisense reagents. An alternative explanation was recently proposed based on a zebrafish study reporting that genetic compensation was observed in egfl7 mutant but not knockdown animals. Dosage compensation was first reported in Drosophila in 1932, and genetic compensation in response to a gene knockout was first reported in yeast in 1969. Since then, genetic compensation has been documented many times in a number of model organisms; however, our understanding of the underlying molecular mechanisms remains limited. In this review, we revisit studies reporting genetic compensation in higher eukaryotes and outline possible molecular mechanisms, which may include both transcriptional and posttranscriptional processes.read more
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Activation of Rho Family GTPases by Small Molecules.
Charuta C. Palsuledesai,Zurab Surviladze,Anna Waller,T Fabiola Miscioscia,Yuna Guo,Yang Wu,Jake Strouse,Elsa Romero,Virginia M. Salas,Ramona Curpan,Susan Young,Mark B. Carter,Terry D. Foutz,Zhanna Galochkina,Harold A. Ames,Mark K. Haynes,Bruce S. Edwards,Orazio Nicolotti,Li Luo,Oleg Ursu,Cristian Bologa,Tudor I. Oprea,Angela Wandinger-Ness,Larry A. Sklar +23 more
TL;DR: The characterization of small molecule agonists of one or more of six Rho, Rab, and Ras family GTPases that were first identified through flow cytometry-based, multiplexed high-throughput screening of 200000 compounds confirmed agonist activity of 43 compounds.
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Building the vertebrate codex using the gene breaking protein trap library
Noriko Ichino,MaKayla R Serres,Rhianna M. Urban,Mark D. Urban,Anthony J. Treichel,Kyle J. Schaefbauer,Lauren E Greif,Gaurav K. Varshney,Gaurav K. Varshney,Kimberly J. Skuster,Melissa S. McNulty,Camden L. Daby,Ying Wang,Hsin-kai Liao,Suzan El-Rass,Yonghe Ding,Weibin Liu,Jennifer L. Anderson,Mark D Wishman,Ankit Sabharwal,Lisa A. Schimmenti,Sridhar Sivasubbu,Darius Balciunas,Matthias Hammerschmidt,Steven A. Farber,Xiao-Yan Wen,Xiaolei Xu,Maura McGrail,Jeffrey J. Essner,Shawn M. Burgess,Karl J. Clark,Stephen C. Ekker +31 more
TL;DR: A collection of 1200 transgenic zebrafish strains made with the gene-break transposon (GBT) protein trap is reported to simultaneously report and reversibly knockdown the tagged genes.
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Oxytocin receptor is not required for social attachment in prairie voles
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The analysis of living systems can generate both knowledge and illusions
TL;DR: A broad classification of techniques into six complementary approaches is proposed: perturbation, visualization, substitution, characterization, reconstitution, and simulation to help increase the reproducibility of inferences and improve peer review.
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Divergent Hemogen genes of teleosts and mammals share conserved roles in erythropoiesis: analysis using transgenic and mutant zebrafish.
Michael Peters,Sandra K. Parker,Jeffrey M. Grim,Corey A. H. Allard,Jonah Levin,H. William Detrich +5 more
TL;DR: Transgenic and mutant zebrafish lines were created to characterize the expression and functions of Hemogen, a transcription factor involved in the formation of red blood cells and other processes, and it is demonstrated that Hemogens are largely composed of 21-25 amino acid repeats.
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