Structural and Functional Analysis of the D614G SARS-CoV-2 Spike Protein Variant.
Leonid Yurkovetskiy,Xue Wang,Kristen E. Pascal,Christopher Tomkins-Tinch,Thomas Nyalile,Yetao Wang,Alina Baum,William E. Diehl,Ann Dauphin,Claudia Carbone,Kristen Veinotte,Shawn B. Egri,Stephen F. Schaffner,Stephen F. Schaffner,Jacob E. Lemieux,James B. Munro,Ashique Rafique,Abhi Barve,Pardis C. Sabeti,Christos A. Kyratsous,Natalya Dudkina,Kuang Shen,Jeremy Luban +22 more
TLDR
It is shown that D614G was more infectious than the ancestral form on human lung cells, colon cells, and on cells rendered permissive by ectopic expression of human ACE2 or of ACE2 orthologs from various mammals, including Chinese rufous horseshoe bat and Malayan pangolin.About:
This article is published in Cell.The article was published on 2020-10-29 and is currently open access. It has received 840 citations till now. The article focuses on the topics: Virion membrane & Protein structure.read more
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D614G substitution at the hinge region enhances the stability of trimeric SARS-CoV-2 spike protein
TL;DR: Results suggest that D614G substitution could modulate the flexibility of spike protein and confer enhanced thermodynamic stability and hint that G614 likely increases the availability of functional form of spike trimer thereby associated to increased infectivity.
Journal ArticleDOI
Detecting Potentially Adaptive Mutations from the Parallel and Fixed Patterns in SARS-CoV-2 Evolution
Chengyang Ji,Na Han,Yegang Cheng,Jingzhe Shang,Shenghui Weng,Rong Yang,Hangyu Zhou,Aiping Wu +7 more
TL;DR: It is found that the biological meanings of fixed substitution and parallel mutation are highly complementary, and can reasonably be integrated as a fixed and parallel (paraFix) mutation, to identify potentially adaptive mutations.
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The fatty acid site is coupled to functional motifs in the SARS-CoV-2 spike protein and modulates spike allosteric behaviour
Ana Sofia F Oliveira,Deborah K. Shoemark,Amaurys Avila Ibarra,Andrew D. Davidson,Imre Berger,Christiane Schaffitzel,Adrian J. Mulholland +6 more
TL;DR: Toelzer et al. as mentioned in this paper showed that removal of a ligand from the fatty acid binding site significantly affects the dynamics of distant, functionally important regions of the spike, including the receptor-binding motif, furin cleavage site and fusion-peptide-adjacent regions.
Journal ArticleDOI
COVID-19 outbreak in Malaysia: Decoding D614G mutation of SARS-CoV-2 virus isolated from an asymptomatic case in Pahang
TL;DR: In this paper , the authors investigated the genome identity of circulating COVID-19 strains in Pahang and understand disease epidemiology during the pandemic by leveraging high-throughput sequencing analysis for the whole genome sequencing and implemented bioinformatic technique for the analysis.
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Mutational hotspot in the SARS-CoV-2 Spike protein N-terminal domain conferring immune escape potential
TL;DR: In this article, the authors investigate the impact of mutations on the evasion of neutralizing antibodies and uncover that NTD is a region exhibiting a particularly high frequency of mutation recruitments, suggesting that the virus maintains optimal efficiency of ACE2 binding combined with the flexibility facilitating the immune escape.
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