Structural and Functional Analysis of the D614G SARS-CoV-2 Spike Protein Variant.
Leonid Yurkovetskiy,Xue Wang,Kristen E. Pascal,Christopher Tomkins-Tinch,Thomas Nyalile,Yetao Wang,Alina Baum,William E. Diehl,Ann Dauphin,Claudia Carbone,Kristen Veinotte,Shawn B. Egri,Stephen F. Schaffner,Stephen F. Schaffner,Jacob E. Lemieux,James B. Munro,Ashique Rafique,Abhi Barve,Pardis C. Sabeti,Christos A. Kyratsous,Natalya Dudkina,Kuang Shen,Jeremy Luban +22 more
TLDR
It is shown that D614G was more infectious than the ancestral form on human lung cells, colon cells, and on cells rendered permissive by ectopic expression of human ACE2 or of ACE2 orthologs from various mammals, including Chinese rufous horseshoe bat and Malayan pangolin.About:
This article is published in Cell.The article was published on 2020-10-29 and is currently open access. It has received 840 citations till now. The article focuses on the topics: Virion membrane & Protein structure.read more
Citations
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The Mechanism and Consequences of SARS-CoV-2 Spike-Mediated Fusion and Syncytia Formation
TL;DR: In this paper , the authors discuss the molecular determinants of S mediated fusion and the antiviral innate immunity components that counteract syncytia formation in SARS-CoV-2 infection and pathology.
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The SARS-CoV-2 and other human coronavirus spike proteins are fine-tuned towards temperature and proteases of the human airways
Manon Laporte,Annelies Stevaert,Valerie Raeymaekers,Ria Van Berwaer,Katleen Martens,Stefan Pöhlmann,Lieve Naesens +6 more
TL;DR: This work reveals features of the coronavirus spike (S) protein, which optimize the virus towards different parts of the respiratory tract, and identified airway proteases that activate the spike for virus entry into cells, including one protease that may mediate coronav virus virulence.
Journal ArticleDOI
Structural basis for the different states of the spike protein of SARS-CoV-2 in complex with ACE2.
Journal ArticleDOI
Infection and transmission of ancestral SARS-CoV-2 and its alpha variant in pregnant white-tailed deer.
Konner Cool,Natasha N. Gaudreault,Igor Morozov,Jessie D. Trujillo,David A. Meekins,Chester McDowell,Mariano Carossino,Dashzeveg Bold,Dana N Mitzel,Taeyong Kwon,Velmurugan Balaraman,Daniel W. Madden,Bianca Libanori Artiaga,Roman Pogranichniy,Gleyder Roman Sosa,Jamie Henningson,William C. Wilson,Udeni B. R. Balasuriya,Adolfo García-Sastre,Juergen A. Richt +19 more
TL;DR: In this paper, the authors investigated the susceptibility and transmission of SARS-CoV-2 in adult white-tailed deer (WTD) and found that adult WTD are highly susceptible to SARS infection and can transmit the virus through direct contact as well as vertically from doe to fetus.
Journal ArticleDOI
Characterization of the SARS-CoV-2 S Protein: Biophysical, Biochemical, Structural, and Antigenic Analysis
Natalia G. Herrera,Nicholas C. Morano,A. Celikgil,George I. Georgiev,Ryan J. Malonis,James H. Lee,Karen Tong,Olivia Vergnolle,Aldo Massimi,Laura Y. Yen,Alex J. Noble,Mykhailo Kopylov,Jeffrey B. Bonanno,Sarah C. Garrett-Thomson,David B. Hayes,Robert H. Bortz,Ariel S. Wirchnianski,Catalina Florez,Catalina Florez,Ethan Laudermilch,Denise Haslwanter,J. Maximilian Fels,M. Eugenia Dieterle,Rohit K. Jangra,Jason Barnhill,Amanda Mengotto,Duncan Kimmel,Johanna P. Daily,Liise Anne Pirofski,Kartik Chandran,Michael Brenowitz,Scott J. Garforth,Edward T. Eng,Jonathan R. Lai,S.C. Almo +34 more
TL;DR: In this article, the authors evaluated the expression and purification of two previously reported S protein constructs in Expi293F and ExpiCHO-S cells, two different cell lines selected for increased protein expression.
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