Structural and Functional Analysis of the D614G SARS-CoV-2 Spike Protein Variant.
Leonid Yurkovetskiy,Xue Wang,Kristen E. Pascal,Christopher Tomkins-Tinch,Thomas Nyalile,Yetao Wang,Alina Baum,William E. Diehl,Ann Dauphin,Claudia Carbone,Kristen Veinotte,Shawn B. Egri,Stephen F. Schaffner,Stephen F. Schaffner,Jacob E. Lemieux,James B. Munro,Ashique Rafique,Abhi Barve,Pardis C. Sabeti,Christos A. Kyratsous,Natalya Dudkina,Kuang Shen,Jeremy Luban +22 more
TLDR
It is shown that D614G was more infectious than the ancestral form on human lung cells, colon cells, and on cells rendered permissive by ectopic expression of human ACE2 or of ACE2 orthologs from various mammals, including Chinese rufous horseshoe bat and Malayan pangolin.About:
This article is published in Cell.The article was published on 2020-10-29 and is currently open access. It has received 840 citations till now. The article focuses on the topics: Virion membrane & Protein structure.read more
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Impact of SARS-CoV-2 B.1.1.7 Spike variant on neutralisation potency of sera from individuals vaccinated with Pfizer vaccine BNT162b2
Dami A. Collier,Anna De Marco,Isabella Ferreira,Bo Meng,Rawlings Datir,Rawlings Datir,Alexandra C. Walls,Steven A. Kemp S,Steven A. Kemp S,Jessica Bassi,Dora Pinto,Chiara Silacci Fregni,Siro Bianchi,M. Alejandra Tortorici,John E. Bowen,Katja Culap,Stefano Jaconi,Elisabetta Cameroni,Gyorgy Snell,Matteo Samuele Pizzuto,Alessandra Franzetti Pellanda,Christian Garzoni,Agostino Riva,Anne Elmer,Nathalie Kingston,Barbara J. Graves,Laura E. McCoy,Kenneth G. C. Smith,John Bradley,Ceron Ceron-Gutierrez L,Gabriela Barcenas-Morales,Herbert W. Virgin,Antonio Lanzavecchia,Luca Piccoli,Rainer Doffinger,Mark R. Wills,David Veesler,Davide Corti,Ravindra K. Gupta +38 more
TL;DR: In this paper, the authors measured neutralizing antibody responses following a single immunization using pseudovirus expressing the wild-type Spike protein or the 8 amino acid mutations found in the B.1.7 spike protein.
Posted ContentDOI
Neutralising antibodies in Spike mediated SARS-CoV-2 adaptation.
Steven Kemp,Dami A. Collier,Dami A. Collier,Rawlings Datir,Rawlings Datir,Iatm Ferreira,S Gayed,Aminu S Jahun,Myra Hosmillo,Chloe Rees-Spear,Petra Mlcochova,Ines Ushiro Lumb,David J. Roberts,Anita Chandra,Nigel Temperton,Katherine Sharrocks,E Blane,Jag Briggs,MJ van Gils,Kgc Smith,John Bradley,Chris Smith,Rainer Doffinger,Lourdes Ceron-Gutierrez,G. Barcenas-Morales,David D. Pollock,Richard A. Goldstein,Anna Smielewska,Jordan P. Skittrall,Theodore Gouliouris,Ian Goodfellow,Effrossyni Gkrania-Klotsas,Cjr Illingworth,Laura E. McCoy,Ravindra K. Gupta +34 more
TL;DR: These data reveal strong positive selection on SARS-CoV-2 during convalescent plasma therapy and identify the combination of Spike mutations D796H and deltaH69/deltaV70 as a broad antibody resistance mechanism against commonly occurring antibody responses to Sars-Cov-2.
Journal ArticleDOI
The next phase of SARS-CoV-2 surveillance: real-time molecular epidemiology.
Bas B. Oude Munnink,Nathalie Worp,David F. Nieuwenhuijse,Reina S. Sikkema,Bart L. Haagmans,Ron A. M. Fouchier,Marion Koopmans +6 more
TL;DR: The current coronavirus disease 2019 (COVID-19) pandemic is the first to apply whole-genome sequencing near to real time, with over 2 million severe acute respiratory syndrome (SARS-CoV-2) wholegenome sequences generated and shared through the GISAID platform as discussed by the authors.
Journal ArticleDOI
SARS-COV-2 Variants: Differences and Potential of Immune Evasion
Sandro M. Hirabara,Tamires Duarte Afonso Serdan,Renata Gorjão,Laureane Nunes Masi,Tania Cristina Pithon-Curi,Dimas Tadeu Covas,Rui Curi,Edison Luiz Durigon +7 more
TL;DR: This review addressed the following critical points concerning VOCs: characteristics of the SARS-CoV-2 V OCs with mutations in the S gene, possible evasion of variants from neutralizing antibodies generated through vaccination, previous infection, or immune therapies, potential risk of new pandemic waves induced by the variants worldwide, and perspectives for further studies and actions.
Journal ArticleDOI
The SARS-CoV-2 Spike variant D614G favors an open conformational state.
Rachael A. Mansbach,Srirupa Chakraborty,Kien Nguyen,David C. Montefiori,Bette T. Korber,Sandrasegaram Gnanakaran +5 more
TL;DR: In this article, the authors show that changes in the protein energetics favor a higher population of infection-capable states in the G-form through release of asymmetry present in the D-form inter-protomer interactions.
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