Structural and Functional Analysis of the D614G SARS-CoV-2 Spike Protein Variant.
Leonid Yurkovetskiy,Xue Wang,Kristen E. Pascal,Christopher Tomkins-Tinch,Thomas Nyalile,Yetao Wang,Alina Baum,William E. Diehl,Ann Dauphin,Claudia Carbone,Kristen Veinotte,Shawn B. Egri,Stephen F. Schaffner,Stephen F. Schaffner,Jacob E. Lemieux,James B. Munro,Ashique Rafique,Abhi Barve,Pardis C. Sabeti,Christos A. Kyratsous,Natalya Dudkina,Kuang Shen,Jeremy Luban +22 more
TLDR
It is shown that D614G was more infectious than the ancestral form on human lung cells, colon cells, and on cells rendered permissive by ectopic expression of human ACE2 or of ACE2 orthologs from various mammals, including Chinese rufous horseshoe bat and Malayan pangolin.About:
This article is published in Cell.The article was published on 2020-10-29 and is currently open access. It has received 840 citations till now. The article focuses on the topics: Virion membrane & Protein structure.read more
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SARS-CoV-2 Subgenomic RNAs: Characterization, Utility, and Perspectives.
TL;DR: In this article, a review summarizes the recent progress on SARS-CoV-2 sgRNA identification, characterization, and application as a viral replication marker, and potential future research areas of interest are discussed.
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Reprogrammed CRISPR-Cas13b suppresses SARS-CoV-2 replication and circumvents its mutational escape through mismatch tolerance.
Mohamed Fareh,Mohamed Fareh,Wei Zhao,Wenxin Hu,Wenxin Hu,Joshua M. L. Casan,Joshua M. L. Casan,Amit Kumar,Amit Kumar,Jori Symons,Jennifer M. Zerbato,Danielle Fong,Ilia Voskoboinik,Ilia Voskoboinik,Paul G Ekert,Rajeev Rudraraju,Rajeev Rudraraju,Damian F. J. Purcell,Sharon R Lewin,Sharon R Lewin,Sharon R Lewin,Joseph A. Trapani,Joseph A. Trapani +22 more
TL;DR: In this paper, the authors employ genome-wide computational prediction and single-nucleotide resolution screening to reprogram CRISPR-Cas13b against SARS-CoV-2 genomic and subgenomic RNAs.
Journal ArticleDOI
Whole-genome sequencing of SARS-CoV-2 reveals the detection of G614 variant in Pakistan.
Massab Umair,Aamer Ikram,Muhammad Salman,Adnan Khurshid,Masroor Alam,Nazish Badar,Rana Muhammad Suleman,Faheem Tahir,Salmaan Sharif,Joel M. Montgomery,Shannon L M Whitmer,John D. Klena +11 more
TL;DR: In this paper, the authors reported the sequencing, genomic characterization, and phylogenetic analysis of five SARS-CoV-2 strains isolated from patients in Pakistan and used NEBNext Ultra II Directional RNA Library Prep kit for Illumina (NEW ENGLAND BioLabs Inc., MA, US) and Illumina iSeq 100 instrument (Illumina, San Diego, US).
Posted ContentDOI
Impact of the B.1.1.7 variant on neutralizing monoclonal antibodies recognizing diverse epitopes on SARS-CoV-2 Spike
Luke Muir,Weng M Ng,Helen M. E. Duyvesteyn,Yuguang Zhao,Thomas A. Bowden,Annachiara Rosa,Peter Cherepanov,Laura E. McCoy +7 more
TL;DR: In this article, neutralizing antibodies targeting subdominant epitopes of the SARS-CoV-2 Spike receptor binding domain (RBD) have been found to have a glycan-dependant neutralization activity.
Journal ArticleDOI
Inter-domain communication in SARS-CoV-2 spike proteins controls protease-triggered cell entry
Enya Qing,Pengfei Li,Laura M. Cooper,Sebastian R. Schulz,Hans-Martin Jäck,Lijun Rong,Stanley Perlman,Tom Gallagher +7 more
TL;DR: In this paper , the authors identify an inter-monomer β sheet structure that facilitates NTD-to-FD transmissions and subsequent fusion activation, indicating allosteric inter-domain control of fusion activation.
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