Structural and Functional Analysis of the D614G SARS-CoV-2 Spike Protein Variant.
Leonid Yurkovetskiy,Xue Wang,Kristen E. Pascal,Christopher Tomkins-Tinch,Thomas Nyalile,Yetao Wang,Alina Baum,William E. Diehl,Ann Dauphin,Claudia Carbone,Kristen Veinotte,Shawn B. Egri,Stephen F. Schaffner,Stephen F. Schaffner,Jacob E. Lemieux,James B. Munro,Ashique Rafique,Abhi Barve,Pardis C. Sabeti,Christos A. Kyratsous,Natalya Dudkina,Kuang Shen,Jeremy Luban +22 more
TLDR
It is shown that D614G was more infectious than the ancestral form on human lung cells, colon cells, and on cells rendered permissive by ectopic expression of human ACE2 or of ACE2 orthologs from various mammals, including Chinese rufous horseshoe bat and Malayan pangolin.About:
This article is published in Cell.The article was published on 2020-10-29 and is currently open access. It has received 840 citations till now. The article focuses on the topics: Virion membrane & Protein structure.read more
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Mechanical activation of spike fosters SARS-CoV-2 viral infection.
Wei Hu,Yong Zhang,Panyu Fei,Tongtong Zhang,Danmei Yao,Yufei Gao,Jia Liu,Hui Chen,Qiao Lu,Tenny Mudianto,Xinrui Zhang,Chuxuan Xiao,Yang Ye,Qiming Sun,Jing Zhang,Qi Xie,Pei-Hui Wang,Jun Wang,Zhenhai Li,Jizhong Lou,Wei Chen +20 more
TL;DR: In this paper, tensile force, generated by bending of the host cell membrane, strengthened spike recognition of ACE2 and accelerated the detachment of spike's S1 subunit from the S2 subunit to rapidly prime the viral fusion machinery.
Journal ArticleDOI
Protection against reinfection with D614- or G614-SARS-CoV-2 isolates in golden Syrian hamster.
Marco Brustolin,Jordi Rodon,María Luisa Rodríguez de la Concepción,Carlos Ávila-Nieto,Guillermo Cantero,Mónica Pérez,Nigeer Te,Marc Noguera-Julian,Victor Guallar,Victor Guallar,Alfonso Valencia,Alfonso Valencia,Nuria Roca,Nuria Izquierdo-Useros,Julià Blanco,Bonaventura Clotet,Albert Bensaid,Jorge Carrillo,Júlia Vergara-Alert,Joaquim Segalés +19 more
TL;DR: The real incidence of SARS-CoV-2 infection in humans is unknown as mentioned in this paper, although its real incidence is currently unknown, and this phenomenon has a great impact on public health, this phenomenon raises the que...
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SARS-CoV-2 B.1.1.7 escape from mRNA vaccine-elicited neutralizing antibodies
Ravindra K. Gupta,Dami A. Collier,Dami A. Collier,Anna De Marco,Isabella Ferreira,Bo Meng,Rawlings Datir,Rawlings Datir,Alexandra C. Walls,Steven Kemp,Jessica Bassi,Dora Pinto,Chiara Silacci-Fregni,Siro Bianchi,M. Alejandra Tortorici,John E. Bowen,Katja Culap,Stefano Jaconi,Elisabetta Cameroni,Gyorgy Snell,Matteo Samuele Pizzuto,Allessandra Pallanda,Christian Gorzoni,Agostino Riva,Anne Elmer,Barbara J. Graves,Laura E. McCoy,Kenneth G. C. Smith,John Bradley,James Thaventhiran,Lourdes Ceron-Gutierrez,Gabriela Barcenas-Morales,Herbert W. Virgin,Antonio Lanzavecchia,Luca Piccoli,Rainer Doffinger,Mark R. Wills,David Veesler,Davide Corti +38 more
TL;DR: Assessment of immune responses following vaccination with mRNA-based vaccine BNT162b2 measured neutralising antibody responses following a single immunization using pseudoviruses expressing the wild-type Spike protein or the 8 mutations found in the B.1.1-CoV-2 Spike protein.
Journal ArticleDOI
D614G Substitution of SARS-CoV-2 Spike Protein Increases Syncytium Formation and Virus Titer via Enhanced Furin-Mediated Spike Cleavage.
Ya Wen Cheng,Tai Ling Chao,Chiao Ling Li,Sheng Han Wang,Han Chieh Kao,Ya Min Tsai,Hurng-Yi Wang,Chi Ling Hsieh,You Yu Lin,Pei-Jer Chen,Sui-Yuan Chang,Shiou-Hwei Yeh +11 more
TL;DR: In this paper, the impact of the D614G substitution on syncytium formation through enhanced furin-mediated S cleavage has been validated in S-expressing cells, and the antiviral effect through targeting furin protease is worthy of being investigated in proper animal models.
Journal ArticleDOI
Synergistic block of SARS-CoV-2 infection by combined drug inhibition of the host entry factors PIKfyve kinase and TMPRSS2 protease.
Alex J. B. Kreutzberger,Alex J. B. Kreutzberger,Anwesha Sanyal,Anwesha Sanyal,Ravi Ojha,Jesse D. Pyle,Olli Vapalahti,Giuseppe Balistreri,Tom Kirchhausen,Tom Kirchhausen +9 more
TL;DR: In this article, the authors combined apilimod with camostat mesylate or nafamostat meshesylate and found an unexpected ∼5- to 10-fold increase in their effectiveness to prevent SARS-CoV-2 infection.
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