Structural and Functional Analysis of the D614G SARS-CoV-2 Spike Protein Variant.
Leonid Yurkovetskiy,Xue Wang,Kristen E. Pascal,Christopher Tomkins-Tinch,Thomas Nyalile,Yetao Wang,Alina Baum,William E. Diehl,Ann Dauphin,Claudia Carbone,Kristen Veinotte,Shawn B. Egri,Stephen F. Schaffner,Stephen F. Schaffner,Jacob E. Lemieux,James B. Munro,Ashique Rafique,Abhi Barve,Pardis C. Sabeti,Christos A. Kyratsous,Natalya Dudkina,Kuang Shen,Jeremy Luban +22 more
TLDR
It is shown that D614G was more infectious than the ancestral form on human lung cells, colon cells, and on cells rendered permissive by ectopic expression of human ACE2 or of ACE2 orthologs from various mammals, including Chinese rufous horseshoe bat and Malayan pangolin.About:
This article is published in Cell.The article was published on 2020-10-29 and is currently open access. It has received 840 citations till now. The article focuses on the topics: Virion membrane & Protein structure.read more
Citations
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Cryo-EM Structures of SARS-CoV-2 Spike without and with ACE2 Reveal a pH-Dependent Switch to Mediate Endosomal Positioning of Receptor-Binding Domains.
Tongqing Zhou,Yaroslav Tsybovsky,Jason Gorman,Micah Rapp,Gabriele Cerutti,Gwo-Yu Chuang,Phinikoula S. Katsamba,Jared M. Sampson,Arne Schön,Jude Bimela,Jeffrey C. Boyington,Alexandra Nazzari,Adam S. Olia,Wei Shi,Mallika Sastry,Tyler Stephens,Jonathan Stuckey,I-Ting Teng,Pengfei Wang,Shuishu Wang,Baoshan Zhang,Richard A. Friesner,David D. Ho,John R. Mascola,Lawrence Shapiro,Lawrence Shapiro,Lawrence Shapiro,Peter D. Kwong,Peter D. Kwong +28 more
TL;DR: Insight is provided into how receptor interactions and endosomal pH alter RBD positioning and potentially facilitate immune evasion from RBD-up binding antibody.
Journal ArticleDOI
BNT162b2-elicited neutralization of B.1.617 and other SARS-CoV-2 variants.
Jianying Liu,Yang Liu,Hongjie Xia,Jing Zou,Scott C. Weaver,Kena A. Swanson,Hui Cai,Mark Cutler,David A. Cooper,Alexander Muik,Kathrin U. Jansen,Ugur Sahin,Xuping Xie,Philip R. Dormitzer,Pei Yong Shi +14 more
TL;DR: The authors showed that serum samples taken from twenty human volunteers, two or four weeks after their second dose of the BNT162b2 vaccine, neutralize engineered SARS-CoV-2 with a USA-WA1/2020 genetic background (a virus strain isolated in January 2020) and spike glycoproteins from the recently identified B.1.617.
Journal ArticleDOI
D614G Spike Mutation Increases SARS CoV-2 Susceptibility to Neutralization.
Drew Weissman,Mohamad-Gabriel Alameh,Thushan I de Silva,Paul Collini,Hailey Hornsby,Rebecca Brown,Celia C. LaBranche,Robert J. Edwards,Laura L. Sutherland,Sampa Santra,Katayoun Mansouri,Sophie M. C. Gobeil,Charlene McDanal,Norbert Pardi,Nick Hengartner,Paulo J.C. Lin,Ying Tam,Pamela A. Shaw,Mark G. Lewis,Carsten Boesler,Ugur Sahin,Priyamvada Acharya,Barton F. Haynes,Bette T. Korber,David C. Montefiori +24 more
TL;DR: Sera from Spike-immunized mice, nonhuman primates and humans were evaluated for neutralization of pseudoviruses bearing either D614 or G614 spike, and found the G614 pseudovirus was moderately more susceptible to neutralization.
Journal ArticleDOI
No evidence for increased transmissibility from recurrent mutations in SARS-CoV-2.
TL;DR: It is found that there is no evidence for significantly more transmissible lineages of SARS-CoV-2 due to recurrent mutations, and recurrent mutations currently in circulation appear to be evolutionary neutral and primarily induced by the human immune system via RNA editing, rather than being signatures of adaptation.
Journal ArticleDOI
Spread of a SARS-CoV-2 variant through Europe in the summer of 2020.
Emma B. Hodcroft,Emma B. Hodcroft,Emma B. Hodcroft,Moira Zuber,Sarah Nadeau,Sarah Nadeau,Timothy G. Vaughan,Timothy G. Vaughan,Katharine H.D. Crawford,Katharine H.D. Crawford,Christian L. Althaus,Martina L. Reichmuth,John E. Bowen,Alexandra C. Walls,Davide Corti,Jesse D. Bloom,Jesse D. Bloom,Jesse D. Bloom,David Veesler,David Mateo,Alberto Hernando,Iñaki Comas,Fernando González-Candelas,Tanja Stadler,Tanja Stadler,Richard A. Neher,Richard A. Neher +26 more
TL;DR: A novel SARS-CoV-2 variant, 20E (EU1) that emerged in Spain in early summer, and subsequently spread across Europe is reported in this article.
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