Structural and Functional Analysis of the D614G SARS-CoV-2 Spike Protein Variant.
Leonid Yurkovetskiy,Xue Wang,Kristen E. Pascal,Christopher Tomkins-Tinch,Thomas Nyalile,Yetao Wang,Alina Baum,William E. Diehl,Ann Dauphin,Claudia Carbone,Kristen Veinotte,Shawn B. Egri,Stephen F. Schaffner,Stephen F. Schaffner,Jacob E. Lemieux,James B. Munro,Ashique Rafique,Abhi Barve,Pardis C. Sabeti,Christos A. Kyratsous,Natalya Dudkina,Kuang Shen,Jeremy Luban +22 more
TLDR
It is shown that D614G was more infectious than the ancestral form on human lung cells, colon cells, and on cells rendered permissive by ectopic expression of human ACE2 or of ACE2 orthologs from various mammals, including Chinese rufous horseshoe bat and Malayan pangolin.About:
This article is published in Cell.The article was published on 2020-10-29 and is currently open access. It has received 840 citations till now. The article focuses on the topics: Virion membrane & Protein structure.read more
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A rigorous framework for detecting SARS-CoV-2 spike protein mutational ensemble from genomic and structural features.
Saman Fatihi,Saman Fatihi,Surabhi Rathore,Surabhi Rathore,Ankit K. Pathak,Deepanshi Gahlot,Deepanshi Gahlot,Mitali Mukerji,Mitali Mukerji,Nidhi Jatana,Lipi Thukral,Lipi Thukral +11 more
TL;DR: In this article, the authors temporally track 527,988 SARS-CoV-2 genomes and identify invariant and hypervariable regions within the spike protein and find that the maximum number of lineage-defining mutations were present in the N-terminal domain (NTD).
Journal ArticleDOI
Efficacy and safety of an inactivated whole-virion vaccine against COVID-19, QazCovid-in®, in healthy adults: A multicentre, randomised, single-blind, placebo-controlled phase 3 clinical trial with a 6-month follow-up
Berik Khairullin,Kunsulu Zakarya,Mukhit Orynbayev,Yergali Abduraimov,Markhabat Kassenov,Gulbanu Sarsenbayeva,Kulyaisan Sultankulova,Olga V. Chervyakova,Balzhan Myrzakhmetova,Aziz Nakhanov,Ainur Nurpeisova,Kuandyk Zhugunissov,Nurika Assanzhanova,Sergazy Nurabayev,Aslan Kerimbayev,Zakir Yershebulov,Yerbol Burashev,I. Kulmagambetov,Timur Davlyatshin,M. Sergeeva,Zhanna Buzitskaya,Marina Stukova,Lespek Kutumbetov +22 more
TL;DR: The QazCovid-in® vaccine was safe over the 6-month monitoring period after two intramuscular immunisations inducing only local short-lived adverse events as discussed by the authors .
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Effects of Spike Mutations in SARS-CoV-2 Variants of Concern on Human or Animal ACE2-Mediated Virus Entry and Neutralization
Yunjeong Kim,Natasha N. Gaudreault,David A. Meekins,Krishani Dinali Perera,Dashzeveg Bold,Jessie D. Trujillo,Igor Morozov,Chester McDowell,Kyeong-Ok Chang,Juergen A. Richt +9 more
TL;DR: In this paper, the effect of mutations in the SARS-CoV-2 Spike protein (S) has been investigated in nine different animal species to gain insights into the effects of VOC mutations on viral entry and antibody neutralization capability.
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Distinct shifts in site-specific glycosylation pattern of SARS-CoV-2 spike proteins associated with arising mutations in the D614G and Alpha variants.
Chu-Wei Kuo,Tzu-Jing Yang,Yu-Chun Chien,Pei-Yu Yu,Shang-Te Danny Hsu,Shang-Te Danny Hsu,Kay-Hooi Khoo,Kay-Hooi Khoo +7 more
TL;DR: In this paper, mass spectrometry-based site-specific glycosylation analyses of similarly produced spike proteins with and without the D614G and Alpha variant mutations reveal a significant shift in the processing state of N-glycans on one specific NTD site.
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Development of a High-Throughput Homogeneous AlphaLISA Drug Screening Assay for the Detection of SARS-CoV-2 Nucleocapsid
Kirill Gorshkov,Catherine Z. Chen,Miao Xu,Juan Carlos de la Torre,Luis Martinez-Sobrido,Thomas M. Moran,Wei Zheng +6 more
TL;DR: A homogeneous cell-based HTS system using AlphaLISA detection technology for the SARS-CoV-2 nucleocapsid protein (NP) with high sensitivity and a large dynamic range, which can be used for high-throughput screening of small molecules and biologics in the fight against the COVID-19 pandemic.
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