Structural and Functional Analysis of the D614G SARS-CoV-2 Spike Protein Variant.
Leonid Yurkovetskiy,Xue Wang,Kristen E. Pascal,Christopher Tomkins-Tinch,Thomas Nyalile,Yetao Wang,Alina Baum,William E. Diehl,Ann Dauphin,Claudia Carbone,Kristen Veinotte,Shawn B. Egri,Stephen F. Schaffner,Stephen F. Schaffner,Jacob E. Lemieux,James B. Munro,Ashique Rafique,Abhi Barve,Pardis C. Sabeti,Christos A. Kyratsous,Natalya Dudkina,Kuang Shen,Jeremy Luban +22 more
TLDR
It is shown that D614G was more infectious than the ancestral form on human lung cells, colon cells, and on cells rendered permissive by ectopic expression of human ACE2 or of ACE2 orthologs from various mammals, including Chinese rufous horseshoe bat and Malayan pangolin.About:
This article is published in Cell.The article was published on 2020-10-29 and is currently open access. It has received 840 citations till now. The article focuses on the topics: Virion membrane & Protein structure.read more
Citations
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Structural and antigenic variations in the spike protein of emerging SARS-CoV-2 variants
TL;DR: Escape maps of the epitope landscape of nAbs on the spike protein are a valuable tool to predict SARS-CoV-2 fitness and in conjunction with the structures of the spike-nAb complex, they can be utilized to facilitate the rational design of escape-resistant antibody therapeutics and vaccines.
Journal ArticleDOI
Spike glycoprotein and host cell determinants of SARS-CoV-2 entry and cytopathic effects.
Hanh T. Nguyen,Shijian Zhang,Qian Wang,Saumya Anang,Jia Wang,Haitao Ding,John C. Kappes,John C. Kappes,Joseph Sodroski +8 more
TL;DR: Key features of the spike glycoprotein, including the furin cleavage site and the D614G natural mutation, that modulate viral cytopathic effects, infectivity, and sensitivity to inhibition are identified.
Journal ArticleDOI
Structural insight into SARS-CoV-2 neutralizing antibodies and modulation of syncytia.
Daniel Asarnow,Bei Wang,Wen-Hsin Lee,Yuanyu Hu,Ching-wen Huang,Bryan Faust,Patricia M. L. Ng,Eve Ngoh,Markus Bohn,David Bulkley,Andrés Pizzorno,Beatrice Ary,Hwee Ching Tan,Chia Yin Lee,Rabiatul Adawiyah Minhat,Olivier Terrier,Mun Kuen Soh,Frannie Jiuyi Teo,Yvonne Yee Chin Yeap,Shirley Gek Kheng Seah,Conrad E.Z. Chan,Emily Connelly,Nicholas J. Young,Sebastian Maurer-Stroh,Sebastian Maurer-Stroh,Laurent Rénia,Brendon J. Hanson,Manuel Rosa-Calatrava,Aashish Manglik,Yifan Cheng,Yifan Cheng,Charles S. Craik,Cheng-I Wang +32 more
TL;DR: In this article, a series of antibodies that potently block ACE2 binding but exhibit divergent neutralization efficacy against the live SARS-CoV-2 was discovered, and these neutralizing antibodies can inhibit or enhance Spike-mediated membrane fusion and formation of syncytia, which are associated with chronic tissue damage in individuals with COVID-19.
Journal ArticleDOI
Structural Evaluation of the Spike Glycoprotein Variants on SARS-CoV-2 Transmission and Immune Evasion.
TL;DR: In this paper, the more important variants of SARS-CoV-2, which include cluster 5, lineages B.1.7 (Alpha variant), P.1 (B.28/Gamma), B. 1.427/B1.41/B.617.
Journal ArticleDOI
The Mechanism and Consequences of SARS-CoV-2 Spike-Mediated Fusion and Syncytia Formation.
Maaran Michael Rajah,Maaran Michael Rajah,Ana Cristina Gallego-Hernández,Annie Bernier,Julian Buchrieser,Olivier Schwartz,Olivier Schwartz +6 more
TL;DR: In this article, the authors discuss the molecular determinants of S mediated fusion and the antiviral innate immunity components that counteract syncytia formation in SARS-CoV-2 infection and pathology.
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