Journal ArticleDOI
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
TLDR
It is shown that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location, and it is proposed that this RNA is called “exosomal shuttle RNA” (esRNA).Abstract:
Exosomes are vesicles of endocytic origin released by many cells. These vesicles can mediate communication between cells, facilitating processes such as antigen presentation. Here, we show that exosomes from a mouse and a human mast cell line (MC/9 and HMC-1, respectively), as well as primary bone marrow-derived mouse mast cells, contain RNA. Microarray assessments revealed the presence of mRNA from approximately 1300 genes, many of which are not present in the cytoplasm of the donor cell. In vitro translation proved that the exosome mRNAs were functional. Quality control RNA analysis of total RNA derived from exosomes also revealed presence of small RNAs, including microRNAs. The RNA from mast cell exosomes is transferable to other mouse and human mast cells. After transfer of mouse exosomal RNA to human mast cells, new mouse proteins were found in the recipient cells, indicating that transferred exosomal mRNA can be translated after entering another cell. In summary, we show that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location. We propose that this RNA is called "exosomal shuttle RNA" (esRNA).read more
Citations
More filters
Journal ArticleDOI
CAF secreted miR-522 suppresses ferroptosis and promotes acquired chemo-resistance in gastric cancer.
Haiyang Zhang,Ting Deng,Rui Liu,Tao Ning,Haiou Yang,Dongying Liu,Qiumo Zhang,Dan Lin,Shaohua Ge,Ming Bai,Xinyi Wang,Le Zhang,Hongli Li,Yuchong Yang,Zhi Ji,Hailong Wang,Guoguang Ying,Yi Ba +17 more
TL;DR: It is demonstrated that CAFs secrete exosomal miR-522 to inhibit ferroptosis in cancer cells by targeting ALOX15 and blocking lipid-ROS accumulation, which results in decreased chemo-sensitivity.
Journal ArticleDOI
A Comparative Study of Serum Exosome Isolation Using Differential Ultracentrifugation and Three Commercial Reagents
Inas Helwa,Jingwen Cai,Michelle Drewry,Arthur D. Zimmerman,Michael B. Dinkins,Mariam Lotfy Khaled,Mutsa Seremwe,W. Michael Dismuke,Erhard Bieberich,W. Daniel Stamer,Mark W. Hamrick,Yutao Liu,Yutao Liu +12 more
TL;DR: Comparing the exosomes extracted by three different exosome isolation kits and differential ultracentrifugation using six different volumes of a non-cancerous human serum shows that three kits are viable alternatives to UC, even when limited amounts of biological samples are available.
Journal ArticleDOI
Exosomes: Novel Biomarkers for Clinical Diagnosis
Jin Lin,Jing Li,Bo Huang,Jing Liu,Xin Chen,Xi-Min Chen,Yan-Mei Xu,Lin-Feng Huang,Xiao-Zhong Wang +8 more
TL;DR: This minireview summarizes recent advances in the research of exosomal biomarkers and their potential application in clinical diagnostics and provides a brief overview of the formation, function, and isolation ofExosomes.
Journal ArticleDOI
Mesenchymal stem cell exosomes.
TL;DR: It is proposed here that MSC exosomes function as an extension of MSC's biological role as tissue stromal support cells, enabling cells to recover critical cellular functions and begin repair and regeneration.
Journal ArticleDOI
Exosomes: small vesicles participating in intercellular communication.
Anna-Kristin Ludwig,Bernd Giebel +1 more
TL;DR: Exosomes are molecular complex intercellular signaling organelles with multiple functions, which appear as promising new tools for the clinical diagnostics and potentially for novel therapeutic strategies.
References
More filters
Journal ArticleDOI
Combinatorial microRNA target predictions.
Azra Krek,Dominic Grün,Matthew N. Poy,Rachel Wolf,Lauren Rosenberg,Eric J Epstein,Philip MacMenamin,Isabelle da Piedade,Kristin C. Gunsalus,Markus Stoffel,Nikolaus Rajewsky +10 more
TL;DR: PicTar, a computational method for identifying common targets of micro RNAs, is presented and widespread coordinate control executed by microRNAs is suggested, thus providing evidence for coordinate microRNA control in mammals.
Journal ArticleDOI
B lymphocytes secrete antigen-presenting vesicles.
Graça Raposo,Hans W. Nijman,Willem Stoorvogel,R Liejendekker,Clifford V. Harding,Cornelis J. M. Melief,Hans J. Geuze +6 more
TL;DR: It is demonstrated by immunoelectron microscopy that the limiting membrane of MIICs can fuse directly with the plasma membrane, resulting in release from the cells of internal MHC class II-containing vesicles, suggesting a role for exosomes in antigen presentation in vivo.
Journal ArticleDOI
Identification and proteomic profiling of exosomes in human urine
TL;DR: The results indicate that exosome isolation may provide an efficient first step in biomarker discovery in urine and identify numerous protein components of MVBs and of the endosomal pathway in general.
Journal ArticleDOI
Fate of the transferrin receptor during maturation of sheep reticulocytes in vitro: Selective externalization of the receptor
Bin-Tao Pan,Rose M. Johnstone +1 more
TL;DR: The fate of the transferrin receptor during in vitro maturation of sheep reticulocytes has been followed using FITC- and 125I-labeled anti-transferrin-receptor antibodies and it can be shown that at 0 degree C or in phosphate-buffered saline the rate of vesicle release is less than that at 37 degrees C in culture medium.
Journal ArticleDOI
Embryonic stem cell-derived microvesicles reprogram hematopoietic progenitors: evidence for horizontal transfer of mRNA and protein delivery.
Janina Ratajczak,Katarzyna Miekus,Magdalena Kucia,Jin Zhang,Ryan Reca,Petr Dvorak,Mariusz Z. Ratajczak +6 more
TL;DR: ES-MV isolated from murine ES cells in serum-free cultures significantly enhanced survival and improved expansion of murine HPC, and upregulated the expression of early pluripotent and early hematopoietic stem cells in these cells.
Related Papers (5)
Extracellular vesicles: exosomes, microvesicles, and friends.
Circulating microRNAs as stable blood-based markers for cancer detection
Patrick S. Mitchell,Rachael K. Parkin,Evan M. Kroh,Brian R. Fritz,Brian R. Fritz,Stacia K. Wyman,Era L. Pogosova-Agadjanyan,Amelia Peterson,Jennifer Noteboom,Kathy O'Briant,April Allen,Daniel W. Lin,Daniel W. Lin,Daniel W. Lin,Nicole Urban,Charles W. Drescher,Beatrice S. Knudsen,Derek L. Stirewalt,Robert Gentleman,Robert L. Vessella,Robert L. Vessella,Peter S. Nelson,Daniel Martin,Daniel Martin,Muneesh Tewari +24 more