Journal ArticleDOI
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
TLDR
It is shown that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location, and it is proposed that this RNA is called “exosomal shuttle RNA” (esRNA).Abstract:
Exosomes are vesicles of endocytic origin released by many cells. These vesicles can mediate communication between cells, facilitating processes such as antigen presentation. Here, we show that exosomes from a mouse and a human mast cell line (MC/9 and HMC-1, respectively), as well as primary bone marrow-derived mouse mast cells, contain RNA. Microarray assessments revealed the presence of mRNA from approximately 1300 genes, many of which are not present in the cytoplasm of the donor cell. In vitro translation proved that the exosome mRNAs were functional. Quality control RNA analysis of total RNA derived from exosomes also revealed presence of small RNAs, including microRNAs. The RNA from mast cell exosomes is transferable to other mouse and human mast cells. After transfer of mouse exosomal RNA to human mast cells, new mouse proteins were found in the recipient cells, indicating that transferred exosomal mRNA can be translated after entering another cell. In summary, we show that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location. We propose that this RNA is called "exosomal shuttle RNA" (esRNA).read more
Citations
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Circulating microRNA expression is reduced in chronic kidney disease
Calida S. Neal,Michael Michael,Letitia K. Pimlott,Tuck Y. Yong,Jordan Y. Z. Li,Jonathan M. Gleadle +5 more
TL;DR: In patients with severe chronic renal failure, circulating levels of total and specific miRNAs are reduced in comparison to patients with mild renal impairment or normal renal function.
Journal ArticleDOI
Aspirin and Cancer
Paola Patrignani,Carlo Patrono +1 more
TL;DR: The evidence supporting a chemopreventive effect of aspirin is reviewed, its potential mechanism(s) of action is discussed, and a conceptual framework for assessing current guidelines in the light of ongoing studies is provided.
Journal ArticleDOI
Expression profiling of cancerous and normal breast tissues identifies microRNAs that are differentially expressed in serum from patients with (metastatic) breast cancer and healthy volunteers
Eleni van Schooneveld,Maartje C.A. Wouters,Ilse Van der Auwera,Dieter Peeters,H. Wildiers,Peter A. van Dam,Ignace Vergote,Peter B. Vermeulen,Luc Dirix,Steven Van Laere +9 more
TL;DR: This study identified and tested a set of putative biomarkers of breast cancer and demonstrated that altered levels of these miRNAs in serum from patients with breast cancer are particularly associated with the presence of metastatic disease.
Journal ArticleDOI
Tumor-cell-derived microvesicles as carriers of molecular information in cancer
TL;DR: Because of their stability, exosomes and microvesicles can be retrieved in bodily fluids as biomarkers for cancer detection and monitoring and offer a range of molecular targets for controlling cell–cell interactions during invasion and metastasis.
Journal ArticleDOI
Extracellular vesicles as emerging intercellular communicasomes
TL;DR: This review focuses on the components, functions, and therapeutic and diagnostic potential of extracellular vesicles under various pathophysiological conditions.
References
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Journal ArticleDOI
Combinatorial microRNA target predictions.
Azra Krek,Dominic Grün,Matthew N. Poy,Rachel Wolf,Lauren Rosenberg,Eric J Epstein,Philip MacMenamin,Isabelle da Piedade,Kristin C. Gunsalus,Markus Stoffel,Nikolaus Rajewsky +10 more
TL;DR: PicTar, a computational method for identifying common targets of micro RNAs, is presented and widespread coordinate control executed by microRNAs is suggested, thus providing evidence for coordinate microRNA control in mammals.
Journal ArticleDOI
B lymphocytes secrete antigen-presenting vesicles.
Graça Raposo,Hans W. Nijman,Willem Stoorvogel,R Liejendekker,Clifford V. Harding,Cornelis J. M. Melief,Hans J. Geuze +6 more
TL;DR: It is demonstrated by immunoelectron microscopy that the limiting membrane of MIICs can fuse directly with the plasma membrane, resulting in release from the cells of internal MHC class II-containing vesicles, suggesting a role for exosomes in antigen presentation in vivo.
Journal ArticleDOI
Identification and proteomic profiling of exosomes in human urine
TL;DR: The results indicate that exosome isolation may provide an efficient first step in biomarker discovery in urine and identify numerous protein components of MVBs and of the endosomal pathway in general.
Journal ArticleDOI
Fate of the transferrin receptor during maturation of sheep reticulocytes in vitro: Selective externalization of the receptor
Bin-Tao Pan,Rose M. Johnstone +1 more
TL;DR: The fate of the transferrin receptor during in vitro maturation of sheep reticulocytes has been followed using FITC- and 125I-labeled anti-transferrin-receptor antibodies and it can be shown that at 0 degree C or in phosphate-buffered saline the rate of vesicle release is less than that at 37 degrees C in culture medium.
Journal ArticleDOI
Embryonic stem cell-derived microvesicles reprogram hematopoietic progenitors: evidence for horizontal transfer of mRNA and protein delivery.
Janina Ratajczak,Katarzyna Miekus,Magdalena Kucia,Jin Zhang,Ryan Reca,Petr Dvorak,Mariusz Z. Ratajczak +6 more
TL;DR: ES-MV isolated from murine ES cells in serum-free cultures significantly enhanced survival and improved expansion of murine HPC, and upregulated the expression of early pluripotent and early hematopoietic stem cells in these cells.
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