Journal ArticleDOI
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
TLDR
It is shown that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location, and it is proposed that this RNA is called “exosomal shuttle RNA” (esRNA).Abstract:
Exosomes are vesicles of endocytic origin released by many cells. These vesicles can mediate communication between cells, facilitating processes such as antigen presentation. Here, we show that exosomes from a mouse and a human mast cell line (MC/9 and HMC-1, respectively), as well as primary bone marrow-derived mouse mast cells, contain RNA. Microarray assessments revealed the presence of mRNA from approximately 1300 genes, many of which are not present in the cytoplasm of the donor cell. In vitro translation proved that the exosome mRNAs were functional. Quality control RNA analysis of total RNA derived from exosomes also revealed presence of small RNAs, including microRNAs. The RNA from mast cell exosomes is transferable to other mouse and human mast cells. After transfer of mouse exosomal RNA to human mast cells, new mouse proteins were found in the recipient cells, indicating that transferred exosomal mRNA can be translated after entering another cell. In summary, we show that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location. We propose that this RNA is called "exosomal shuttle RNA" (esRNA).read more
Citations
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Journal ArticleDOI
Sorting Mechanisms for MicroRNAs into Extracellular Vesicles and Their Associated Diseases.
Michael Groot,Heedoo Lee +1 more
TL;DR: The mechanisms whereby cells selectively sort miRNA into EVs are overviewed and disease states where EV-miRNAs become dysregulated are outlined, shedding light on the potential role of selective sorting in pathogenesis.
Journal ArticleDOI
Mesenchymal stem cell derived-exosomes: a modern approach in translational medicine.
TL;DR: In comparison with their donor cells, MSC-derived exosomes offer more stable entities and diminished safety risks regarding the administration of live cells, e.g. microvasculature occlusion risk.
Journal ArticleDOI
The exosomes in tumor immunity
Yanfang Liu,Yan Gu,Xuetao Cao +2 more
TL;DR: This review focuses on exosome-mediated Immunosuppression via inhibition of antitumor responses elicited by immune cells and induction of immunosuppressive or regulatory cell populations (MDSCs, Tregs and Bregs), and the current application of DC-derived and modified tumor-derived exosomes as tumor vaccines.
Journal ArticleDOI
Serum microRNA-1 and microRNA-122 are prognostic markers in patients with hepatocellular carcinoma
Verena Köberle,Bernd Kronenberger,Thomas Pleli,Jörg Trojan,Esther Imelmann,Jan Peveling-Oberhag,Martin-Walter Welker,Mohammed Elhendawy,Stefan Zeuzem,Albrecht Piiper,Oliver Waidmann +10 more
TL;DR: The data indicate that serum miR-1 is a new independent parameter of OS in HCC patients and may therefore improve the predictive value of classical HCC staging scores.
Journal ArticleDOI
Circulating miR-30a, miR-126 and let-7b as biomarker for ischemic stroke in humans
Guangwen Long,Feng Wang,Huaping Li,Zhongwei Yin,Chaugai Sandip,Yan Lou,Yan Wang,Chen Chen,Dao Wen Wang +8 more
TL;DR: Data suggest thatmiR-30a, miR-126 and let-7b might be useful biomarkers for ischemic stroke in humans.
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