Journal ArticleDOI
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
TLDR
It is shown that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location, and it is proposed that this RNA is called “exosomal shuttle RNA” (esRNA).Abstract:
Exosomes are vesicles of endocytic origin released by many cells. These vesicles can mediate communication between cells, facilitating processes such as antigen presentation. Here, we show that exosomes from a mouse and a human mast cell line (MC/9 and HMC-1, respectively), as well as primary bone marrow-derived mouse mast cells, contain RNA. Microarray assessments revealed the presence of mRNA from approximately 1300 genes, many of which are not present in the cytoplasm of the donor cell. In vitro translation proved that the exosome mRNAs were functional. Quality control RNA analysis of total RNA derived from exosomes also revealed presence of small RNAs, including microRNAs. The RNA from mast cell exosomes is transferable to other mouse and human mast cells. After transfer of mouse exosomal RNA to human mast cells, new mouse proteins were found in the recipient cells, indicating that transferred exosomal mRNA can be translated after entering another cell. In summary, we show that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location. We propose that this RNA is called "exosomal shuttle RNA" (esRNA).read more
Citations
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Journal ArticleDOI
Imaging extracellular vesicles: current and emerging methods.
TL;DR: Current and emerging strategies for EV imaging for prospective studies are reviewed, covering current and emerging methods to visualize and study their spatiotemporal properties.
Journal ArticleDOI
Modulation of hypoxia-signaling pathways by extracellular linc-RoR
TL;DR: These studies show that linc-RoR is a hypoxia-responsive lncRNA that is functionally linked to hypoxian signaling in HCC through a miR-145–HIF-1&agr; signaling module, and identifies a mechanistic role for the extracellular transfer of linc -RoR in intercellular signaling to promote cell survival during hypoxic stress.
Journal ArticleDOI
Exosome-mediated delivery of miR-9 induces cancer-associated fibroblast-like properties in human breast fibroblasts.
S. Baroni,Sandra Romero-Cordoba,Ilaria Plantamura,Matteo Dugo,Elvira D'Ippolito,Alessandra Cataldo,Giulia Cosentino,V Angeloni,A Rossini,Maria Grazia Daidone,Marilena V. Iorio +10 more
TL;DR: It is shown that miR-9, upregulated in various breast cancer cell lines and identified as pro-metastatic miRNA, affects the properties of human breast fibroblasts, enhancing the switch to CAF phenotype, thus contributing to tumor growth.
Journal ArticleDOI
Proteomic analysis of two types of exosomes in human whole saliva.
Yuko Ogawa,Yuri Miura,Akira Harazono,Masami Kanai-Azuma,Yoshihiro Akimoto,Hayato Kawakami,Teruhide Yamaguchi,Tosifusa Toda,Tamao Endo,Masayoshi Tsubuki,Ryohei Yanoshita +10 more
TL;DR: Human whole saliva exosomes might participate in the catabolism of bioactive peptides and play a regulatory role in local immune defense in the oral cavity.
Journal ArticleDOI
Osteoclast-derived microRNA-containing exosomes selectively inhibit osteoblast activity.
Weijia Sun,Chenyang Zhao,Yuheng Li,Liang Wang,Guangjun Nie,Jiang Peng,Aiyuan Wang,Pengfei Zhang,Weiming Tian,Qi Li,Jinping Song,Cheng Wang,Xiaolong Xu,Yanhua Tian,Dingsheng Zhao,Zi Xu,Guohui Zhong,Bingxing Han,Shukuan Ling,Yan Zhong Chang,Yingxian Li +20 more
TL;DR: It is reported that osteoclasts secrete microRNA-enriched exosomes, by which miR-214 is transferred into osteoblasts to inhibit their function, suggesting that exosome-mediated transfer of microRNA plays an important role in the regulation of osteoblast activity.
References
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Journal ArticleDOI
Combinatorial microRNA target predictions.
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Journal ArticleDOI
Identification and proteomic profiling of exosomes in human urine
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Journal ArticleDOI
Fate of the transferrin receptor during maturation of sheep reticulocytes in vitro: Selective externalization of the receptor
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TL;DR: The fate of the transferrin receptor during in vitro maturation of sheep reticulocytes has been followed using FITC- and 125I-labeled anti-transferrin-receptor antibodies and it can be shown that at 0 degree C or in phosphate-buffered saline the rate of vesicle release is less than that at 37 degrees C in culture medium.
Journal ArticleDOI
Embryonic stem cell-derived microvesicles reprogram hematopoietic progenitors: evidence for horizontal transfer of mRNA and protein delivery.
Janina Ratajczak,Katarzyna Miekus,Magdalena Kucia,Jin Zhang,Ryan Reca,Petr Dvorak,Mariusz Z. Ratajczak +6 more
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