Journal ArticleDOI
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
TLDR
It is shown that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location, and it is proposed that this RNA is called “exosomal shuttle RNA” (esRNA).Abstract:
Exosomes are vesicles of endocytic origin released by many cells. These vesicles can mediate communication between cells, facilitating processes such as antigen presentation. Here, we show that exosomes from a mouse and a human mast cell line (MC/9 and HMC-1, respectively), as well as primary bone marrow-derived mouse mast cells, contain RNA. Microarray assessments revealed the presence of mRNA from approximately 1300 genes, many of which are not present in the cytoplasm of the donor cell. In vitro translation proved that the exosome mRNAs were functional. Quality control RNA analysis of total RNA derived from exosomes also revealed presence of small RNAs, including microRNAs. The RNA from mast cell exosomes is transferable to other mouse and human mast cells. After transfer of mouse exosomal RNA to human mast cells, new mouse proteins were found in the recipient cells, indicating that transferred exosomal mRNA can be translated after entering another cell. In summary, we show that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location. We propose that this RNA is called "exosomal shuttle RNA" (esRNA).read more
Citations
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Serum microRNA expression profile: miR-1246 as a novel diagnostic and prognostic biomarker for oesophageal squamous cell carcinoma
Nobuyoshi Takeshita,Isamu Hoshino,Mikito Mori,Yasunori Akutsu,Naoyuki Hanari,Yasuo Yoneyama,Norimasa Ikeda,Yuka Isozaki,Tetsurou Maruyama,Naoki Akanuma,Aki Komatsu,M Jitsukawa,Hiromasa Matsubara +12 more
TL;DR: These data support the contention that serum miR-1246 has strong potential as a novel diagnostic and prognostic biomarker in ESCC, and its releasing mechanism is selective and independent of tissue miRNA abundance.
Journal ArticleDOI
Analysis of exosome release and its prognostic value in human colorectal cancer
Jose M. Silva,Vanesa García,Marta Rodríguez,M. Compte,E. Cisneros,P. Veguillas,José María Franco García,Gemma Domínguez,Y. Campos-Martin,J. Cuevas,C. Pena,Mercedes Herrera,Raquel Diaz,N. Mohammed,Félix Bonilla +14 more
TL;DR: Although it was not suggested that the TP53/TSAP6 pathway regulates the release of exosomes into the plasma of colorectal cancer patients, the level of circulating exosome may be used as a tumor indicator, because it correlates with poor prognosis parameters and shorter survival.
Journal ArticleDOI
Tumor-derived exosomes regulate expression of immune function-related genes in human T cell subsets
TL;DR: Changes in the gene profiles of primary human T lymphocytes exposed in vitro to exosomes were evaluated and Treg were more sensitive to TEX-mediated effects than other T cell subsets.
Journal ArticleDOI
Exosomes and Cancer: A Newly Described Pathway of Immune Suppression
TL;DR: The effects of exosomes on the development and progression of cancers, with an emphasis on suppression of immune surveillance, is described and potential uses ofExosomes clinically, in the development of vaccines, in targeting tumors, and in diagnosis and/or early detection are discussed.
Journal ArticleDOI
Mechanism of Evenness Interrupted (Evi)-Exosome Release at Synaptic Boutons
Kate Koles,John J. Nunnari,Ceren Korkut,Romina Barria,Cassandra Brewer,Yihang Li,John D. Leszyk,Bing Zhang,Vivian Budnik +8 more
TL;DR: This work established an in vivo system to elucidate the mechanisms of exosomal release in the nervous system and identified two proteins, Rab11 and Syntaxin 1A (Syx1A), that were required for Evi vesicle release.
References
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Journal ArticleDOI
Combinatorial microRNA target predictions.
Azra Krek,Dominic Grün,Matthew N. Poy,Rachel Wolf,Lauren Rosenberg,Eric J Epstein,Philip MacMenamin,Isabelle da Piedade,Kristin C. Gunsalus,Markus Stoffel,Nikolaus Rajewsky +10 more
TL;DR: PicTar, a computational method for identifying common targets of micro RNAs, is presented and widespread coordinate control executed by microRNAs is suggested, thus providing evidence for coordinate microRNA control in mammals.
Journal ArticleDOI
B lymphocytes secrete antigen-presenting vesicles.
Graça Raposo,Hans W. Nijman,Willem Stoorvogel,R Liejendekker,Clifford V. Harding,Cornelis J. M. Melief,Hans J. Geuze +6 more
TL;DR: It is demonstrated by immunoelectron microscopy that the limiting membrane of MIICs can fuse directly with the plasma membrane, resulting in release from the cells of internal MHC class II-containing vesicles, suggesting a role for exosomes in antigen presentation in vivo.
Journal ArticleDOI
Identification and proteomic profiling of exosomes in human urine
TL;DR: The results indicate that exosome isolation may provide an efficient first step in biomarker discovery in urine and identify numerous protein components of MVBs and of the endosomal pathway in general.
Journal ArticleDOI
Fate of the transferrin receptor during maturation of sheep reticulocytes in vitro: Selective externalization of the receptor
Bin-Tao Pan,Rose M. Johnstone +1 more
TL;DR: The fate of the transferrin receptor during in vitro maturation of sheep reticulocytes has been followed using FITC- and 125I-labeled anti-transferrin-receptor antibodies and it can be shown that at 0 degree C or in phosphate-buffered saline the rate of vesicle release is less than that at 37 degrees C in culture medium.
Journal ArticleDOI
Embryonic stem cell-derived microvesicles reprogram hematopoietic progenitors: evidence for horizontal transfer of mRNA and protein delivery.
Janina Ratajczak,Katarzyna Miekus,Magdalena Kucia,Jin Zhang,Ryan Reca,Petr Dvorak,Mariusz Z. Ratajczak +6 more
TL;DR: ES-MV isolated from murine ES cells in serum-free cultures significantly enhanced survival and improved expansion of murine HPC, and upregulated the expression of early pluripotent and early hematopoietic stem cells in these cells.
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