Journal ArticleDOI
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
TLDR
It is shown that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location, and it is proposed that this RNA is called “exosomal shuttle RNA” (esRNA).Abstract:
Exosomes are vesicles of endocytic origin released by many cells. These vesicles can mediate communication between cells, facilitating processes such as antigen presentation. Here, we show that exosomes from a mouse and a human mast cell line (MC/9 and HMC-1, respectively), as well as primary bone marrow-derived mouse mast cells, contain RNA. Microarray assessments revealed the presence of mRNA from approximately 1300 genes, many of which are not present in the cytoplasm of the donor cell. In vitro translation proved that the exosome mRNAs were functional. Quality control RNA analysis of total RNA derived from exosomes also revealed presence of small RNAs, including microRNAs. The RNA from mast cell exosomes is transferable to other mouse and human mast cells. After transfer of mouse exosomal RNA to human mast cells, new mouse proteins were found in the recipient cells, indicating that transferred exosomal mRNA can be translated after entering another cell. In summary, we show that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location. We propose that this RNA is called "exosomal shuttle RNA" (esRNA).read more
Citations
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Journal ArticleDOI
Dendritic Cell-Derived Exosomes for Cancer Immunotherapy: What's Next?
Sophie Viaud,Clotilde Théry,Stéphanie Ploix,Thomas Tursz,Valérie Lapierre,Olivier Lantz,Laurence Zitvogel,Nathalie Chaput +7 more
TL;DR: This review focuses on the preclinical studies exploiting the immunogenicity of dendritic cell-derived exosomes (Dex) and will elaborate on the past and future vaccination trials conducted using Dex strategy in melanoma and non-small cell lung cancer patients.
Journal ArticleDOI
The stem cell secretome and its role in brain repair.
Denise Drago,Chiara Cossetti,Chiara Cossetti,Nunzio Iraci,Nunzio Iraci,Edoardo Gaude,Giovanna Musco,Angela Bachi,Stefano Pluchino,Stefano Pluchino +9 more
TL;DR: The stem cell secretome (SCS) includes cytokines, chemokines and growth factors, and has gained increasing attention in recent years because of its multiple implications for the repair, restoration or regeneration of injured tissues.
Journal ArticleDOI
The expression profile of C19MC microRNAs in primary human trophoblast cells and exosomes
Rogier B. Donker,Jean-Francois Mouillet,Tianjiao Chu,Carl A. Hubel,Donna B. Stolz,Adrian E. Morelli,Yoel Sadovsky +6 more
TL;DR: The data define the relative expression levels of C19MC miRNAs in trophoblasts and exosomes, and suggest that C19 MC miRNAAs function in placental-maternal signaling, suggesting that the largest gene cluster of human microRNAs (miRNAs) is exclusively expressed in the placenta and in undifferentiated cells.
Journal ArticleDOI
A small noncoding RNA signature found in exosomes of GBM patient serum as a diagnostic tool
Lorea Manterola,Elizabeth Guruceaga,Jaime Gállego Pérez-Larraya,Marisol Gonzalez-Huarriz,Patricia Jauregui,Sonia Tejada,Ricardo Díez-Valle,Victor Segura,Nicolás Samprón,Cristina Barrena,Irune Ruiz,Amaia Agirre,Angel Ayuso,Javier Rodríguez,Alvaro Gonzalez,Enric Xipell,Ander Matheu,Adolfo López de Munain,Teresa Tuñón,Idoya Zazpe,Jesus Garcia-Foncillas,Sophie Paris,Jean Yves Delattre,Marta M. Alonso +23 more
TL;DR: A small noncoding RNA signature in microvesicles isolated from GBM patient serum is uncovered that could be used as a fast and reliable differential diagnostic biomarker.
Journal ArticleDOI
Exosomal miRNAs as cancer biomarkers and therapeutic targets
Arron Thind,Clive Wilson +1 more
TL;DR: If ex-miRNA is to be employed in novel non-invasive diagnostic approaches and as a therapeutic target in cancer, two further advances are necessary: in methods to isolate and detect ex- miRNA, and a better understanding of their biogenesis and functions in tumour-cell communication.
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