Journal ArticleDOI
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
TLDR
It is shown that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location, and it is proposed that this RNA is called “exosomal shuttle RNA” (esRNA).Abstract:
Exosomes are vesicles of endocytic origin released by many cells. These vesicles can mediate communication between cells, facilitating processes such as antigen presentation. Here, we show that exosomes from a mouse and a human mast cell line (MC/9 and HMC-1, respectively), as well as primary bone marrow-derived mouse mast cells, contain RNA. Microarray assessments revealed the presence of mRNA from approximately 1300 genes, many of which are not present in the cytoplasm of the donor cell. In vitro translation proved that the exosome mRNAs were functional. Quality control RNA analysis of total RNA derived from exosomes also revealed presence of small RNAs, including microRNAs. The RNA from mast cell exosomes is transferable to other mouse and human mast cells. After transfer of mouse exosomal RNA to human mast cells, new mouse proteins were found in the recipient cells, indicating that transferred exosomal mRNA can be translated after entering another cell. In summary, we show that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location. We propose that this RNA is called "exosomal shuttle RNA" (esRNA).read more
Citations
More filters
Journal ArticleDOI
A visible and colorimetric aptasensor based on DNA-capped single-walled carbon nanotubes for detection of exosomes.
Yaokun Xia,Meng-Meng Liu,Liangliang Wang,An Yan,Wenhui He,Mei Chen,Jianming Lan,Jiaoxing Xu,Lunhui Guan,Jinghua Chen +9 more
TL;DR: A visible and simple approach detecting exosomes by integrating single-walled carbon nanotubes that being excellent water solubility (s-SWCNTs) and aptamer and this proposed colorimetric aptasensor can be universally applicable for the detection of other targets by simple change the aptamer.
Journal ArticleDOI
Enhanced Cardioprotection by Human Endometrium Mesenchymal Stem Cells Driven by Exosomal MicroRNA-21
Kan Wang,Zhi Jiang,Keith A. Webster,Jinghai Chen,Hengxun Hu,Yu Zhou,Jing Zhao,Lihan Wang,Yingchao Wang,Zhiwei Zhong,Cheng Ni,Qingju Li,Charlie Xiang,Ling Zhang,Rongrong Wu,Wei Zhu,Hong Yu,Xinyang Hu,Jian-an Wang +18 more
TL;DR: The results of the present study confirm the superior cardioprotection by EnMSCs relative to BMMSCs or AdMSCs and implicates miR‐21 as a potential mediator of EnMSC therapy by enhancing cell survival through the PTEN/Akt pathway.
Journal ArticleDOI
STAT3-regulated exosomal miR-21 promotes angiogenesis and is involved in neoplastic processes of transformed human bronchial epithelial cells
Yi Liu,Fei Luo,Bairu Wang,Huiqiao Li,Yuan Xu,Xinlu Liu,Le Shi,Xiaolin Lu,Wenchao Xu,Lu Lu,Yu Qin,Quanyong Xiang,Qizhan Liu +12 more
TL;DR: Exosomal miR-21 in exosomes leads to STAT3 activation, which increases VEGF levels in recipient cells, a process involved in angiogenesis and malignant transformation of HBE cells, providing a new perspective for intervention strategies to prevent carcinogenesis of lung cancer.
Journal ArticleDOI
Exosome and mesenchymal stem cell cross-talk in the tumor microenvironment.
TL;DR: Although MSCs have a potential to exert anti-tumor activities, they largely provide service to the tumor using the multidirectional communication system established by exosomes in the TME.
Journal ArticleDOI
New insights into the function of Rab GTPases in the context of exosomal secretion.
Lionel Blanc,Michel Vidal +1 more
TL;DR: The possibility that different routes regulated by specific Rab GTPases might produce exosomes having various biologic functions is discussed.
References
More filters
Journal ArticleDOI
Combinatorial microRNA target predictions.
Azra Krek,Dominic Grün,Matthew N. Poy,Rachel Wolf,Lauren Rosenberg,Eric J Epstein,Philip MacMenamin,Isabelle da Piedade,Kristin C. Gunsalus,Markus Stoffel,Nikolaus Rajewsky +10 more
TL;DR: PicTar, a computational method for identifying common targets of micro RNAs, is presented and widespread coordinate control executed by microRNAs is suggested, thus providing evidence for coordinate microRNA control in mammals.
Journal ArticleDOI
B lymphocytes secrete antigen-presenting vesicles.
Graça Raposo,Hans W. Nijman,Willem Stoorvogel,R Liejendekker,Clifford V. Harding,Cornelis J. M. Melief,Hans J. Geuze +6 more
TL;DR: It is demonstrated by immunoelectron microscopy that the limiting membrane of MIICs can fuse directly with the plasma membrane, resulting in release from the cells of internal MHC class II-containing vesicles, suggesting a role for exosomes in antigen presentation in vivo.
Journal ArticleDOI
Identification and proteomic profiling of exosomes in human urine
TL;DR: The results indicate that exosome isolation may provide an efficient first step in biomarker discovery in urine and identify numerous protein components of MVBs and of the endosomal pathway in general.
Journal ArticleDOI
Fate of the transferrin receptor during maturation of sheep reticulocytes in vitro: Selective externalization of the receptor
Bin-Tao Pan,Rose M. Johnstone +1 more
TL;DR: The fate of the transferrin receptor during in vitro maturation of sheep reticulocytes has been followed using FITC- and 125I-labeled anti-transferrin-receptor antibodies and it can be shown that at 0 degree C or in phosphate-buffered saline the rate of vesicle release is less than that at 37 degrees C in culture medium.
Journal ArticleDOI
Embryonic stem cell-derived microvesicles reprogram hematopoietic progenitors: evidence for horizontal transfer of mRNA and protein delivery.
Janina Ratajczak,Katarzyna Miekus,Magdalena Kucia,Jin Zhang,Ryan Reca,Petr Dvorak,Mariusz Z. Ratajczak +6 more
TL;DR: ES-MV isolated from murine ES cells in serum-free cultures significantly enhanced survival and improved expansion of murine HPC, and upregulated the expression of early pluripotent and early hematopoietic stem cells in these cells.
Related Papers (5)
Extracellular vesicles: exosomes, microvesicles, and friends.
Circulating microRNAs as stable blood-based markers for cancer detection
Patrick S. Mitchell,Rachael K. Parkin,Evan M. Kroh,Brian R. Fritz,Brian R. Fritz,Stacia K. Wyman,Era L. Pogosova-Agadjanyan,Amelia Peterson,Jennifer Noteboom,Kathy O'Briant,April Allen,Daniel W. Lin,Daniel W. Lin,Daniel W. Lin,Nicole Urban,Charles W. Drescher,Beatrice S. Knudsen,Derek L. Stirewalt,Robert Gentleman,Robert L. Vessella,Robert L. Vessella,Peter S. Nelson,Daniel Martin,Daniel Martin,Muneesh Tewari +24 more