Journal ArticleDOI
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
TLDR
It is shown that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location, and it is proposed that this RNA is called “exosomal shuttle RNA” (esRNA).Abstract:
Exosomes are vesicles of endocytic origin released by many cells. These vesicles can mediate communication between cells, facilitating processes such as antigen presentation. Here, we show that exosomes from a mouse and a human mast cell line (MC/9 and HMC-1, respectively), as well as primary bone marrow-derived mouse mast cells, contain RNA. Microarray assessments revealed the presence of mRNA from approximately 1300 genes, many of which are not present in the cytoplasm of the donor cell. In vitro translation proved that the exosome mRNAs were functional. Quality control RNA analysis of total RNA derived from exosomes also revealed presence of small RNAs, including microRNAs. The RNA from mast cell exosomes is transferable to other mouse and human mast cells. After transfer of mouse exosomal RNA to human mast cells, new mouse proteins were found in the recipient cells, indicating that transferred exosomal mRNA can be translated after entering another cell. In summary, we show that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location. We propose that this RNA is called "exosomal shuttle RNA" (esRNA).read more
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Journal ArticleDOI
Broad role for YBX1 in defining the small noncoding RNA composition of exosomes.
Matthew J Shurtleff,Jun Yao,Yidan Qin,Ryan M. Nottingham,Morayma M. Temoche-Diaz,Randy Schekman,Alan M. Lambowitz +6 more
TL;DR: It is found that EV-associated transcripts are dominated by full-length, mature transfer RNAs and other small noncoding RNAs encapsulated within vesicles, suggesting that EVs and exosomes could play a role in the purging and intercellular transfer of excess free RNAs, including full- lengths tRNAs andother small ncRNAs.
Journal ArticleDOI
Extracellular Vesicles: Exosomes and Microvesicles, Integrators of Homeostasis.
TL;DR: Extracellular vesicles, cell-derived membrane structures, are secreted after fusion of endosomes with the plasma membrane (exosomes) or shed from the plasma membranes (microvesicles).
Journal ArticleDOI
Exosomes serve as tumour markers for personalized diagnostics owing to their important role in cancer metastasis
TL;DR: The roles of biomarkers, which are validated by clinical samples, are summarized in this article to show the major contribution of tumour-associated exosomes to different steps of cancer metastasis.
Journal ArticleDOI
The emerging role of exosomes in mental disorders.
TL;DR: The roles of exosomes in the brain are discussed, novel findings are related to current insights into mental disorders, and novel findings from exosome studies derived from the brain may provide a mechanistic view of the disease phenotype.
Journal ArticleDOI
Tumor-derived exosomes modulate PD-L1 expression in monocytes.
Franziska Haderk,Ralph Schulz,Murat Iskar,Laura Llao Cid,Thomas Worst,Karolin V. Willmund,Angela Schulz,Uwe Warnken,Jana Seiler,Axel Benner,Michelle Nessling,Thorsten Zenz,Maria Göbel,Jan Dürig,Sven Diederichs,Jérôme Paggetti,Etienne Moussay,Stephan Stilgenbauer,Marc Zapatka,Peter Lichter,Martina Seiffert +20 more
TL;DR: Exosome-mediated transfer of noncoding RNAs to monocytes contributes to cancer-related inflammation and concurrent immune escape via PD-L1 expression, and exosomal hY4 as a driver of TLR7 signaling is identified.
References
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Journal ArticleDOI
Identification and proteomic profiling of exosomes in human urine
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Journal ArticleDOI
Fate of the transferrin receptor during maturation of sheep reticulocytes in vitro: Selective externalization of the receptor
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Journal ArticleDOI
Embryonic stem cell-derived microvesicles reprogram hematopoietic progenitors: evidence for horizontal transfer of mRNA and protein delivery.
Janina Ratajczak,Katarzyna Miekus,Magdalena Kucia,Jin Zhang,Ryan Reca,Petr Dvorak,Mariusz Z. Ratajczak +6 more
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