Journal ArticleDOI
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
TLDR
It is shown that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location, and it is proposed that this RNA is called “exosomal shuttle RNA” (esRNA).Abstract:
Exosomes are vesicles of endocytic origin released by many cells. These vesicles can mediate communication between cells, facilitating processes such as antigen presentation. Here, we show that exosomes from a mouse and a human mast cell line (MC/9 and HMC-1, respectively), as well as primary bone marrow-derived mouse mast cells, contain RNA. Microarray assessments revealed the presence of mRNA from approximately 1300 genes, many of which are not present in the cytoplasm of the donor cell. In vitro translation proved that the exosome mRNAs were functional. Quality control RNA analysis of total RNA derived from exosomes also revealed presence of small RNAs, including microRNAs. The RNA from mast cell exosomes is transferable to other mouse and human mast cells. After transfer of mouse exosomal RNA to human mast cells, new mouse proteins were found in the recipient cells, indicating that transferred exosomal mRNA can be translated after entering another cell. In summary, we show that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location. We propose that this RNA is called "exosomal shuttle RNA" (esRNA).read more
Citations
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Extracellular vesicles - biomarkers and effectors of the cellular interactome in cancer.
TL;DR: Embracing the notion that human cancers are defined not only by processes occurring within cancer cells, but also between them, and amidst the altered tumor and systemic microenvironment may open new diagnostic and therapeutic opportunities.
Journal ArticleDOI
Cell based therapies for ischemic stroke: from basic science to bedside.
Xinfeng Liu,Ruidong Ye,Tao Yan,Tao Yan,Shan Ping Yu,Ling Wei,Gelin Xu,Xinying Fan,Yongjun Jiang,R. Anne Stetler,George Liu,Jieli Chen +11 more
TL;DR: This review attempts to provide a comprehensive synopsis of preclinical evidence and clinical experience of various donor cell types, their restorative mechanisms, delivery routes, imaging strategies, future prospects and challenges for translating cell therapies as a neurorestorative regimen in clinical applications.
Journal ArticleDOI
MicroRNAs: molecular features and role in cancer.
TL;DR: The involvement of miRNAs in several oncogenic pathways such as angiogenesis and apoptosis, and in the inter-cellular dialog mediated by miRNA-loaded exosomes as well as the development of new therapeutical strategies based on miRNas will be discussed.
Journal ArticleDOI
Circulating miR-375 as a biomarker of β-cell death and diabetes in mice.
TL;DR: Data suggest that circulating miR-375 can be used as a marker of β-cell death and potential predictor of diabetes.
Journal ArticleDOI
Cancer-derived exosomes loaded with ultrathin palladium nanosheets for targeted bioorthogonal catalysis
María Sancho-Albero,Belén Rubio-Ruiz,Ana M. Pérez-López,Victor Sebastian,Pilar Martin-Duque,Manuel Arruebo,Jesus Santamaria,Asier Unciti-Broceta +7 more
TL;DR: The synthesis of catalytically active Pd nanostructures in exosomes that selectively target cancer cells for drug activation using biorthogonal chemistry is reported.
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Journal ArticleDOI
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Journal ArticleDOI
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