Journal ArticleDOI
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
TLDR
It is shown that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location, and it is proposed that this RNA is called “exosomal shuttle RNA” (esRNA).Abstract:
Exosomes are vesicles of endocytic origin released by many cells. These vesicles can mediate communication between cells, facilitating processes such as antigen presentation. Here, we show that exosomes from a mouse and a human mast cell line (MC/9 and HMC-1, respectively), as well as primary bone marrow-derived mouse mast cells, contain RNA. Microarray assessments revealed the presence of mRNA from approximately 1300 genes, many of which are not present in the cytoplasm of the donor cell. In vitro translation proved that the exosome mRNAs were functional. Quality control RNA analysis of total RNA derived from exosomes also revealed presence of small RNAs, including microRNAs. The RNA from mast cell exosomes is transferable to other mouse and human mast cells. After transfer of mouse exosomal RNA to human mast cells, new mouse proteins were found in the recipient cells, indicating that transferred exosomal mRNA can be translated after entering another cell. In summary, we show that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location. We propose that this RNA is called "exosomal shuttle RNA" (esRNA).read more
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Gastric cancer exosomes trigger differentiation of umbilical cord derived mesenchymal stem cells to carcinoma-associated fibroblasts through TGF-β/Smad pathway.
Jianmei Gu,Hui Qian,Li Shen,Xu Zhang,Wei Zhu,Ling Huang,Yongmin Yan,Fei Mao,Chonghui Zhao,Yunyan Shi,Wenrong Xu +10 more
TL;DR: The results suggest that gastric cancer cells triggered the differentiation of hucMSCs to CAFs by exosomes-mediated TGF-β transfer and T GF-β/Smad pathway activation, which may represent a novel mechanism for MSCs toCAFs transition in cancer.
Journal ArticleDOI
Suppression of autophagy by extracellular vesicles promotes myofibroblast differentiation in COPD pathogenesis
Yu Fujita,Jun Araya,Saburo Ito,Kenji Kobayashi,Nobuyoshi Kosaka,Yusuke Yoshioka,Tsukasa Kadota,Hiromichi Hara,Kazuyoshi Kuwano,Takahiro Ochiya +9 more
TL;DR: The findings demonstrate that CSE triggers the modification of EV components and identify bronchial epithelial cell-derived miR-210 as a paracrine autophagy mediator of myofibroblast differentiation that has potential as a therapeutic target for COPD.
Journal ArticleDOI
Urine markers for detection and surveillance of non-muscle-invasive bladder cancer.
Derya Tilki,Maximilian Burger,Guido Dalbagni,H. Barton Grossman,Oliver W. Hakenberg,Juan Palou,Oliver Reich,Morgan Rouprêt,Shahrokh F. Shariat,Alexandre R. Zlotta +9 more
TL;DR: Urine markers have been studied extensively to help diagnose bladder cancer and thereby decrease the need for cystoscopy, but no marker is available at present that can sufficiently warrant this and large prospective and standardized studies are needed.
Journal ArticleDOI
Biological Activities of Extracellular Vesicles and Their Cargos from Bovine and Human Milk in Humans and Implications for Infants
Janos Zempleni,Ana Aguilar-Lozano,Mahrou Sadri,Sonal Sukreet,Sonia Manca,Di Wu,Fang Zhou,Ezra Mutai +7 more
TL;DR: Advances in research concerning milk exosomes and their cargos in human nutrition include higher Mental Developmental Index, Psychomotor Development Index, and Preschool Language Scale-3 scores in breastfed infants than in those fed various formulas.
Journal ArticleDOI
Mesenchymal stem cell therapy ameliorates diabetic nephropathy via the paracrine effect of renal trophic factors including exosomes.
Kanna Nagaishi,Yuka Mizue,Takako Chikenji,Miho Otani,Masako Nakano,Naoto Konari,Mineko Fujimiya +6 more
TL;DR: MSC therapy is a promising tool to prevent diabetic nephropathy via the paracrine effect of renal trophic factors including exosomes due to its multifactorial action.
References
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Journal ArticleDOI
Identification and proteomic profiling of exosomes in human urine
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Journal ArticleDOI
Fate of the transferrin receptor during maturation of sheep reticulocytes in vitro: Selective externalization of the receptor
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Journal ArticleDOI
Embryonic stem cell-derived microvesicles reprogram hematopoietic progenitors: evidence for horizontal transfer of mRNA and protein delivery.
Janina Ratajczak,Katarzyna Miekus,Magdalena Kucia,Jin Zhang,Ryan Reca,Petr Dvorak,Mariusz Z. Ratajczak +6 more
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