Journal ArticleDOI
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
TLDR
It is shown that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location, and it is proposed that this RNA is called “exosomal shuttle RNA” (esRNA).Abstract:
Exosomes are vesicles of endocytic origin released by many cells. These vesicles can mediate communication between cells, facilitating processes such as antigen presentation. Here, we show that exosomes from a mouse and a human mast cell line (MC/9 and HMC-1, respectively), as well as primary bone marrow-derived mouse mast cells, contain RNA. Microarray assessments revealed the presence of mRNA from approximately 1300 genes, many of which are not present in the cytoplasm of the donor cell. In vitro translation proved that the exosome mRNAs were functional. Quality control RNA analysis of total RNA derived from exosomes also revealed presence of small RNAs, including microRNAs. The RNA from mast cell exosomes is transferable to other mouse and human mast cells. After transfer of mouse exosomal RNA to human mast cells, new mouse proteins were found in the recipient cells, indicating that transferred exosomal mRNA can be translated after entering another cell. In summary, we show that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location. We propose that this RNA is called "exosomal shuttle RNA" (esRNA).read more
Citations
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Journal ArticleDOI
Cell to Cell Signalling via Exosomes Through esRNA
Jan Lötvall,Hadi Valadi +1 more
TL;DR: It is proposed that several types of exosomes may exist, and that an additional function ofExosomes is to communicate to neighboring cells through delivery of RNA-signals.
Journal ArticleDOI
Surface functionalization of exosomes using click chemistry
Tyson Smyth,Krastina Petrova,Nicole M. Payton,Indushekhar Persaud,Jasmina S. Redzic,Michael W. Graner,Peter Smith-Jones,Thomas J. Anchordoquy +7 more
TL;DR: Conjugation had no effect on the size of exosomes, nor was there any change in the extent of exOSome adherence/internalization with recipient cells, suggesting the reaction conditions were mild on exosome structure and function.
Journal ArticleDOI
Nanoparticle tracking analysis monitors microvesicle and exosome secretion from immune cells
Chin Y. Soo,Yaqiong Song,Ying Zheng,Elaine C. Campbell,Andrew Riches,Frank J. Gunn-Moore,Simon J. Powis +6 more
TL;DR: Treatment of peripheral blood monocyte‐derived dendritic cells with bacterial lipopolysaccharide displayed an increase in secreted microvesicles, demonstrating that nanoparticle tracking analysis can be effectively applied to monitor microvesicle release from cells of the immune system.
Journal ArticleDOI
Exosome nanotechnology: an emerging paradigm shift in drug delivery: exploitation of exosome nanovesicles for systemic in vivo delivery of RNAi heralds new horizons for drug delivery across biological barriers.
Samira Lakhal,Matthew J.A. Wood +1 more
TL;DR: The demonstration that dendritic cell (DC)‐derived exosomes can be exploited for targeted RNAi delivery to the brain after systemic injection provides the first proof‐of‐concept for the potential of these naturally occurring vesicles as vehicles of drug delivery.
Journal ArticleDOI
The microRNA regulatory landscape of MSC-derived exosomes: a systems view.
Scott W. Ferguson,Jinli Wang,Christine J. Lee,Maixian Liu,Sriram Neelamegham,John M. Canty,Juliane Nguyen +6 more
TL;DR: The data presented here help define the miRNA landscape of MSC exosomes, establish their biological functions through network analyses at a system level, and provide a platform for modulating the overall phenotypic effects of exosome.
References
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Journal ArticleDOI
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Journal ArticleDOI
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Journal ArticleDOI
Embryonic stem cell-derived microvesicles reprogram hematopoietic progenitors: evidence for horizontal transfer of mRNA and protein delivery.
Janina Ratajczak,Katarzyna Miekus,Magdalena Kucia,Jin Zhang,Ryan Reca,Petr Dvorak,Mariusz Z. Ratajczak +6 more
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