Journal ArticleDOI
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
TLDR
It is shown that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location, and it is proposed that this RNA is called “exosomal shuttle RNA” (esRNA).Abstract:
Exosomes are vesicles of endocytic origin released by many cells. These vesicles can mediate communication between cells, facilitating processes such as antigen presentation. Here, we show that exosomes from a mouse and a human mast cell line (MC/9 and HMC-1, respectively), as well as primary bone marrow-derived mouse mast cells, contain RNA. Microarray assessments revealed the presence of mRNA from approximately 1300 genes, many of which are not present in the cytoplasm of the donor cell. In vitro translation proved that the exosome mRNAs were functional. Quality control RNA analysis of total RNA derived from exosomes also revealed presence of small RNAs, including microRNAs. The RNA from mast cell exosomes is transferable to other mouse and human mast cells. After transfer of mouse exosomal RNA to human mast cells, new mouse proteins were found in the recipient cells, indicating that transferred exosomal mRNA can be translated after entering another cell. In summary, we show that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location. We propose that this RNA is called "exosomal shuttle RNA" (esRNA).read more
Citations
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Trafficking and function of the tetraspanin CD63.
Maaike S. Pols,Judith Klumperman +1 more
TL;DR: The latest insights in CD63 trafficking and its emerging function as a transport regulator of its interaction partners are discussed and the involvement of CD63 in cancer will be discussed.
Journal ArticleDOI
Sorting it out: Regulation of exosome loading
Carolina Villarroya-Beltri,Francesc Baixauli,Cristina Gutiérrez-Vázquez,Francisco Sánchez-Madrid,María Mittelbrunn +4 more
TL;DR: How the cellular context affects the composition of exosomes, and thus the outcome of the communication between the exosome-producer and recipient cells, is discussed, with particular focus on the Communication between tumor cells and with cells of the tumor microenvironment.
Journal ArticleDOI
Exosome-delivered microRNAs modulate the inflammatory response to endotoxin
Margaret Alexander,Ruozhen Hu,Marah C. Runtsch,Dominique Kagele,Timothy L. Mosbruger,Tanya Tolmachova,Miguel C. Seabra,June L. Round,Diane M. Ward,Ryan M. O'Connell +9 more
TL;DR: Findings provide strong evidence that endogenous microRNAs undergo a functional transfer between immune cells and constitute a mechanism of regulating the inflammatory response.
Journal ArticleDOI
Extracellular vesicles from human cardiac progenitor cells inhibit cardiomyocyte apoptosis and improve cardiac function after myocardial infarction.
Lucio Barile,Vincenzo Lionetti,Elisabetta Cervio,Marco Matteucci,Mihaela Gherghiceanu,Laurentiu M. Popescu,T. Torre,Francesco Siclari,Tiziano Moccetti,Giuseppe Vassalli +9 more
TL;DR: In this paper, the extracellular vesicles (EVs) secreted by human cardiac progenitor cells (CPCs) are shown to act as paracrine signalling mediators.
Journal ArticleDOI
Circulating miRNAs are correlated with tumor progression in prostate cancer.
Jan C. Brase,Marc Johannes,Thorsten Schlomm,Maria Fälth,Alexander Haese,Thomas Steuber,Tim Beissbarth,Ruprecht Kuner,Holger Sültmann +8 more
TL;DR: Observations suggest that mi RNA‐375 and miRNA‐141 expression is enhanced in prostate cancer specimens and their release into the blood is further associated with advanced cancer disease.
References
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Journal ArticleDOI
Identification and proteomic profiling of exosomes in human urine
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Journal ArticleDOI
Fate of the transferrin receptor during maturation of sheep reticulocytes in vitro: Selective externalization of the receptor
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TL;DR: The fate of the transferrin receptor during in vitro maturation of sheep reticulocytes has been followed using FITC- and 125I-labeled anti-transferrin-receptor antibodies and it can be shown that at 0 degree C or in phosphate-buffered saline the rate of vesicle release is less than that at 37 degrees C in culture medium.
Journal ArticleDOI
Embryonic stem cell-derived microvesicles reprogram hematopoietic progenitors: evidence for horizontal transfer of mRNA and protein delivery.
Janina Ratajczak,Katarzyna Miekus,Magdalena Kucia,Jin Zhang,Ryan Reca,Petr Dvorak,Mariusz Z. Ratajczak +6 more
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