Journal ArticleDOI
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
TLDR
It is shown that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location, and it is proposed that this RNA is called “exosomal shuttle RNA” (esRNA).Abstract:
Exosomes are vesicles of endocytic origin released by many cells. These vesicles can mediate communication between cells, facilitating processes such as antigen presentation. Here, we show that exosomes from a mouse and a human mast cell line (MC/9 and HMC-1, respectively), as well as primary bone marrow-derived mouse mast cells, contain RNA. Microarray assessments revealed the presence of mRNA from approximately 1300 genes, many of which are not present in the cytoplasm of the donor cell. In vitro translation proved that the exosome mRNAs were functional. Quality control RNA analysis of total RNA derived from exosomes also revealed presence of small RNAs, including microRNAs. The RNA from mast cell exosomes is transferable to other mouse and human mast cells. After transfer of mouse exosomal RNA to human mast cells, new mouse proteins were found in the recipient cells, indicating that transferred exosomal mRNA can be translated after entering another cell. In summary, we show that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location. We propose that this RNA is called "exosomal shuttle RNA" (esRNA).read more
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Gliomas and the vascular fragility of the blood brain barrier
Luiz Gustavo Dubois,Loraine Campanati,Cassia Righy,Isabella D’Andrea-Meira,Tania Cristina Leite de Sampaio e Spohr,Isabel Porto-Carreiro,Cláudia Pereira,Joana Balça-Silva,Suzana Assad Kahn,Marcos F. DosSantos,Marcela de Almeida Rabello Oliveira,Adriana Ximenes-da-Silva,Maria do Carmo Lopes,Eduardo C. Faveret,Emerson Leandro Gasparetto,Vivaldo Moura-Neto +15 more
TL;DR: Astrocytes, members of the glial family, interact through the exchange of soluble factors or by directly contacting neurons and other brain cells, such as microglia and endothelial cells, and act as additional elements of the Blood Brain Barrier (BBB).
Journal ArticleDOI
MicroRNAs as potential biomarkers in human solid tumors
TL;DR: The progress of analysis of microRNAs is reviewed as a potential approach for diagnosis and prognosis of solid cancer and obstacles in developing miRNAs as circulating biomarkers are discussed.
Journal ArticleDOI
Lactation-Related MicroRNA Expression Profiles of Porcine Breast Milk Exosomes
Yiren Gu,Mingzhou Li,Mingzhou Li,Tao Wang,Liang Yan,Zhijun Zhong,Xiaoyan Wang,Qi Zhou,Lei Chen,Qiulei Lang,Zhiping He,Xiaohui Chen,Gong Jianjun,Xiaolian Gao,Xuewei Li,Xuebin Lv +15 more
TL;DR: It is found that immune-related miRNAs are present and enriched in breast milk exosomes and are generally resistant to relatively harsh conditions.
Journal ArticleDOI
Different gDNA content in the subpopulations of prostate cancer extracellular vesicles: apoptotic bodies, microvesicles, and exosomes.
Elisa Lázaro-Ibáñez,Andres Sanz-Garcia,Tapio Visakorpi,Carmen Escobedo-Lucea,Pia Siljander,Angel Ayuso-Sacido,Marjo Yliperttula +6 more
TL;DR: The aim of this study was to analyze whether different prostate cancer cell‐derived EV subpopulations (apoptotic bodies, microvesicles, and exosomes) carry different gDNA fragments.
Journal ArticleDOI
Exosomes secreted from human colorectal cancer cell lines contain mRNAs, microRNAs and natural antisense RNAs, that can transfer into the human hepatoma HepG2 and lung cancer A549 cell lines
TL;DR: It is revealed that exosomes derived from the CRC cell lines contain mRNAs, microRNAs and natural antisense RNAs, and can be delivered into HepG2 and A549 cells, which indicates thatExosomal RNAs can shuttle between cells, and may be involved in the regulation of gene expression in recipient cells.
References
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Journal ArticleDOI
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Journal ArticleDOI
Identification and proteomic profiling of exosomes in human urine
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Journal ArticleDOI
Fate of the transferrin receptor during maturation of sheep reticulocytes in vitro: Selective externalization of the receptor
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TL;DR: The fate of the transferrin receptor during in vitro maturation of sheep reticulocytes has been followed using FITC- and 125I-labeled anti-transferrin-receptor antibodies and it can be shown that at 0 degree C or in phosphate-buffered saline the rate of vesicle release is less than that at 37 degrees C in culture medium.
Journal ArticleDOI
Embryonic stem cell-derived microvesicles reprogram hematopoietic progenitors: evidence for horizontal transfer of mRNA and protein delivery.
Janina Ratajczak,Katarzyna Miekus,Magdalena Kucia,Jin Zhang,Ryan Reca,Petr Dvorak,Mariusz Z. Ratajczak +6 more
TL;DR: ES-MV isolated from murine ES cells in serum-free cultures significantly enhanced survival and improved expansion of murine HPC, and upregulated the expression of early pluripotent and early hematopoietic stem cells in these cells.
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