Journal ArticleDOI
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
TLDR
It is shown that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location, and it is proposed that this RNA is called “exosomal shuttle RNA” (esRNA).Abstract:
Exosomes are vesicles of endocytic origin released by many cells. These vesicles can mediate communication between cells, facilitating processes such as antigen presentation. Here, we show that exosomes from a mouse and a human mast cell line (MC/9 and HMC-1, respectively), as well as primary bone marrow-derived mouse mast cells, contain RNA. Microarray assessments revealed the presence of mRNA from approximately 1300 genes, many of which are not present in the cytoplasm of the donor cell. In vitro translation proved that the exosome mRNAs were functional. Quality control RNA analysis of total RNA derived from exosomes also revealed presence of small RNAs, including microRNAs. The RNA from mast cell exosomes is transferable to other mouse and human mast cells. After transfer of mouse exosomal RNA to human mast cells, new mouse proteins were found in the recipient cells, indicating that transferred exosomal mRNA can be translated after entering another cell. In summary, we show that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location. We propose that this RNA is called "exosomal shuttle RNA" (esRNA).read more
Citations
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Journal ArticleDOI
RNA-based diagnostic and therapeutic strategies for cardiovascular disease
TL;DR: This Review highlights viral-based delivery tools that have been widely used to evaluate the therapeutic utility of both coding and non-coding RNAs in the context of cardiovascular disease and discusses the potential of using oligonucleotide-based molecular products such as modified RNA, small interfering RNA and RNA mimics/inhibitors for the treatment of cardiovascular diseases
Journal ArticleDOI
MicroRNAs in inflammation and immune responses.
Jorge R. Contreras,Dinesh S. Rao +1 more
TL;DR: Better understood mechanisms as well as some emerging concepts of miRNA function are reviewed, which will likely involve defining the function of specific miRNAs in specific immune cell lineages and to utilize them in the design of therapeutic strategies for diseases involving the immune system.
Journal ArticleDOI
New evidence that a large proportion of human blood plasma cell-free DNA is localized in exosomes.
TL;DR: Evidence is provided that a large proportion of plasma cfDNA is localized in exosomes, suggesting active release of cfDNA from cells as a source ofcfDNA in plasma.
Journal ArticleDOI
Circulating miRNAs as sensitive and specific biomarkers for the diagnosis and monitoring of human diseases: promises and challenges.
V. De Guire,Richard Robitaille,Nicolas Tetreault,Renée Guérin,Catherine Ménard,N. Bambace,Przemyslaw Sapieha +6 more
TL;DR: The practicality of miRNAs in the screening, diagnosis and prognosis of a range of pathologies and the design of future miRNA-based detection strategies for use in clinical biochemistry laboratory settings are discussed.
Journal ArticleDOI
Native and bioengineered extracellular vesicles for cardiovascular therapeutics
Ricardo Cerqueira de Abreu,Ricardo Cerqueira de Abreu,Hugo Fernandes,Paula A. da Costa Martins,Susmita Sahoo,Costanza Emanueli,Lino Ferreira +6 more
TL;DR: Vesicles secreted from stem or progenitor cells and from differentiated somatic cells have regenerative properties in the context of myocardial infarction, ischaemic limb disease and stroke.
References
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Journal ArticleDOI
Identification and proteomic profiling of exosomes in human urine
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Journal ArticleDOI
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Journal ArticleDOI
Embryonic stem cell-derived microvesicles reprogram hematopoietic progenitors: evidence for horizontal transfer of mRNA and protein delivery.
Janina Ratajczak,Katarzyna Miekus,Magdalena Kucia,Jin Zhang,Ryan Reca,Petr Dvorak,Mariusz Z. Ratajczak +6 more
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