Journal ArticleDOI
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
TLDR
It is shown that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location, and it is proposed that this RNA is called “exosomal shuttle RNA” (esRNA).Abstract:
Exosomes are vesicles of endocytic origin released by many cells. These vesicles can mediate communication between cells, facilitating processes such as antigen presentation. Here, we show that exosomes from a mouse and a human mast cell line (MC/9 and HMC-1, respectively), as well as primary bone marrow-derived mouse mast cells, contain RNA. Microarray assessments revealed the presence of mRNA from approximately 1300 genes, many of which are not present in the cytoplasm of the donor cell. In vitro translation proved that the exosome mRNAs were functional. Quality control RNA analysis of total RNA derived from exosomes also revealed presence of small RNAs, including microRNAs. The RNA from mast cell exosomes is transferable to other mouse and human mast cells. After transfer of mouse exosomal RNA to human mast cells, new mouse proteins were found in the recipient cells, indicating that transferred exosomal mRNA can be translated after entering another cell. In summary, we show that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location. We propose that this RNA is called "exosomal shuttle RNA" (esRNA).read more
Citations
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Transplanted Mesenchymal Stem Cells Reduce Autophagic Flux in Infarcted Hearts via the Exosomal Transfer of miR-125b.
Changchen Xiao,Kan Wang,Yinchuan Xu,Hengxun Hu,Na Zhang,Yingchao Wang,Zhiwei Zhong,Jing Zhao,Qingju Li,Dan Zhu,Changle Ke,Shuhan Zhong,Xianpeng Wu,Hong Yu,Wei Zhu,Jinghai Chen,Jianyi Zhang,Jian-an Wang,Xinyang Hu +18 more
TL;DR: The beneficial effects offered by MSC transplantation after myocardial infarction are at least partially because of improved autophagic flux through excreted exosome containing mainly miR-125b-5p.
Journal ArticleDOI
Identification of non-invasive miRNAs biomarkers for prostate cancer by deep sequencing analysis of urinary exosomes.
Marta Rodríguez,Marta Rodríguez,Cristina Bajo-Santos,Nina Pettersen Hessvik,Nina Pettersen Hessvik,Susanne Lorenz,Bastian Fromm,Viktor Berge,Kirsten Sandvig,Kirsten Sandvig,Aija Linē,Alicia Llorente,Alicia Llorente +12 more
TL;DR: Results suggest that specific microRNAs in urinary exosomes might serve as non-invasive biomarkers for prostate cancer and miR-196a-5p and mi-501-3p are promising biomarkers that need to be further studied in large patient cohorts.
Journal ArticleDOI
Molecular characterization of exosomes and their microRNA cargo in human follicular fluid: bioinformatic analysis reveals that exosomal microRNAs control pathways involved in follicular maturation.
Manuela Santonocito,Marilena Vento,Maria Rosa Guglielmino,Rosalia Battaglia,Jessica Wahlgren,Marco Ragusa,Davide Barbagallo,Placido Borzì,Simona Rizzari,Marco Maugeri,Paolo Scollo,Carla Tatone,Hadi Valadi,Michele Purrello,Cinzia Di Pietro +14 more
TL;DR: This study identified a series of exosomal microRNAs that are highly represented in human FF and are involved in follicular maturation and could represent noninvasive biomarkers of oocyte quality in assisted reproductive technology.
Journal ArticleDOI
MicroRNAs and bacterial infection
Cathy Staedel,Fabien Darfeuille +1 more
TL;DR: This review presents the current state of knowledge about the role of miRNAs in the response to both bacterial pathogens and commensal bacteria in human cells or animal experimental models.
Journal ArticleDOI
Serum and urinary cell-free MiR-146a and MiR-155 in patients with systemic lupus erythematosus.
Gang Wang,Lai-Shan Tam,Edmund K. Li,Bonnie Ching-Ha Kwan,Kai-Ming Chow,Cathy Choi-Wan Luk,Philip Kam-Tao Li,Cheuk-Chun Szeto +7 more
TL;DR: The results suggested that serummiR-146a and miR-155 participate in the pathophysiology of SLE and might be used as biomarkers of S LE.
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