Journal ArticleDOI
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
TLDR
It is shown that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location, and it is proposed that this RNA is called “exosomal shuttle RNA” (esRNA).Abstract:
Exosomes are vesicles of endocytic origin released by many cells. These vesicles can mediate communication between cells, facilitating processes such as antigen presentation. Here, we show that exosomes from a mouse and a human mast cell line (MC/9 and HMC-1, respectively), as well as primary bone marrow-derived mouse mast cells, contain RNA. Microarray assessments revealed the presence of mRNA from approximately 1300 genes, many of which are not present in the cytoplasm of the donor cell. In vitro translation proved that the exosome mRNAs were functional. Quality control RNA analysis of total RNA derived from exosomes also revealed presence of small RNAs, including microRNAs. The RNA from mast cell exosomes is transferable to other mouse and human mast cells. After transfer of mouse exosomal RNA to human mast cells, new mouse proteins were found in the recipient cells, indicating that transferred exosomal mRNA can be translated after entering another cell. In summary, we show that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location. We propose that this RNA is called "exosomal shuttle RNA" (esRNA).read more
Citations
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Journal ArticleDOI
Exosomal transfer of stroma-derived miR21 confers paclitaxel resistance in ovarian cancer cells through targeting APAF1.
Chi Lam Au Yeung,Ngai Na Co,Tetsushi Tsuruga,Tsz-Lun Yeung,Suet Ying Kwan,Cecilia S. Leung,Yong Li,Edward S. Lu,Kenny Kwan,Kwong Kwok Wong,Rosemarie Schmandt,Karen H. Lu,Samuel C. Mok +12 more
TL;DR: The data suggest that the malignant phenotype of metastatic ovarian cancer cells can be altered by miR21 delivered by exosomes derived from neighbouring stromal cells in the omental tumour microenvironment, and that inhibiting the transfer of stronal-derived miR 21 is an alternative modality in the treatment of metastatics and recurrent ovarian cancer.
Journal ArticleDOI
Human bone marrow- and adipose-mesenchymal stem cells secrete exosomes enriched in distinctive miRNA and tRNA species.
Serena Rubina Baglìo,Koos Rooijers,Danijela Koppers-Lalic,Frederik J. Verweij,M Pérez Lanzón,Nicoletta Zini,B.A. Naaijkens,Francesca Perut,Hans W.M. Niessen,Nicola Baldini,D. Michiel Pegtel +10 more
TL;DR: It is demonstrated that primary MSCs release small RNAs via exosomes, which are increasingly implicated in intercellular communications, and may help to understand how M SCs impact neighboring or distant cells with possible consequences for their therapeutic usage.
Journal ArticleDOI
Molecular lipidomics of exosomes released by PC-3 prostate cancer cells.
Alicia Llorente,Tore Skotland,Tore Skotland,Tuulia Sylvänne,Dimple Kauhanen,Tomasz Róg,Adam Orłowski,Ilpo Vattulainen,Ilpo Vattulainen,Kim Ekroos,Kirsten Sandvig,Kirsten Sandvig +11 more
TL;DR: The detailed lipid composition provided in this study may be useful to understand the mechanism of exosome formation, release and function, and several of the lipids enriched in exosomes could potentially be used as cancer biomarkers.
Journal ArticleDOI
Let-7 MicroRNA Family Is Selectively Secreted into the Extracellular Environment via Exosomes in a Metastatic Gastric Cancer Cell Line
Keiichi Ohshima,Kanako Inoue,Akemi Fujiwara,Keiichi Hatakeyama,Kaori Kanto,Yuko Watanabe,Koji Muramatsu,Yorikane Fukuda,Shun-ichiro Ogura,Ken Yamaguchi,Tohru Mochizuki +10 more
TL;DR: The enrichment of let-7 miRNA family in the extracellular fractions, particularly, in the exosomes from AZ-P7a cells may reflect their oncogenic characteristics including tumorigenesis and metastasis.
Journal ArticleDOI
Extracellular vesicles from blood plasma: determination of their morphology, size, phenotype and concentration.
N. Arraud,Romain Linares,Sisareuth Tan,Céline Gounou,J.-M. Pasquet,Stéphane Mornet,Alain Brisson +6 more
TL;DR: In this article, an original approach based on sedimentation on electron microscopy grids was developed for enumerating membranous extracellular vesicles (EVs), which are considered to derive from activated or apoptotic cells.
References
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Journal ArticleDOI
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Journal ArticleDOI
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