Journal ArticleDOI
Functional role of type I and type II interferons in antiviral defense.
Ulrike Müller,Ulrich Steinhoff,Luiz F. L. Reis,Silvio Hemmi,Jovan Pavlovic,Rolf M. Zinkernagel,Michel Aguet +6 more
TLDR
Comparison of mice lacking either type I or type II IFN receptors showed that, at least in response to some viruses, both IFN systems are essential for antiviral defense and are functionally nonredundant.Abstract:
Mice lacking the known subunit of the type I interferon (IFN) receptor were completely unresponsive to type I IFNs, suggesting that this receptor chain is essential for type I IFN-mediated signal transduction. These mice showed no overt anomalies but were unable to cope with viral infections, despite otherwise normal immune responses. Comparison of mice lacking either type I or type II IFN receptors showed that, at least in response to some viruses, both IFN systems are essential for antiviral defense and are functionally nonredundant.read more
Citations
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Journal ArticleDOI
Structural Characterization of SARS-CoV-2: Where We Are, and Where We Need to Be
TL;DR: Structural studies have allowed to comprehend the molecular basis underlying the role of many of the SARS-CoV-2 proteins, and to make rapid progress towards treatment and preventive therapeutics.
Journal ArticleDOI
Vesicular Stomatitis Virus as an Oncolytic Vector
TL;DR: The data and others demonstrate that VSV as well as other RNA viruses could provide a promising and exciting approach to cancer therapy and could increase a tumor's susceptibility to chemotherapeutic agents and/ or importantly, the host immune response.
Journal ArticleDOI
Noncytopathic clearance of lymphocytic choriomeningitis virus from the hepatocyte.
TL;DR: Results indicate that, like HBV, LCMV is also susceptible to intracellular inactivation by cytokine-induced antiviral mechanisms that are operative in the hepatocyte, and may have evolved to maintain the functional integrity of vital organs in the face of massive infection.
Journal ArticleDOI
Crimean-Congo hemorrhagic fever virus infection is lethal for adult type I interferon receptor-knockout mice.
Sándor Bereczky,Gunnel Lindegren,Helen Karlberg,Sara Åkerström,Jonas Klingström,Jonas Klingström,Ali Mirazimi,Ali Mirazimi +7 more
TL;DR: Using a small-animal model system, this study showed that adult mice missing the type I interferon (IFN) receptor (ifNAR(-/-)) were susceptible to CCHFV and developed an acute disease with fatal outcome, and that IFN is important for limiting virus spread and protecting against liver damage in mice.
Book ChapterDOI
Gene Targeting in Mice: a Review
Hicham Bouabe,Klaus Okkenhaug +1 more
TL;DR: An updated summary of the gene targeting technology is given and some important considerations in the design of gene-targeted mice are discussed.
References
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Journal ArticleDOI
Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family
TL;DR: This sequence allowed the determination of the hitherto unknown primary structure of rat GAPDH which is 333 aminoacids long and revealed a high degree of sequence conservation at both nucleotide and protein levels.
Journal ArticleDOI
Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes
TL;DR: A positive and negative selection procedure is described that enriches 2,000-fold for those cells that contain a targeted mutation in mouse embryo-derived stem cells.
Journal ArticleDOI
Normal development and behaviour of mice lacking the neuronal cell-surface PrP protein
Hansruedi Büeler,Marek Fischer,Yolande Lang,Yolande Lang,Horst Bluethmann,Horst Bluethmann,Hans-Peter Lipp,Stephen J. DeArmond,Stephen J. DeArmond,Stanley B. Prusiner,Stanley B. Prusiner,Michel Aguet,Charles Weissmann +12 more
TL;DR: It is now feasible to determine whether mice devoid of PrPc can propagate prions and are susceptible to scrapie pathogenesis.
Journal ArticleDOI
Immune response in mice that lack the interferon-gamma receptor.
Sui Huang,Wiljan Hendriks,Alana Althage,Silvio Hemmi,Horst Bluethmann,Ryutaro Kamijo,Jan Vilcek,Rolf M. Zinkernagel,Michel Aguet +8 more
TL;DR: Mutant mice offer the possibility for the further elucidation of IFN-gamma-mediated functions by transgenic cell- or tissue-specific reconstitution of a functional receptor.
Journal ArticleDOI
Regulated expression of a gene encoding a nuclear factor, IRF-1, that specifically binds to IFN-β gene regulatory elements
Masaaki Miyamoto,Takashi Fujita,Yoko Kimura,Mitsuo Maruyama,Hisashi Harada,Yoshiaki Sudo,Takashi Miyata,Tadatsugu Taniguchi +7 more
TL;DR: It is shown here that the IRF-1 gene possesses virus-inducible promoter and is also involved in the regulation of other genes such as IFN-alpha and MHC class I genes.