SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor
Markus Hoffmann,Hannah Kleine-Weber,Simon Schroeder,Nadine Krüger,Tanja Herrler,Sandra Erichsen,Tobias S. Schiergens,Georg Herrler,Nai Huei Wu,Andreas Nitsche,Marcel A. Müller,Christian Drosten,Christian Drosten,Stefan Pöhlmann +13 more
TLDR
It is demonstrated that SARS-CoV-2 uses the SARS -CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming, and it is shown that the sera from convalescent SARS patients cross-neutralized Sars-2-S-driven entry.About:
This article is published in Cell.The article was published on 2020-04-16 and is currently open access. It has received 15362 citations till now. The article focuses on the topics: Proteases.read more
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Covid-19: Perspectives on Innate Immune Evasion.
TL;DR: Knowing the immune responses to SARS-CoV-2 and its immunoevasion approaches will improve the understanding of pathogenesis, virus clearance, and contribute toward vaccine and immunotherepeutic design and evaluation.
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A review on drug repurposing applicable to COVID-19.
TL;DR: Network-based approaches allowed to annotate some important patterns, to identify proteins that are functionally associated with COVID-19 and to discover novel drug–disease or drug–target relationships useful for new therapies.
Posted ContentDOI
Global Spread of SARS-CoV-2 Subtype with Spike Protein Mutation D614G is Shaped by Human Genomic Variations that Regulate Expression of TMPRSS2 and MX1 Genes
Chandrika Bhattacharyya,Chitrarpita Das,Arnab Ghosh,Animesh K. Singh,Souvik Mukherjee,Partha P. Majumder,Analabha Basu,Nidhan K. Biswas +7 more
TL;DR: SARS-CoV-2, particularly the 614G subtype, has spread more easily and with higher frequency to Europe and North America where the delC allele regulating expression of TMPRSS2 and MX1 host proteins is common, but not to East Asia where this allele is rare.
Journal ArticleDOI
Co-expression of SARS-CoV-2 entry genes in the superficial adult human conjunctival, limbal and corneal epithelium suggests an additional route of entry via the ocular surface.
Joseph Collin,Rachel Queen,Darin Zerti,Birthe Dorgau,Maria Georgiou,Ivo Djidrovski,Rafiqul Hussain,Jonathan Coxhead,Agatha Joseph,Paul Rooney,Steven Lisgo,Francisco C. Figueiredo,Lyle Armstrong,Majlinda Lako +13 more
TL;DR: The data indicate that the human ocular surface epithelium provides an additional entry portal for SARS-CoV-2, which may exploit inflammatory driven upregulation of ACE2 and TMPRSS2 entry factors to enhance infection.
Journal ArticleDOI
Functional interrogation of a SARS-CoV-2 host protein interactome identifies unique and shared coronavirus host factors.
H.-Heinrich Hoffmann,Francisco J. Sánchez-Rivera,William M. Schneider,Joseph M. Luna,Yadira M. Soto-Feliciano,Alison W. Ashbrook,Jérémie Le Pen,Andrew A. Leal,Inna Ricardo-Lax,Eleftherios Michailidis,Yuan Hao,Ansgar F. Stenzel,Ansgar F. Stenzel,Avery Peace,Johannes Zuber,Johannes Zuber,C. David Allis,Scott W. Lowe,Margaret R. MacDonald,John T. Poirier,Charles M. Rice +20 more
TL;DR: A focused high-coverage CRISPR-Cas9 library targeting 332 members of a recently published SARS-CoV-2 protein interactome yielded several insights, including potential virus-specific differences in Rab GTPase requirements and glycosylphosphatidylinositol (GPI) anchor biosynthesis, as well as identification of multiple pan-coronavirus factors involved in cholesterol homeostasis.
References
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