SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor
Markus Hoffmann,Hannah Kleine-Weber,Simon Schroeder,Nadine Krüger,Tanja Herrler,Sandra Erichsen,Tobias S. Schiergens,Georg Herrler,Nai Huei Wu,Andreas Nitsche,Marcel A. Müller,Christian Drosten,Christian Drosten,Stefan Pöhlmann +13 more
TLDR
It is demonstrated that SARS-CoV-2 uses the SARS -CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming, and it is shown that the sera from convalescent SARS patients cross-neutralized Sars-2-S-driven entry.About:
This article is published in Cell.The article was published on 2020-04-16 and is currently open access. It has received 15362 citations till now. The article focuses on the topics: Proteases.read more
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Monocytes and Macrophages in COVID-19.
TL;DR: In this article, the authors outline current knowledge on monocytes and macrophages in homeostasis and viral infections and summarize concepts and key findings on their role in COVID-19.
Journal ArticleDOI
Stabilizing the closed SARS-CoV-2 spike trimer.
Jarek Juraszek,Lucy Rutten,Sven Blokland,Pascale Bouchier,Richard Voorzaat,Tina Ritschel,Mark J. G. Bakkers,Ludovic Renault,Johannes P. M. Langedijk +8 more
TL;DR: In this article, the structure-based design of soluble S trimers with increased yields and stabilities, based on introduction of single point mutations and disulfide-bridges, was reported.
Journal ArticleDOI
In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.
Lei Sun,Pan Li,Xiaohui Ju,Jian Rao,Wenze Huang,Lili Ren,Shaojun Zhang,Tuanlin Xiong,Kui Xu,Xiaolin Zhou,Mingli Gong,Eric A. Miska,Eric A. Miska,Qiang Ding,Jianwei Wang,Qiangfeng Cliff Zhang +15 more
TL;DR: In this article, the structural landscape of SARS-CoV-2 RNA in infected human cells and from refolded RNAs, as well as the regulatory untranslated regions of six other coronaviruses were determined using icSHAPE.
Journal ArticleDOI
Infectability of human BrainSphere neurons suggests neurotropism of SARS-CoV-2
C. Korin Bullen,Helena T. Hogberg,Asli Bahadirli-Talbott,William R. Bishai,Thomas Hartung,Thomas Hartung,Casey Keuthan,Monika M Looney,Andrew Pekosz,J. Carolina Romero,Fenna C.M. Sillé,Peter K. Um,Lena Smirnova +12 more
TL;DR: Leveraging state-of-the-art 3D organotypic cell culture, which has been shown to allow both virus infection and (developmental) neurotoxicity but is at the same time simple enough to be transferred and used in a BSL-3 environment, it is demonstrated, for the first time, the potential critically important neurotropism of SARS-CoV-2.
Journal ArticleDOI
COVID 19: a clue from innate immunity.
Domenico Birra,Maurizio Benucci,Luigi Landolfi,Anna Merchionda,Gabriella Loi,Patrizia Amato,Gaetano Licata,Luca Quartuccio,Massimo Triggiani,Paolo Moscato +9 more
TL;DR: Innate immunity, as one of the first elements of guest interaction with different infectious agents, could play an important role in the development of the cytokine storm and be responsible for boosting more severe forms of the disease.
References
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TL;DR: The findings are consistent with person-to-person transmission of this novel coronavirus in hospital and family settings, and the reports of infected travellers in other geographical regions.
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