SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor
Markus Hoffmann,Hannah Kleine-Weber,Simon Schroeder,Nadine Krüger,Tanja Herrler,Sandra Erichsen,Tobias S. Schiergens,Georg Herrler,Nai Huei Wu,Andreas Nitsche,Marcel A. Müller,Christian Drosten,Christian Drosten,Stefan Pöhlmann +13 more
TLDR
It is demonstrated that SARS-CoV-2 uses the SARS -CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming, and it is shown that the sera from convalescent SARS patients cross-neutralized Sars-2-S-driven entry.About:
This article is published in Cell.The article was published on 2020-04-16 and is currently open access. It has received 15362 citations till now. The article focuses on the topics: Proteases.read more
Citations
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Features, Evaluation, and Treatment of Coronavirus
TL;DR: The SARS-CoV-2 virus, a novel virus belonging to the coronavirus (CoV) family, has become the major pathogens of emerging respiratory disease outbreaks and has quickly spread globally.
Journal ArticleDOI
TMPRSS2 and furin are both essential for proteolytic activation of SARS-CoV-2 in human airway cells.
Dorothea Bestle,Miriam Ruth Heindl,Hannah Limburg,Thuy Van Lam van,Oliver Pilgram,Hong M. Moulton,David A. Stein,Kornelia Hardes,Kornelia Hardes,Markus Eickmann,Olga Dolnik,Cornelius Rohde,Hans-Dieter Klenk,Wolfgang Garten,Torsten Steinmetzer,Eva Böttcher-Friebertshäuser +15 more
TL;DR: It is shown that S can be cleaved by the proprotein convertase furin at the S1/S2 site and the transmembrane serine protease 2 (TMPRSS2) at theS2′ site, and this approach has considerable therapeutic potential for treatment of COVID-19.
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Recognizing COVID-19-related myocarditis: The possible pathophysiology and proposed guideline for diagnosis and management.
Bhurint Siripanthong,Saman Nazarian,Daniele Muser,Rajat Deo,Pasquale Santangeli,Mohammed Y Khanji,Mohammed Y Khanji,Leslie T. Cooper,C. Anwar A. Chahal,C. Anwar A. Chahal,C. Anwar A. Chahal +10 more
TL;DR: Clinicians should be vigilant to provide prompt monitoring and treatments of COVID-19 patients and an endomyocardial biopsy may help identify active cardiac infection through viral genome amplification and possibly refine the treatment risks of systemic immunosuppression.
Journal ArticleDOI
Reduced neutralization of SARS-CoV-2 B.1.617 by vaccine and convalescent serum.
Chang Liu,Helen M. Ginn,Wanwisa Dejnirattisai,P Supasa,Beibei Wang,Aekkachai Tuekprakhon,R Nutalai,D. Zhou,Alexander J. Mentzer,Yuguang Zhao,Duyvesteyn Hme.,Cesar Lopez-Camacho,J Slon-Campos,Thomas S. Walter,Donal T. Skelly,S A Johnson,T G Ritter,C Mason,S A Costa Clemens,F. Gomes Naveca,Valdinete Alves do Nascimento,Fabrícia F. Nascimento,C Fernandes da Costa,Paola Cristina Resende,Alex Pauvolid-Corrêa,Marilda M. Siqueira,Christina Dold,Nigel J. Temperton,Tao Dong,Andrew J. Pollard,James C. Knight,D W Crook,Teresa Lambe,Elizabeth A. Clutterbuck,S Bibi,Amy Flaxman,M Bittaye,Sandra Belij-Rammerstorfer,Sarah C. Gilbert,T Malik,Miles W. Carroll,Paul Klenerman,Eleanor Barnes,Susanna Dunachie,Vicky L. Baillie,Vicky L. Baillie,N Serafin,N Serafin,Z Ditse,Z Ditse,K Da Silva,K Da Silva,Neil G. Paterson,Mark A. Williams,David Hall,Shabir A. Madhi,Shabir A. Madhi,M C Nunes,M C Nunes,P Goulder,Elizabeth E. Fry,Juthathip Mongkolsapaya,Juthathip Mongkolsapaya,Jingshan Ren,David I. Stuart,Gavin R. Screaton +65 more
TL;DR: In this paper, the ability of monoclonal antibodies and convalescent and vaccine sera to neutralize B.617.1 and B.1.2 has been studied.
Journal ArticleDOI
Functional SARS-CoV-2-Specific Immune Memory Persists after Mild COVID-19.
Lauren B. Rodda,Jason Netland,Laila Shehata,Kurt B Pruner,Peter A. Morawski,Christopher D. Thouvenel,Kennidy K Takehara,Julie Eggenberger,Emily A. Hemann,Hayley R Waterman,Mitchell L Fahning,Yu Chen,Yu Chen,Malika Hale,Malika Hale,Jennifer A Rathe,Caleb Stokes,Samuel Wrenn,Brooke Fiala,Lauren Carter,Jessica A. Hamerman,Neil P. King,Michael Gale,Daniel J. Campbell,Daniel J. Campbell,David J. Rawlings,Marion Pepper +26 more
TL;DR: Mild COVID-19 elicits memory lymphocytes that persist and display functional hallmarks of antiviral immunity, and SARS-CoV-2-specific memory lymphocyte exhibited characteristics associated with potent antiviral function.
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