SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor
Markus Hoffmann,Hannah Kleine-Weber,Simon Schroeder,Nadine Krüger,Tanja Herrler,Sandra Erichsen,Tobias S. Schiergens,Georg Herrler,Nai Huei Wu,Andreas Nitsche,Marcel A. Müller,Christian Drosten,Christian Drosten,Stefan Pöhlmann +13 more
TLDR
It is demonstrated that SARS-CoV-2 uses the SARS -CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming, and it is shown that the sera from convalescent SARS patients cross-neutralized Sars-2-S-driven entry.About:
This article is published in Cell.The article was published on 2020-04-16 and is currently open access. It has received 15362 citations till now. The article focuses on the topics: Proteases.read more
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Prevalence and predictors of death and severe disease in patients hospitalized due to COVID-19: A comprehensive systematic review and meta-analysis of 77 studies and 38,000 patients.
TL;DR: The prevalence of hypertension, diabetes, smoking history, and heart disease among the COVID-19 hospitalized patients in the US were substantially higher than that of the general US population, suggesting increased susceptibility to infection or disease progression for the individuals with comorbidities.
Journal ArticleDOI
Serological Assays Estimate Highly Variable SARS-CoV-2 Neutralizing Antibody Activity in Recovered COVID-19 Patients.
Larry L. Luchsinger,Brett P. Ransegnola,Daniel K. Jin,Frauke Muecksch,Yiska Weisblum,Weili Bao,Parakkal Jovvian George,Marilis Rodriguez,Nancy Tricoche,Fabian Schmidt,Chengjie Gao,Shabnam Jawahar,Mouli Pal,Emily Schnall,Huan Zhang,Donna Strauss,Karina Yazdanbakhsh,Christopher D. Hillyer,Paul D. Bieniasz,Paul D. Bieniasz,Theodora Hatziioannou +20 more
TL;DR: Levels of antibodies in convalescent plasma samples are measured using commercially available SARS-CoV-2 detection tests and in-house enzyme-linked immunosorbent assays and correlated serological measurements with NAb activity measured using pseudotyped virus particles, which offer the most informative assessment of antiviral activity of patient sera against viral infection.
Journal ArticleDOI
Hydroxychloroquine-mediated inhibition of SARS-CoV-2 entry is attenuated by TMPRSS2.
TL;DR: In this paper, the authors showed that the SARS-CoV-2 entry process is more dependent than that of SARS CoV-1 on TMPRSS2 expression.
Journal ArticleDOI
An infectivity-enhancing site on the SARS-CoV-2 spike protein targeted by antibodies
Yafei Liu,Wai Tuck Soh,Jun-ichi Kishikawa,Mika Hirose,Emi E. Nakayama,Songling Li,Miwa Sasai,Tatsuya Suzuki,Asa Tada,Akemi Arakawa,Sumiko Matsuoka,Kanako Akamatsu,Makoto Matsuda,Chikako Ono,Shiho Torii,Kazuki Kishida,Hui Jin,Wataru Nakai,Noriko Arase,Atsushi Nakagawa,Maki Matsumoto,Yukoh Nakazaki,Yasuhiro Shindo,Masako Kohyama,Keisuke Tomii,Koichiro Ohmura,Shiro Ohshima,Toru Okamoto,Masahiro Yamamoto,Hironori Nakagami,Yoshiharu Matsuura,Takayuki Kato,Masato Okada,Daron M. Standley,Tatsuo Shioda,Hisashi Arase +35 more
TL;DR: In this paper, a series of anti-spike monoclonal antibodies from coronavirus disease 2019 (COVID-19) patients were screened and found that some of the antibodies against the N-terminal domain induced the open conformation of RBD and thus enhanced the binding capacity of the spike protein to ACE2 and infectivity of SARS-CoV-2.
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Repurpose Open Data to Discover Therapeutics for COVID-19 Using Deep Learning.
Xiangxiang Zeng,Xiang Song,Tengfei Ma,Xiaoqin Pan,Yadi Zhou,Yuan Hou,Zheng Zhang,Kenli Li,George Karypis,Feixiong Cheng,Feixiong Cheng,Feixiong Cheng +11 more
TL;DR: There have been more than 2.2 million confirmed cases and over 120,000 deaths from the human coronavirus disease 2019 (COVID-19) pandemic, caused by the novel severe acute respiratory syndrome coro...
References
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TL;DR: The findings are consistent with person-to-person transmission of this novel coronavirus in hospital and family settings, and the reports of infected travellers in other geographical regions.
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