SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor
Markus Hoffmann,Hannah Kleine-Weber,Simon Schroeder,Nadine Krüger,Tanja Herrler,Sandra Erichsen,Tobias S. Schiergens,Georg Herrler,Nai Huei Wu,Andreas Nitsche,Marcel A. Müller,Christian Drosten,Christian Drosten,Stefan Pöhlmann +13 more
TLDR
It is demonstrated that SARS-CoV-2 uses the SARS -CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming, and it is shown that the sera from convalescent SARS patients cross-neutralized Sars-2-S-driven entry.About:
This article is published in Cell.The article was published on 2020-04-16 and is currently open access. It has received 15362 citations till now. The article focuses on the topics: Proteases.read more
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Expressions and significances of the angiotensin-converting enzyme 2 gene, the receptor of SARS-CoV-2 for COVID-19.
Jiewen Fu,Baixu Zhou,Lianmei Zhang,Kyathegowdanadoddi Srinivasa Balaji,Chunli Wei,Xiaoyan Liu,Hanchun Chen,Jiangzhou Peng,Junjiang Fu +8 more
TL;DR: The ACE2 is a functional receptor for SARS-CoV-2 and has a potential anti-tumor role in cancer, which may provide potential clues for further medical pathogenesis of COVID-19 and male fertility, and indicate the clinical significance of the role of the ACE2 gene in cancer.
Journal ArticleDOI
The association of smoking status with SARS-CoV-2 infection, hospitalisation and mortality from COVID-19: A living rapid evidence review with Bayesian meta-analyses (version 11)
TL;DR: Compared with never smokers, current smokers appear to be at reduced risk of SARS-CoV-2 infection while former smokers appear be at increased risk of hospitalisation, greater disease severity and mortality from COVID-19, however, it is uncertain whether these associations are causal.
Journal ArticleDOI
Genome-wide CRISPR screening identifies TMEM106B as a proviral host factor for SARS-CoV-2.
Jim Baggen,Leentje Persoons,Els Vanstreels,Sander Jansen,Dominique Van Looveren,Bram Boeckx,Vincent Geudens,Julie De Man,Dirk Jochmans,Joost Wauters,Els Wauters,Bart M. Vanaudenaerde,Diether Lambrechts,Johan Neyts,Kai Dallmeier,Hendrik Jan Thibaut,Maarten Jacquemyn,Piet Maes,Dirk Daelemans +18 more
TL;DR: It is discovered that SARS-CoV-2 requires the lysosomal protein TMEM106B to infect human cell lines and primary lung cells, and single-cell RNA-sequencing of airway cells from patients with COVID-19 demonstrated that TMEM 106B expression correlates with Sars-Cov-2 infection.
Journal ArticleDOI
Coronaviruses: Innate Immunity, Inflammasome Activation, Inflammatory Cell Death, and Cytokines.
TL;DR: This review focuses on the present understanding of innate immune responses, inflammasome activation, inflammatory cell death pathways, and cytokine secretion during SARS-CoV, MERS- CoV, and SARS -CoV-2 infection.
Posted ContentDOI
Camostat mesylate inhibits SARS-CoV-2 activation by TMPRSS2-related proteases and its metabolite GBPA exerts antiviral activity
Markus Hoffmann,Heike Hofmann-Winkler,Joan C. Smith,Joan C. Smith,Nadine Krüger,Lambert K. Sørensen,Ole Schmeltz Søgaard,Ole Schmeltz Søgaard,Jørgen B. Hasselstrøm,Michael Winkler,Tim Hempel,Lluís Raich,Simon Olsson,Takashi Yamazoe,Katsura Yamatsuta,Hirotaka Mizuno,Stephan Ludwig,Frank Noé,Frank Noé,Jason M. Sheltzer,Mads Kjolby,Mads Kjolby,Stefan Pöhlmann +22 more
TL;DR: It is shown that SARS-CoV-2 can employ TMPRSS2-related host cell proteases for activation and that several of them are expressed in viral target cells, however, entry mediated by these proteases was blocked by camostat mesylate.
References
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TL;DR: The findings are consistent with person-to-person transmission of this novel coronavirus in hospital and family settings, and the reports of infected travellers in other geographical regions.
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